Sc 29827

siRNAs targeting human 53BP1 (sc-37455) and BRG1 (sc-29827) and TOP2A (sc-36695) were obtained from Santa Cruz Biotechnology. siRNA pools targeting MDC1 (L-003506-00) and PICH (L-031581-01) were obtained from Dharmacon. Oligonucleotide mix targeting the 3′ UTR of endogenous human TOPBP1 (Dharmacon) contained the following oligos: 5′-GUAAAUAUCUGAAGCUGUAUU-3′, 5′-GCACAAGGUUUAAUGAGGAUU-3′, 5′-GCUGUAGCUUAGUGGAAAUUU-3′. Oligonucleotide targeting the 3′ UTR of BLM (Dharmacon) was as follows: 5′-GCUAGGAGUCUGCGUGCGAUU-3′. siRNA targeting TOP2B was 5′-UCGGGCUAGGAAAGAAGUAAA-3′^{50 (link)}:. Santa Cruz Biotechnology non-targeting siRNA control (sc-37007) or Dharmacon non-targeting smart pool siRNA (D-00180-10-20) were used as control siRNAs where appropriate. Oligonucleotides were transfected into human cells using Oligofectamine reagent (Life Technologies), according to the manufacturer’s instructions.