All experimental procedures were performed in accordance with institutional guidelines and approved by the Ani-mal Experimentation Ethics Committee of the Chinese Uni-versity of Hong Kong. c-kit+ CPCs were isolated from 2-month-old male wild-type FVB mice. All c-kit+ adult CPCs were isolated and cultured as previously described (18 (link)). To activate differentiation, CPCs were incubated in α-mini-mal essential medium containing 10 nM dexamethasone for up to 7 days (19 (link)). To inhibit Drp1-mediated mitochondrial fragmentation, either 10 μM or 50 μM mdivi-1 (Sigma) was administered once at Day 0 and again at Day 2 of differentiation (20 (link)). To inhibit calcineurin, either 1 μM or 5 μM ciclosporin-A (CsA) (Sigma) was administered once at Day 0 and again at Day 2 of differentiation (17 (link)).
Ciclosporin a csa
Manufactured by Merck Group
Sourced in United States
Ciclosporin A (CsA) is an immunosuppressant drug used as a pharmaceutical active ingredient. It is a cyclic polypeptide that acts by inhibiting the activation of T-cells. CsA is utilized in various applications, including organ transplantation, autoimmune disorders, and inflammatory conditions.
Automatically generated - may contain errors
Lab products found in correlation
Mdivi 1, by Merck Group (1 mentions)
Horseradish peroxidase hrp conjugated igg, by Jackson ImmunoResearch (1 mentions)
N nitro l arginine methyl ester l name, by Merck Group (1 mentions)
Cytochrome c cyt c, by Abcam (1 mentions)
Horseradish peroxidase conjugated igg, by Jackson ImmunoResearch (1 mentions)
β actin, by Abcam (1 mentions)
3 protocols using ciclosporin a csa
1
Cardiac Progenitor Cell Differentiation
2
Evaluating Endothelial Cell Function
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Adenovirus pAD/DDAHII and pAD/DDAHII-shRNA were from GeneChem Co., Ltd. (Shanghai, China). Iron dextran (iron-D), dextran (Dex), phenylephrine (PE), sodium nitroprusside (SNP), acetylcholine (Ach), L-arginine (L-Arg), Edaravone (Eda), N-nitro-l-arginine methyl ester (l-NAME), and ciclosporin A (CsA) were purchased from Sigma-Aldrich (cat. nos. D8517, D9885, P1240000, PHR1423, PHR1546, A5006, M70800, N5751, and C1832, respectively, St. Louis, MO, USA). Antibodies directed against DDAHII, eNOS, eNOS phospho-S1177, cytochrome c (cyt c), COX4, and β-actin were purchased from Abcam (cat. no. ab1383, ab5589, ab184154, ab16381, ab13575, and ab8229, respectively, Cambridge, UK). Horseradish peroxidase- (HRP-) conjugated IgG was from Jackson ImmunoResearch (cat. no. 107-035-142, West Grove, PA, USA).
Human umbilical vein endothelial cells (HUVECs) were purchased from the China Infrastructure of Cell Line Resources (Shanghai, China). Male C57BL/6J mice, 8-10 weeks old, weighing 20-22 g, were provided by the Animal Center of Nanchang University (Nanchang, China).
All experiments were performed following the National Institutes of Health (NIH) Guidelines for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996) and were approved by the Ethics Committee of Nanchang University (Nanchang, China) (No. 2017-0306 (in vitro) and 2017-0305 (in vivo)).
Human umbilical vein endothelial cells (HUVECs) were purchased from the China Infrastructure of Cell Line Resources (Shanghai, China). Male C57BL/6J mice, 8-10 weeks old, weighing 20-22 g, were provided by the Animal Center of Nanchang University (Nanchang, China).
All experiments were performed following the National Institutes of Health (NIH) Guidelines for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996) and were approved by the Ethics Committee of Nanchang University (Nanchang, China) (No. 2017-0306 (in vitro) and 2017-0305 (in vivo)).
3
Adenoviral eNOS Modulation in Endothelial Cells
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Adenovirus pAD/eNOS was from GeneChem Co., Ltd (Shanghai, China). Dox, phenylephrine (PE), sodium nitroprusside (SNP), acetylcholine (Ach), Eda, N-nitro-l-arginine methylester (l-NAME), and ciclosporin A (CsA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies directed against eNOS, eNOS phospho-S1177, cytochrome C (cyt c), and β-actin were purchased from Abcam (Cambridge, UK). Horseradish peroxidase-conjugated IgG was from Jackson Immuno Research (West Grove, PA, USA).
Human umbilical vein endothelial cells (HUVECs) were purchased from the China infrastructure of cell line resources (Shanghai, China). Male C57BL/6J mice, 8-10 weeks old, weighing 20-22 g, were provided by the Animal Center of Nanchang University (Nanchang, China). All experimental protocols were performed following the National Institutes of Health (NIH) Guidelines for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996) and approved by the Ethics Committee of Nanchang University (Nanchang, China, No. 2018-0116).
Human umbilical vein endothelial cells (HUVECs) were purchased from the China infrastructure of cell line resources (Shanghai, China). Male C57BL/6J mice, 8-10 weeks old, weighing 20-22 g, were provided by the Animal Center of Nanchang University (Nanchang, China). All experimental protocols were performed following the National Institutes of Health (NIH) Guidelines for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1996) and approved by the Ethics Committee of Nanchang University (Nanchang, China, No. 2018-0116).
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