and catalase (lyophilized powder from bovine liver 2000–5000
units/mg protein) were purchased from Sigma-Aldrich. Na2SO3 anhydride (85–90%), FeSO4·7H2O, H2O2 (33% w/v), ascorbic acid, 1,10-phenanthroline
1-hydrate, H2SO4 (96% w/w), and UHPLC-grade
methanol and acetonitrile were obtained from AppliChem-Panreac. Formic
acid (80% w/w) was purchased from VWR Chemicals. Ultrapure water was
prepared with a Merck Milli-Q system. The zone of inhibition tests
was made using E. coli CECT 101 strain,
employing tryptone soya and Mueller–Hinton broth, both provided
by Scharlau.