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2 protocols using acrylic acid

1

Synthesis of Polymerized PEG-Conjugates

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Acrylic acid (AA, 99.5%) stabilized with 200 ppm 4-methoxyphenol, methanol (99.8%), and sodium borate buffer were obtained from VWR (Radnor, PA, USA). 4,4′-Azobis-(4-cyanopentanoic acid) (A-CPA) and 4-cyano-4-(phenylcarbonothioylthio)pentanoic acid (CPA-DB) (>97% HPLC) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Methoxy-poly(ethylene glycol)-amine powder (PEG-NH2) was obtained from Jenkem Technologies (Beijing, China) and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was obtained from TCI America (Portland, OR, USA). All chemicals were used as received unless otherwise specified.
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2

Membrane Filtration of Chemical Solutions

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Methanol, ethanol, benzophenone (BP), MgSO4, NaCl, MgCl2, NaSO4, acrylic acid (AA), and phosphate-buffered saline (PBS) tablets were purchased from VWR (Atlanta, GA, USA). Bovine serum albumin (BSA) was supplied by Lee BioSolution (Maryland Heights, MO, USA). Congo red and Eriochrome® Black T(EBT) were procured from Sigma-Aldrich (St. Louis, MO, USA). DI water was used in all experiments. Commercial polyvinylidene fluoride (PVDF) 400kDa membrane was provided by the UltraTM, Oceanside, CA. Commercial nanofiltration membrane NF270, NF90, and NF245 were provided by DuPont Water Solutions (Midland, MI, USA).
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