Goat antirabbit igg peroxidase

Supernatants from FLC-4 cells incubated with gefitinib or from THP-1 cells incubated with the supernatants from FLC-4 cells incubated with gefitinib (20 μL) were loaded onto a 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel. The resolved proteins were then electro-transferred onto a polyvinylidene difluoride membrane (0.45 µm, Merck KGaA). High mobility group box (HMGB) 1 rabbit polyclonal antibody (10829-1-AP, Proteintech Group, Inc., Rosemont, IL, USA), HSP32 rabbit polyclonal antibody (10701-1-AP, Proteintech Group, Inc.), HSP40 rabbit polyclonal antibody (13174-1-AP, Proteintech Group, Inc.), HSP60 rabbit polyclonal antibody (15282-1-AP, Proteintech Group, Inc.), HSP70 rabbit polyclonal antibody (10995-1-AP, Proteintech Group, Inc.), HSP90 rabbit polyclonal antibody (13171-1-AP, Proteintech Group, Inc.), S100 calcium-binding protein (S100) A8 rabbit polyclonal antibody (GTX54721, GeneTex, Inc., Irvine, CA, USA) and S100A9 rabbit polyclonal antibody (GTX129575, GeneTex, Inc.) were used as the primary antibody and were detected by goat antirabbit IgG-peroxidase (Santa Cruz Biotechnology, Dallas, TX, USA). Bound peroxidase was visualized by using Luminata™ Classico Western HRP Substrates (Merck KGaA).