Cd206 ab64693

G418 was purchased from KSE Scientific (Durham, NC). Puromycin, DMEM, penicillin/streptomycin, gentamicin, DPBS, Hank’s Buffer, HEPES, EDTA, Tris base, sucrose, MgCl₂, Millipore filter plates and punch kits, EcoLite scintillation cocktail, and Poly-d-lysine coated 96-well plates were purchased from Fisher Scientific (Waltham, MA). Ambisome and FBS were purchased from Atlanta Biologicals (Flowery Branch, GA). Ro 20-1724, acetonitrile, DMSO, lipopolysaccharide (LPS), polyethyleneamine, and fatty acid-free BSA were purchased from Sigma Aldrich (St. Louis, MO). Antibodies against murine CD16/32 (ab33550) and CD206 (ab64693) were purchased from Abcam (Cambridge, UK). High bind plates (L15XB-3), anti-rat (R32AA-5), and anti-rabbit (R32AB-1) SULFO-TAG antibodies were purchased from Meso-Scale Discovery (Gaithersburg, MD). Cellular dyes phalloidin and Hoechst were purchased from Cellomics (Pittsburgh, PA), and ACTOne Membrane Potential Dye was purchased from Codex BioSolutions (Gaithersburg, MD). Forskolin, CP 55,940, HU 308, JWH 133, and SR 144528 were purchased from Tocris (Bristol, UK). Tritiated CP 55,940 was purchased from PerkinElmer (Waltham, MA). The 96-well filter plates and punch tips were purchased from Millipore (Billerica, MA).

Tendon-bone junction tissues were cut into pieces, and proteins were harvested from the tissues using ice-cold RIPA lysis. Then, the protein concentration was measured using a BCA Protein Assay kit (Beyotime, China). The protein samples were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes, which were blocked with 5% bovine serum albumin (BSA; Sangon Biotech, China) for 1 h. Later, primary antibody was added to incubate the membranes at 4°C overnight. Primary antibodies CD206 (ab64693, 1: 1000), CD81 (ab109201, 1: 500), CD9 (ab92726, 1: 800), and tumor susceptibility gene 101 (TSG101; ab125011, 1: 1000) were purchased from Abcam, and Arg1 (93668S, 1: 1000) was purchased from Cell Signaling Technology. Then, the membranes were incubated with HRP-conjugated secondary antibody (Beyotime, A0208, 1: 1000) at 37°C for 2 h. Finally, the reaction solution was added for exposure analysis.