Ultratek hrp anti polyvalent lab pack

Epstein-Barr virus association was evaluated by EBERs in situ hybridization (EBER-ISH) and immunohistochemistry. EBER-ISH was performed on FFPE tissue sections using fluorescein isothiocyanate (FITC)-conjugated EBERs oligonucleotides as probes, following the manufacturer’s instructions (Dako). A monoclonal antibody anti-FITC labeled with alkaline phosphatase was used for the detection of EBERs probes (Dako), as described^{29 (link)}.
Immunostaining was used to localize viral LMP1 expression in tumor cells, using monoclonal antibodies CS1-4 (Dako). IHC detection of primary antibody was carried out using a universal streptavidin–biotin complex-peroxidase detection system (UltraTek HRP Anti- Polyvalent Lab Pack, ScyTek, Logan, Utah, USA) according to the manufacturer’s instructions^{29 (link)}.

BrC cells were stained with a rabbit monoclonal primary antibody anti-Estrogen Receptor (SP1, REF 790–4325), anti-Progesterone Receptor (1E2, REF 790–4296), and anti HER-2/neu (4B5, REF 790–2991) all from Ventana Medical Systems, Inc. (Ventana) and mouse monoclonal Androgen Receptor (F39.4.1, REF AM256-2ME) from Biogenex. Immunocytochemistry was performed using the UltraTek HRP Anti-Polyvalent Lab Pack from ScyTek Laboratories Inc. (REF UHP125), following the manufacturer's recommended procedure. Diaminobenzidine was used as the chromogen, and hematoxylin to counterstain. Images were acquired on a Zeiss Axioskop2Mot microscope through a Plan Neofluor 40× objective lens, using a color HVD-30 digital camera (Hitachi).

IHC for LMP1 (mAb CS1–4, Dako), LMP2A (clone 15F9, Abcam, Cambridge, UK), and EBNA2 (clones 1E6 and R3, kindly provided by Dr. Kremmer, Institut fur Immulogie, Munchen, Germany) were used to detect and localize EBV latent protein expression in tonsils and HL biopsies. Antigen unmasking with sodium citrate buffer (pH 6) in a microwave oven for 10 min was performed. All antibodies were incubated overnight at 4 °C. IHC detection primary antibody was carried out using a universal streptavidin–biotin complex-peroxidase detection system (UltraTek HRP Anti-Polyvalent Lab Pack, ScyTek, West Logan, UT, USA) according to the manufacturer’s instructions. In the tonsillectomies, LMP1 expression was employed in order to discriminate EBV+ and EBV-GC in all cases, given the fact that we have previously observed LMP1+ expression in all EBERs+ cases [10 (link)]. Once determined, LMP2A and EBNA2 expression and microenvironment compositions around infected and noninfected GC were compared.