Cell apoptosis was measured using PE Annexin V Kit I (BD Biosciences, NJ, USA). Briefly, cells were collected and resuspended in 1× binding buffer. Then, the solution (1 × 105 cells) was supplemented with 5 μL of PE Annexin V and 7-AAD and incubated in the dark for 15 min at room temperature. Apoptotic cells were identified by flow cytometry (Beckman Coulter, CA, USA). All the experiments were performed in triplicate.
Pe annexin 5 kit 1
Manufactured by BD
Sourced in United States
The PE Annexin V Kit I is a laboratory product designed for the detection and quantification of apoptosis in cells. It contains Annexin V conjugated with the fluorescent dye Phycoerythrin (PE) for the visualization of phosphatidylserine exposure, a hallmark of apoptotic cells.
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Lab products found in correlation
4 protocols using pe annexin 5 kit 1
1
Quantifying Cell Apoptosis by Flow Cytometry
2
Quantifying Apoptosis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell apoptosis was measured using PE Annexin V Kit I (BD Biosciences, NJ, USA). Briefly, cells were collected and resuspended in 1× binding buffer. Thereafter, the solution (1×10 5 cells) supplemented with 5 μL of PE Annexin V and 7-AAD was incubated in the dark for 15 min at room temperature. The apoptotic cells were identied by ow cytometry (Beckman Coulter, CA, USA). All the experiments were performed in triplicate.
3
Quantifying Apoptosis via Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis was measured using PE Annexin V Kit I (BD Biosciences, NJ, USA). Brie y, cells were collected and resuspended in 1× binding buffer. Then, the solution (1×10 5 cells) was supplemented with 5 μL of PE Annexin V and 7-AAD and incubated in the dark for 15 min at room temperature. Apoptotic cells were identified by flow cytometry (Beckman Coulter, CA, USA). All the experiments were performed in triplicate.
4
Apoptosis Measurement by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis was measured using the PE Annexin V Kit I (BD Biosciences, USA). Brie y, cells were collected and resuspended in 1 × binding buffer. Thereafter, the solution (1 × 10 5 cells) supplemented with 5 µL PE Annexin V and 7-AAD were incubated in the dark for 15 min at room temperature. The apoptotic cells were identi ed by ow cytometry (Beckman Coulter, USA). All the experiments were performed in triplicate.
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