Smi 31r

Manufactured by Fortrea

The SMI-31R is a compact and versatile lab equipment designed for various scientific applications. It features a durable construction and precise measurement capabilities, making it a reliable tool for researchers and laboratory professionals. The core function of the SMI-31R is to provide accurate and reliable data collection, without any specific interpretation or extrapolation on its intended use.

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Lab products found in correlation

Ab107216, by Abcam (1 mentions) Sc 188, by Santa Cruz Biotechnology (1 mentions) Mca839g, by Bio-Rad (1 mentions) Smi 32r, by Fortrea (1 mentions) Dm6000b microscope, by Leica (1 mentions) Olig2, by Merck Group (1 mentions) Smi 94r, by Fortrea (1 mentions) A0136, by Agilent Technologies (1 mentions) A0062, by Agilent Technologies (1 mentions) Envision flex kit, by Agilent Technologies (1 mentions) A0063, by Agilent Technologies (1 mentions) Glass coverplates, by Thermo Fisher Scientific (1 mentions) Autostainer, by Agilent Technologies (1 mentions)

4 protocols using smi 31r

Immunohistochemical Staining Protocol

Cited 1 time

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Primary antibodies used in this study include: Anti-βIII tubulin (abcam, ab107216, 1:1000 dilution)(Rinkevich et al. 2014 (link))), anti-ATF3 (C-19), (Santa Cruz SC-188, 1:1000 dilution, (Carozzi et al. 2013 (link))), anti-α-tubulin (Millipore, 04-1117, 1:100 dilution (Zhang et al. 2011 (link))), anti-acetylated tubulin(Cell Signal, 5335, 1:800 dilution, (Creppe et al. 2009 (link))), anti-EB1 (Millipore, AB6057, 1:500 dilution, (Vitre et al. 2008 (link))), anti-EB3 (Santa Cruz, SC-101475, 1:200 dilution, (Levy et al. 1994 (link))) and anti-phosphoneurofilament (Covance, SMI-31R, 1:2000 dilution, (Choi et al. 2008 (link))). Secondary antibodies used in this study included: Cy2-conjugated donkey anti-rabbit (711-225-152), Cy2-conjugated donkey anti rat (712-225-153), Cy3-conjugated donkey anti-chicken (703-165-155), and Cy5-conjugated donkey anti-mouse (715-175-151) (all purchased from Jackson Immunoresearch, (West Grove, PA)).

Benbow S.J., Wozniak K.M., Kulesh B., Savage A., Slusher B.S., Littlefield B.A., Jordan M.A., Wilson L, & Feinstein S.C. (2017). Microtubule Targeting Agents Eribulin and Paclitaxel Differentially Affect Neuronal Cell Bodies in Chemotherapy Induced Peripheral Neuropathy. Neurotoxicity research, 32(1), 151-162.

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Qualitative Analysis of VWM Brain Tissue

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From our collection of VWM patient brain tissue, tissue of 14 patients, involving seven different brain regions (frontal, temporal, parietal, and occipital lobes; corpus callosum; internal capsule; and cerebellum) was analyzed by two different investigators. However, being archive material, only qualitative microscopic analysis of previously stained sections was possible and no additional stains for quantification could be performed. Formalin‐fixed, paraffin−embedded tissue sections (6 μm thick) were deparaffinized and stained with haematoxylin and eosin or Bodian. For immunohistochemistry, heat−induced antigen retrieval was performed in 0.01 mol/L citrate buffer (pH 6) in microwave at low setting, followed by incubation with antibodies against NF (MON3004; 1:50; Monosan), MBP (MAB387; 1:50; EMD Millipore), proteolipid protein (MCA839G; 1:3,000; AbD Serotec), phosphorylated neurofilament (SMI‐31R, 1:1000, Covance), or non‐phosphorylated neurofilament (SMI‐32R, 1:1000, Covance) and subsequently a horseradish peroxidase secondary antibody. Immunopositivity was detected with 3,3′‐Diaminobenzidine (DAB) as chromogen. Images were acquired on a Leica DM6000B microscope (Leica Microsystems).

Klok M.D., Bugiani M., de Vries S.I., Gerritsen W., Breur M., van der Sluis S., Heine V.M., Kole M.H., Baron W, & van der Knaap M.S. (2018). Axonal abnormalities in vanishing white matter. Annals of Clinical and Translational Neurology, 5(4), 429-444.

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Myelin and Neuronal Marker Visualization in Mouse Tissues

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Mice were anesthetized and perfused intracardially with PBS and then 4% paraformaldehyde. Brains were dissected out, postfixed with 4% paraformaldehyde at 4°C for 1h and processed for vibratome section (50 μm). Pregnant mice at indicated gestational days were anesthetized and perfused using PBS. Embryos were dissected out and fixed with 4% paraformaldehyde at 4°C overnight and processed for frozen sections (16 μm).
To visualize PNS myelin, freshly extracted mouse sciatic nerves segments (10 mm) were teased on poly-L-lysine coated microscope slides, using fine needles to gently separate the nerve fibers. The preparations were then dried overnight at room temperature, washed in PBS 3×5 min and finally mounted in 50% glycerol/PBS. Immunostaining with antibodies against Olig2 (Millipore), PDFGRα (BD Bioscience, 558774), Tau (Sigma T6402), CC1 (Oncogene Research, OP80) and MBP (Covance, SMI-94R) or Neurofilament (Covance, SMI-31R), which labels a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in axons, was performed on tissue or cell cultures using standard protocols along with corresponding fluorescent secondary antibodies (Jackson lab). Topro3 (Molecular Probe) was included to label nuclei. In situ hybridization was carried out as previously described (Yu et al., 2013 (link)).

Deng Y., Kim B., He X., Kim S., Lu C., Wang H., Cho S.G., Hou Y., Li J., Zhao X, & Lu Q.R. (2014). Direct Visualization of Membrane Architecture of Myelinating Cells in Transgenic Mice Expressing Membrane-Anchored EGFP. Genesis (New York, N.Y. : 2000), 52(4), 341-349.

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Immunohistochemical Analysis of Neuronal Markers

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Postsurgically, the paraffin sections were routinely processed for histopathology. The following antibodies and dilutions were applied on 3‐µm tissue sections mounted on negatively charged glass slides: C1q (Dako, A0136, 1:200), C3c (Dako, A0062, 1:100), C3d (Dako, A0063, 1:400), synaptophysin (Syn; Dako, DAK‐SYNAP, 1:500), SMI31 (Covance, SMI31R, 1:25 000), SMI32 (Sternberger, 1:1000), vimentin (Dako, V9, 1:50), and Map2 (Millipore, AP20, 1:8000). Tissue was pretreated with either proteinase K (C1q, C3c, C3d) or pH6 (Syn, Map2, SMI32, SMI31). As a detection system, the EnVision FLEX+ kit (Dako) was utilized and diaminobenzidine as chromogen, executed either with an autostainer (Dako) or via cover plates (Thermo Fisher Scientific glass cover plates). The paraffin sections were counterstained with hematoxylin.

Gruber V., Luinenburg M.J., Colleselli K., Endmayr V., Anink J.J., Zimmer T.S., Jansen F., Gosselaar P., Coras R., Scholl T., Blumcke I., Pimentel J., Hainfellner J.A., Höftberger R., Rössler K., Feucht M., van Scheppingen J., Aronica E, & Mühlebner A. (2021). Increased expression of complement components in tuberous sclerosis complex and focal cortical dysplasia type 2B brain lesions. Epilepsia, 63(2), 364-374.

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