The tumor tissue removed from each mouse was fixed in 4% paraformaldehyde and embedded in paraffin for 5 μm-thick sections. After being deparaffinized and rehydrated, the sections were treated with sodium citrate buffer (pH = 6), and the microwave was used for antigen retrieval. The activity of endogenous peroxidase was blocked with 3% H2O2 in methanol. The sections were then incubated with anti-CD3 monoclonal antibody (Abcam, Cambridge, UK), anti-CD4 monoclonal antibody (Abcam, Cambridge, UK), and anti-CD8 monoclonal antibody (Abcam, Cambridge, UK) at 4 °C overnight, respectively. Afterwards, the sections were stained with HPR-conjugated secondary antibody, and the positive reactions were visualized with diaminobenzidine (DAB). Finally, the sections were counterstained with Mayer’s hematoxylin. Digital images of the stained sections were obtained in five randomly selected fields both at the interior regions and the margin of tumors using a fluorescence microscope. The positive cell numbers were counted and the results from the five areas were averaged and used in the statistical analysis.
Anti cd4 monoclonal antibody
Manufactured by Abcam
Sourced in United Kingdom
Anti-CD4 monoclonal antibody is a laboratory reagent used to detect and analyze CD4-expressing cells. It functions by specifically binding to the CD4 cell surface receptor, which is expressed on a subset of T cells and other immune cells. This antibody can be used in various immunological techniques, including flow cytometry, immunohistochemistry, and Western blotting, to identify and study CD4-positive cell populations.
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Lab products found in correlation
2 protocols using anti cd4 monoclonal antibody
1
Immunohistochemical Analysis of Tumor-Infiltrating T Cells
2
Immunohistochemical Analysis of Tumor-Infiltrating Immune Cells
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The tumor tissue removed from each mouse was xed in 4% paraformaldehyde and embedded in para n for 5 μm-thick sections. After being depara nized and rehydrated, the sections were treated with sodium citrate buffer (pH = 6), and the microwave was used for antigen retrieval. The activity of endogenous peroxidase was blocked with 3% H 2 O 2 in methanol. The sections were then incubated with anti-CD3 monoclonal antibody (Abcam, Cambridge, UK), anti-CD4 monoclonal antibody (Abcam, Cambridge, UK), and anti-CD8 monoclonal antibody (Abcam, Cambridge, UK) at 4°C overnight, respectively. Afterwards, the sections were stained with HPR-conjugated secondary antibody, and the positive reactions were visualized with diaminobenzidine (DAB). Finally, the sections were counterstained with Mayer's hematoxylin. Digital images of the stained sections were obtained in ve randomly selected elds both at the interior regions and the margin of tumors using a uorescence microscope. The positive cell numbers were counted and the results from the ve areas were averaged and used in the statistical analysis.
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