Thymuses from untreated and SL-TBI treated mice were harvest, enzymatically digested and stained with cell surface markers for thymus populations. Cells were further stained for caspase 1 cleavage with Caspase-1 (active) Staining Kit (Abcam, ab219935), or fixed for caspase 3 analysis using Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate) (Cell signaling, 9669S). Apoptosis and pyroptosis was assessed by adding Annexin V-FITC (Biolegend, 640906), Annexin V binding buffer (BioLegend, 422201) and Propidium Iodide (Invitrogen, BMS500PI). Gasdermin D was measured in freshly isolated thymocytes using Gasdermin D (mouse) ELISA Kit (Adipogen Life Sciences, AG-45B-0011-KI01). Lactate dehydrogenase was assessed from the supernatant of harvested thymocytes using Lactate Dehydrogenase assay (Abcam, ab102526).
Gasdermin d mouse elisa kit
Manufactured by Adipogen
Sourced in Switzerland
The Gasdermin D (mouse) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of mouse Gasdermin D protein in cell culture supernatants, cell lysates, and other biological fluids.
Automatically generated - may contain errors
Lab products found in correlation
Caspase 1 active staining kit, by Abcam (1 mentions)
Annexin 5 fitc, by BioLegend (1 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
Annexin 5 binding buffer, by BioLegend (1 mentions)
Alexa fluor 488 conjugate, by Cell Signaling Technology (1 mentions)
Ab208348, by Abcam (1 mentions)
2 protocols using gasdermin d mouse elisa kit
1
Thymic Apoptosis and Pyroptosis Evaluation
2
Thapsigargin-Induced Hepatocyte Stress
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Primary mouse hepatocytes were treated with thapsigargin (final concentration: 2 or 5 μM) and the culture supernatants were collected and centrifuged to remove nonadherent cells. The concentrations of mouse TNF (Ab208348; Abcam, Cambridge, MA) and mouse IL-6 (M6000B; R&D Systems, Inc., Minneapolis, MN) were determined according to manufacturer’s instructions. The serum and liver gasdermin D levels were determined using Gasdermin D (mouse) ELISA Kit (AG-45B-0011-K101; AdipoGen Life Science, Switzerland) according to manufacturer’s instructions.
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