Libra 120 plus ef tem transmission electron microscope

To fabricate the nanoparticles, a previously reported membrane coating approach was employed.^{37 (link)} First, poly(lactic-co-glycolic acid) (PLGA; carboxy-terminated, 50:50, 0.67 dL/g; Lactel Absorbable Polymers) cores were prepared by precipitating the polymer dissolved at 10 mg/mL using acetone into water, followed by evaporation to remove the organic solvent. Human RBC (hRBC) membrane ghosts were obtained by the hypotonic lysis of human O-negative RBCs (BioreclamationIVT). Human RBC membrane-derived vesicles were prepared by brief sonication of hRBC ghosts using a Fisher Scientific FS30D bath sonicator. To make hRBC-NPs, the membrane and PLGA cores were mixed together, followed by sonication to induce membrane fusion. Size and zeta potential were measured by dynamic light scattering (DLS) using a Malvern ZEN 3600 Zetasizer. To visualize the nanoparticles, the hRBC-NPs were deposited onto a 400-mesh carbon-coated copper grid (Electron Microscopy Sciences), stained with 1 wt% uranyl acetate (Electron Microscopy Sciences), and imaged using a Zeiss Libra 120 PLUS EF-TEM transmission electron microscope.