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Shfancd2.4 v3lhs 383035

Manufactured by Thermo Fisher Scientific
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The ShFancD2.4 (V3LHS_383035) is a laboratory equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

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Lab products found in correlation

Shrna, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (2 mentions)

2 protocols using shfancd2.4 v3lhs 383035

1

Efficient siRNA Transfection and shRNA Lentiviral Delivery

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For siRNA transfection cells were plated at a density of 50–80% on 6-cm dishes 1 d before transfection. siRNAs were purchased from Dharmacon and were used at a final concentration of 20 nM. Transfection was carried out using the Lipofectamine RNAiMAX reagent (Invitrogen) according to the manufacturer’s instructions. Lentiviral vector plasmids for stable, constitutive or inducible expression of secreted luciferases GLuc and CLuc coupled to p53 or nontargeting control shRNAs were described previously29 (link). shRNA sequences from Open Biosystems are as follows: shFancD2.2 (V2LHS_139155), shFancD2.3 (V3LHS_383034), shFancD2.4 (V3LHS_383035). siRNA sequences: siFancD2.6 CAACATACCTCGACTCATT, siFancD2.8 GGAGATTGATGGT CTACTA, simTOR.8 GGCCAUAGCUAGCCUCAUA, simTOR.9 CAAAGGA CUUCGCCCAUAA, simTOR.10:GCAGAAUUGUCAAGGGAUA, simTOR.11 CCAAAGCACUACACUACAA.
Wanzel M., Vischedyk J.B., Gittler M.P., Gremke N., Seiz J.R., Hefter M., Noack M., Savai R., Mernberger M., Charles J.P., Schneikert J., Bretz A.C., Nist A, & Stiewe T. (2015). CRISPR-Cas9-based target validation for p53-reactivating model compounds. Nature chemical biology, 12(1), 22-28.
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2

Efficient siRNA Transfection and shRNA Lentiviral Delivery

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For siRNA transfection cells were plated at a density of 50–80% on 6-cm dishes 1 d before transfection. siRNAs were purchased from Dharmacon and were used at a final concentration of 20 nM. Transfection was carried out using the Lipofectamine RNAiMAX reagent (Invitrogen) according to the manufacturer’s instructions. Lentiviral vector plasmids for stable, constitutive or inducible expression of secreted luciferases GLuc and CLuc coupled to p53 or nontargeting control shRNAs were described previously29 (link). shRNA sequences from Open Biosystems are as follows: shFancD2.2 (V2LHS_139155), shFancD2.3 (V3LHS_383034), shFancD2.4 (V3LHS_383035). siRNA sequences: siFancD2.6 CAACATACCTCGACTCATT, siFancD2.8 GGAGATTGATGGT CTACTA, simTOR.8 GGCCAUAGCUAGCCUCAUA, simTOR.9 CAAAGGA CUUCGCCCAUAA, simTOR.10:GCAGAAUUGUCAAGGGAUA, simTOR.11 CCAAAGCACUACACUACAA.
Wanzel M., Vischedyk J.B., Gittler M.P., Gremke N., Seiz J.R., Hefter M., Noack M., Savai R., Mernberger M., Charles J.P., Schneikert J., Bretz A.C., Nist A, & Stiewe T. (2015). CRISPR-Cas9-based target validation for p53-reactivating model compounds. Nature chemical biology, 12(1), 22-28.
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