Pmig pp2aa

Manufactured by Addgene

The PMIG-PP2Aa is a laboratory equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Megfp c1, by Addgene (2 mentions) Pmig pp2ab, by Addgene (2 mentions) Pcdna3.1 drp1, by Addgene (2 mentions)

2 protocols using pmig pp2aa

Plasmid Cloning and Lentiviral Production

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pMIG-PP2Aa (#10884), pMIG-PP2Ab (#13804) and pcDNA3.1-Drp1 (#34706) plasmids were purchased from Addgene and subcloned into mEGFP-C1 (#54759) and pCMV-Myc-3B vectors. RalA^WT, RalA^G23V and RalA^S28N plasmids were subcloned into a pLVX vector with 3× Flag tag for lentiviral production.

Xia W., Veeragandham P., Cao Y., Xu Y., Rhyne T.E., Qian J., Hung C.W., Zhao P., Jones Y., Gao H., Liddle C., Yu R.T., Downes M., Evans R.M., Rydén M., Wabitsch M., Wang Z., Hakozaki H., Schöneberg J., Reilly S.M., Huang J, & Saltiel A.R. (2024). Obesity causes mitochondrial fragmentation and dysfunction in white adipocytes due to RalA activation. Nature Metabolism, 6(2), 273-289.

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Molecular Cloning of RalA Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols

pMIG-PP2Aa (#10884), pMIG-PP2Ab (#13804), and pcDNA3.1-Drp1 (#34706) plasmids were purchased from Addgene and subcloned into mEGFP-C1 (#54759) and pCMV-Myc-3B vectors. RalA^WT, RalA^G23V, and RalA^S28N plasmids were subcloned into a pLVX vector with 3x Flag tag for lentiviral production.

xia W., Veeragandham P., Cao Y., Xu Y., Rhyne T., Qian J., Hung C.W., Zhao P., Jones Y., Gao H., Liddle C., Yu R., Downes M., Evans R., Ryden M., Wabitsch M., Reilly S., Huang J, & Saltiel A. (2023). Obesity-dependent increase in RalA activity disrupts mitochondrial dynamics in white adipocytes. Research Square.

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