Dipalmitoylphosphatidylcholine dppc

Biotin, streptavidin, bovine serum albumin, Tween-20, tris base, sodium chloride, cholesterol, chloroform, sulforhodamine B (SRB), noctyl-beta-d-glucopyranoside (n-OG) and sucrose were purchased from Sigma-Aldrich. Dipalmitoylphosphatidylcholine (DPPC), dipalmotoylphosphatidylglycerol (DPPG) and the liposome extruder were purchased from Avanti Polar Lipids. Sepharose CL4B was purchased from GE healthcare. Maxisorp 96-well black plate, 200 nm filter membranes were purchased from Whatman. Aptamers are synthesized by IDT (Integrated DNA Technologies, USA). Two aptamers (ST-21: 5′-ATTGACCGCTGT GTGACGCAACACTCAAT-3′ and ST-21M: 5′-ATTGACCTCTGTGTGACGCAA CACTCAAT-3′) were used in this study.

The 5-FU and cholesterol (CHO) were purchased from Beijing Mesochem Technology CO. Ltd. (Beijing, China). 5-FUC was prepared in our laboratory, Kayyali Chair for Pharmaceutical Industries (King Saud University, Riyadh, Saudi Arabia). Dipalmitoylphosphatidylcholine (DPPC) was purchased from Avanti Polar Lipids, Inc. (Toronto, Canada). Chromatography grade methanol (MeOH) and chloroform (CHCl3) were obtained from Sigma Aldrich (St. Louis, MO, USA). All other chemicals and reagents were of analytical grade and used without any further purification.

Dipalmitoylphosphatidylcholine (DPPC) and the spin‐labelled lipids 1‐palmitoyl‐2‐(16‐ doxyl stearoyl) phosphatidylcholine (16‐PC) and 1‐palmitoyl‐2‐(5‐doxyl stearoyl) phosphatidylcholine (5‐PC) were purchased from Avanti Polar Lipids (Alabaster, AL). Cholesterol and the 3β‐doxyl‐5α‐ cholestane (CSL) were from Sigma (UK). Measured stock solutions of the lipids and spin‐labels in chloroform/methanol (2 : 1 v/v) were mixed in a glass bottle, evaporated to dryness under a stream of N₂ and further dried in a vacuum desiccator for 3 hrs.7b The concentration of spin‐label was circa 2.0 mol % of the lipids. Phosphate buffered saline, warmed to 60 °C, was added to the lipid film and the mixture vortexed vigorously to give a 40 mM lipid suspension. Samples were incubated in an anaerobic glovebox for 1 hour and transferred to 1.1 mm (inner diameter) capillaries under anaerobic conditions.11b Each capillary was sealed with capillary wax (Hampton Research).

Dipalmitoyl phosphatidyl choline (DPPC), poly(ethylene glycol) (PEG-2000) covalently linked to distearoyl phosphatidyl ethanolamine (DSPE-PEG), rhodamine covalently linked to phosphatidyl ethanolamine (Rho-PE) and the functionalized DSPE-PEG phospholipids with folate (DSPE-PEG-FA) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Cholesterol (Chol), and phosphate buffered saline (PBS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Pan was purchased from Selleckchem (Houston, TX USA, Cat # S1030). All other reagents were of analytical grade.

Gelatin, L-phenylalanine (99%), L-tyrosine (99%), and naringin (98%) were purchased from Adamas-beta(Shanghai,China).Palmitic acid (>97%), stearic acid (>98%), linoleic acid (>97%), and D-(+)-raffinose(>98%) were purchased from TCI Chemical Industries (Shanghai, China).Abscisic acid (ABA, 98%) were purchased from Sigma-Aldrich(St. Louis, USA).Dipalmitoyl phosphatidylcholine (DPPC) was purchased from Avanti Polar Lipids (Alabama, USA). Indolebutyric acid (99%)and α-cyclodextrin (≥98%) were purchased from Yuanye Biotechnology (Shanghai, China).Glycerol trioleate (99%) and 2,5-dihydroxybenzoic acid (DHB, 99%)were purchased from J&K Scientific (Beijing, China).Paeoniflorin (98%), sucrose (AR), and L-glutamic acid (≥99%) were purchased from Titan Technology (Shanghai, China).Ethanol (absolute, AR grade) was purchased from Sinopharm (Beijing, China).Methanol (HPLC grade) and dichloromethane (AR grade) were purchased from Boer Chemical Reagent (Shanghai, China), deionized water was obtained from Milli-Q Water Purification System (Millipore, Billerica, MA, USA).
Tissue-Tek OCT compound was purchased from Sakura Finetek (CA, USA). Microscope cover glasses (22mm × 22mm) were purchased from Citotest Scientific (Nanjing, China).We purchased fresh sweet corn from a local supermarket. Wheat seeds (Jimai 22) were from Crop Research Institute, Shandong Academy of Agricultural Sciences.

Peptide synthesis reagents were purchased from AnaSpec (Fremont, CA, USA), high performance liquid chromatography (HPLC) solvents from Fisher Scientific (Pittsburgh, PA, USA), and all other chemicals from MilliporeSigma (St. Louis, MO, USA). Dipalmitoyl phosphatidylcholine (DPPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and palmitoyl-oleoyl-phosphatidylglycerol (POPG) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The clinical porcine surfactant Poractant Alfa (Curosurf^®) was obtained from Chiesi Farmaceutici, Parma, Italy.