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Collagen coated 96 well plates

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Collagen coated 96 well plates are laboratory equipment used for cell culture applications. The plates feature a surface coated with the extracellular matrix protein collagen, which promotes cell adhesion and growth. These plates provide a standardized platform for various cell-based assays and experiments.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Ethylene diamine tetraacetic acid (edta), by AppliChem (1 mentions)

2 protocols using collagen coated 96 well plates

1

Immunofluorescence Staining of CMT-64 Cells

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CMT-64 cells were seeded on collagen coated 96 well plates (Greiner) for 24 h then fixed with formaldehyde. CMT-64 cells were stained using Nessy-1 mAb (ALX-804–840, Enzo Life Sciences) or mouse IgG1 isotype control mAb (ADI-SAD-600, Enzo Life Sciences). DAPI (D1306, Life Technologies) was used to counterstain the cell nuclei. An Alexa Fluor-488 conjugated anti-mouse mAb (A11001, Life Technologies) was used to visualise staining. Plates were analysed using the ArrayScan VTi HCS Reader (NHBE) at 10× magnification, or the ImageXpress Micro XLS (CMT-64) at 20× magnification.
Scott I.C., Majithiya J.B., Sanden C., Thornton P., Sanders P.N., Moore T., Guscott M., Corkill D.J., Erjefält J.S, & Cohen E.S. (2018). Interleukin-33 is activated by allergen- and necrosis-associated proteolytic activities to regulate its alarmin activity during epithelial damage. Scientific Reports, 8, 3363.
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2

Isolation and Culture of Osteocytes

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Primary osteocyte-enriched fractions from Men1gtRosaCreERT2 mice were isolated as previously described.27 (link) Osteocytes were extracted by sequential digestions with collagenase and EDTA (AppliChem, Darmstadt, Germany). Fractions 3–5 of the sequential digest were considered as osteoblasts. Fractions 7–9 and cells derived from bone particles were pooled and considered as osteocytes. After one passage, 8000 cells per well were seeded into collagen-coated 96-well plates (Greiner Bio-One, Kremsmünster, Austria) and treated with 1 μM 4-OHT for 3 days.
Liu P., Lee S., Knoll J., Rauch A., Ostermay S., Luther J., Malkusch N., Lerner U.H., Zaiss M.M., Neven M., Wittig R., Rauner M., David J.P., Bertolino P., Zhang C.X, & Tuckermann J.P. (2017). Loss of menin in osteoblast lineage affects osteocyte–osteoclast crosstalk causing osteoporosis. Cell Death and Differentiation, 24(4), 672-682.
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