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2 protocols using dextrose

1

Pseudomonas aeruginosa P4 Cultivation Protocol

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Bacterial strain, culture conditions and reagents Isolate P. aeruginosa P4 [MCC No. 2365 ] used as the model strain in all the experiments, was kindly gifted by Prof. G. Naresh Kumar, Department of Biochemistry, The M S University of Baroda, India. The strain was grown and maintained on Pseudomonas agar (Hi-Media Laboratories, Mumbai, India) at 30°C, as required. The culture media, dextrose, Tris base and other routine analytical-grade salts and reagents were procured from SD Fine Chemicals Ltd., Hi-media Laboratories and Merck Life Science Pvt. Ltd. Speci c molecular biology reagents like RNase, Taq DNA polymerase with its buffer and dNTPs, lysozyme, diethyl pyrocarbonate (DEPC), RNase Zap solution, Trizol etc. were procured from Sigma Aldrich.
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2

Synthesis of Nanocrystalline Materials

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Cadmium acetate dihydrate (Sigma-Aldrich,
98%), myristic acid (Sigma-Aldrich, 98%), selenium (Se, Sigma-Aldrich,
99.99%), tellurium (Te, SigmaAldrich, 99.99%), copper chloride dihydrate
(ACS reagent, 99.8%), zinc acetate trihydrate (Zn(CH3COO)2·2H2O, Sigma-Aldrich, 98%), sodium borohydride
(NaBH4, Sigma-Aldrich, 96%), trioctylphosphine (TOP, Sigma-Aldrich,
technical grade, 90%), 1-octadecene (ODE, Sigma-Aldrich, technical
grade, 90%), oleylamine (technical grade, 70%), sulfur (S, Sigma-Aldrich,
<99.5%), oleic acid (technical grade, Sigma-Aldrich, 90%), cadmium
oxide (Sigma-Aldrich, 99.9%), and lead oxide (Alfa Aesar, 99.9%).
Hexane (AR grade, 99%), 1,4-butanediol (90%, AR), dextrose (95% anhydrous,
AR), HCl (99%), and HNO3 (98%) were purchased from SD Fine
Chemicals. All chemicals were used without further purification.
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