Eclusys trab

Free triiodothyronine (FT 3 ) and free thyroxine (FT 4 ) levels were measured by an electrochemiluminescence immunoassay (ECLIA) (ECLusys FT 4 , Roche Diagnostics GmbH, Mannheim, Germany; manufacturer's reference limits: 2.2-4.3 pg/mL, 0.8-1.6 ng/ dL respectively). The TSH level was measured by an ECLIA (ECLusys TSH; Roche Diagnostics GmbH, Mannheim, Germany; manufacturer's reference limits: 0.2-4.5 mIU/L). Based on the results of our previous study of a large population, the reference intervals for FT 3, and FT 4, and TSH at 4-12 weeks of gestation were 2.01-4.9 pg/mL, 0.77-1.91 ng/dL, and 0.01-3.35 mIU/L, respectively.
TPOAb and TgAb were determined by an ECLIA performed with Roche ECLusys Anti-Tg and Anti-TPO (Roche Diagnostics GmbH, Mannheim, Germany; reference values were: TPOAb, ≤28 IU/mL; TgAb, ≤40 IU/mL). Until September 30, 2008, TRAb levels were determined with a solid-phase immunoradioreceptor assay kit (RSR, Cardiff, UK; TRAb-CT; reference values <10%), but since October 1, 2008, they have been determined with an electrochemiluminescence immunoassay kit (ECLusys TRAb; Roche Diagnostics GmbH, Mannheim, Germany; reference values <2.0

Blood samples were collected from all patients. FT4, FT3, and TSH values were measured using a commercial electrochemiluminescence immunoassay (Roche Diagnostics, Tokyo; FT4: normal range, 1.00-1.70 ng/ dL; FT3: normal range, 2.40-4.50 pg/mL; TSH: normal range, 0.56-4.30 μIU/mL). Serum TRAb was measured using a two-step radioreceptor assay DYNO test TRAb (Yamasa Corp, Tokyo; normal range, <1.0 IU/L; detectable level, ≥ 1.0 IU/L) between January 1, 2009 and May 12, 2011 for 61 patients, and another two-step radioreceptor assay ECLusys TRAb (Roche Diagnostics, Tokyo; normal range, <2.0 IU/L; detectable level, ≥ 0.3 IU/L) between May 14, 2011 and December 31, 2013 for 33 patients. TRAb levels measured using ECLusys TRAb were converted to DYNO test TRAb equivalents using the following formula: ECLusys TRAb (IU/L) = 1.08 × [DYNO test TRAb (IU/L)] -0.55 (Supplemental Fig. 1). This formula was based on Schott's formula [3] . Most patients with painless thyroiditis had undetectable levels of TRAb. These TRAb values were set at 1.0 IU/L for analysis. Thyroid autoantibodies [thyroglobulin antibody (TgAb) and thyroid peroxidase antibody (TPOAb)] were measured using a radioimmunoassay (Cosmic Corp, Tokyo).