Genes encoding for fusion proteins were assembled as a multicomponent cloning cassette from annealed oligonucleotides (IDT DNA) containing these elements (in order): BglII or NheI or BamHI — Secretion Signal/FLAG tag — HindIII — (αDTX or DTX-K or CONK1) — KpnI — Linker — LOV2-Jα(404–546) — NotI — PDGF-R-mCherry — XbaI or EcoRI. PDGF-R-mcherry was derived from pFU-MVIIA-PC (Addgene)10 (link). See Table 1 for sequence details. This cassette was inserted into the mammalian expression vector pcDNA3.1(+) (Invitrogen) using NheI / Xba restriction sites or a lentiviral vector containing the CAMKII promoter35 (link),36 (link) using BamHI / EcoRI sites. The respective genes coding for rat Kv1.2 (Kv1.2), rat Kv1.1 (Kv1.1) or Shaker were amplified from Kv1.2-pBluescript, Shaker-pBluescript (gifts from Roderick Mackinnon), or BacMam Kv1.1 (Invitrogen) and inserted into pcDNA3.1(+) or pEGFP-N3 using BamHI / EcoRI or NheI/EcoRI, respectively. Both αDTX-lumitoxin and Kv1.2 cassettes were also inserted into the bidirectional expression vector pBI-CMV1 (Clontech) using BglII / XbaI and BamHI / NotI sites respectively, to drive expression from the same plasmid.
Bacmam kv1
Manufactured by Thermo Fisher Scientific
The BacMam Kv1.1 is a laboratory equipment product offered by Thermo Fisher Scientific. It is a voltage-gated potassium channel that can be expressed in mammalian cells using a baculovirus expression system. The core function of the BacMam Kv1.1 is to enable the study of potassium channel activity and related cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bacmam kv1
1
Engineered Optogenetic Fusion Proteins
2
Engineered Optogenetic Fusion Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genes encoding for fusion proteins were assembled as a multicomponent cloning cassette from annealed oligonucleotides (IDT DNA) containing these elements (in order): BglII or NheI or BamHI — Secretion Signal/FLAG tag — HindIII — (αDTX or DTX-K or CONK1) — KpnI — Linker — LOV2-Jα(404–546) — NotI — PDGF-R-mCherry — XbaI or EcoRI. PDGF-R-mcherry was derived from pFU-MVIIA-PC (Addgene)10 (link). See Table 1 for sequence details. This cassette was inserted into the mammalian expression vector pcDNA3.1(+) (Invitrogen) using NheI / Xba restriction sites or a lentiviral vector containing the CAMKII promoter35 (link),36 (link) using BamHI / EcoRI sites. The respective genes coding for rat Kv1.2 (Kv1.2), rat Kv1.1 (Kv1.1) or Shaker were amplified from Kv1.2-pBluescript, Shaker-pBluescript (gifts from Roderick Mackinnon), or BacMam Kv1.1 (Invitrogen) and inserted into pcDNA3.1(+) or pEGFP-N3 using BamHI / EcoRI or NheI/EcoRI, respectively. Both αDTX-lumitoxin and Kv1.2 cassettes were also inserted into the bidirectional expression vector pBI-CMV1 (Clontech) using BglII / XbaI and BamHI / NotI sites respectively, to drive expression from the same plasmid.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!