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Anti cd28 ecd clone cd28.2

Manufactured by Beckman Coulter
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Anti-CD28-ECD (clone CD28.2) is a monoclonal antibody that binds to the CD28 receptor on the surface of T cells. It is conjugated with the Electron Coupled Dye (ECD) fluorochrome.

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Lab products found in correlation

Aqua live dead amine dye amcyan, by Thermo Fisher Scientific (3 mentions) Anti cd8 qdot705 clone 3b5, by Thermo Fisher Scientific (3 mentions) Anti cxcr3 alexafluor 488 clone 1c6 cxcr3, by BD (2 mentions) Anti cd161 pe clone hp 3g10 and anti il 17 alexa488 clone ebio64dec17, by Thermo Fisher Scientific (2 mentions) Anti cd161 bv421 clone hp 3g10, by BioLegend (2 mentions) Prism version 6, by GraphPad (1 mentions) Cytofix cytoperm, by BD (1 mentions)

Close spelling variants of this product

CD28-ECD (clone CD28-2) CD28 (CD28.2) ECD CD28-ECD (CD28-2) CD28-ECD Anti-CD28 [CD28.2] CD28 (clone CD28.2, Clone CD28.2 CD28 (CD28.2) Anti-CD28

3 protocols using anti cd28 ecd clone cd28.2

1

Comprehensive Immune Profiling by Flow Cytometry

Cited 1 time
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Fourteen-parameter flow cytometric analysis was performed on peripheral blood, LN, and RB derived cells according to standard procedures using a panel of monoclonal antibodies that we and others have shown to be cross-reactive with SMs and RMs.12 (link) The following antibodies were used at predetermined optimal concentrations: anti-CCR6-PE-Cy7 (clone 11A9), anti-CCR5-PE and -APC (clone 3A9), anti-CD3-APC-Cy7 (clone SP34-2), anti-CD62L-FITC (clone SK11), anti-CD95-PE-Cy5 (clone DX2), anti-Ki-67-Alexa700 (clone B56), anti-IFN-γ-Alexa700 (clone B27), and anti-CXCR3-AlexaFluor 488 (clone 1C6/CXCR3) from BD Biosciences; anti-CD161-PE (clone HP-3G10) and anti-IL-17-Alexa488 (clone eBio64DEC17) from eBioscience; anti-CD28-ECD (clone CD28.2) from Beckman Coulter; anti-CD4-BV421 (clone OKT4), anti-CD4-BV605 (clone OKT4), and anti-CD161-BV421 (clone HP-3G10) from Biolegend; anti-CD8-Qdot705 (clone 3B5) and Aqua Live/Dead amine dye-AmCyan from Invitrogen. Flow cytometric acquisition was performed on at least 100,000 CD3+ T cells on an LSRII cytometer driven by the FACS DiVa software, or at least 10,000 CD3+ T cells for rectal biopsy-derived cells. The data acquired were analyzed using FlowJo software (version 9.8.5; TreeStar).
McGary C.S., Alvarez X., Harrington S., Cervasi B., Ryan E.S., Iriele R.I., Paganini S., Harper J., Easley K., Silvestri G., Ansari A.A., Lichterfeld M., Micci L, & Paiardini M. (2017). The loss of CCR6+ and CD161+ CD4+ T cell homeostasis contributes to disease progression in SIV-infected rhesus macaques. Mucosal immunology, 10(4), 1082-1096.
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2

Comprehensive Immune Profiling by Flow Cytometry

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fourteen-parameter flow cytometric analysis was performed on peripheral blood, LN, and RB derived cells according to standard procedures using a panel of monoclonal antibodies that we and others have shown to be cross-reactive with SMs and RMs.12 (link) The following antibodies were used at predetermined optimal concentrations: anti-CCR6-PE-Cy7 (clone 11A9), anti-CCR5-PE and -APC (clone 3A9), anti-CD3-APC-Cy7 (clone SP34-2), anti-CD62L-FITC (clone SK11), anti-CD95-PE-Cy5 (clone DX2), anti-Ki-67-Alexa700 (clone B56), anti-IFN-γ-Alexa700 (clone B27), and anti-CXCR3-AlexaFluor 488 (clone 1C6/CXCR3) from BD Biosciences; anti-CD161-PE (clone HP-3G10) and anti-IL-17-Alexa488 (clone eBio64DEC17) from eBioscience; anti-CD28-ECD (clone CD28.2) from Beckman Coulter; anti-CD4-BV421 (clone OKT4), anti-CD4-BV605 (clone OKT4), and anti-CD161-BV421 (clone HP-3G10) from Biolegend; anti-CD8-Qdot705 (clone 3B5) and Aqua Live/Dead amine dye-AmCyan from Invitrogen. Flow cytometric acquisition was performed on at least 100,000 CD3+ T cells on an LSRII cytometer driven by the FACS DiVa software, or at least 10,000 CD3+ T cells for rectal biopsy-derived cells. The data acquired were analyzed using FlowJo software (version 9.8.5; TreeStar).
McGary C.S., Alvarez X., Harrington S., Cervasi B., Ryan E.S., Iriele R.I., Paganini S., Harper J., Easley K., Silvestri G., Ansari A.A., Lichterfeld M., Micci L, & Paiardini M. (2017). The loss of CCR6+ and CD161+ CD4+ T cell homeostasis contributes to disease progression in SIV-infected rhesus macaques. Mucosal immunology, 10(4), 1082-1096.
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3

Twelve-parameter Flow Cytometry Analysis

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Twelve-parameter flow cytometric analysis was performed on WB, LN, RB and BM derived cells according to standard procedures using a panel of monoclonal antibodies that we and others have shown to be cross-reactive with RM [35] (link)–[37] (link). Predetermined optimal concentrations were used of the following antibodies: anti-CD3-Alexa700 (clone SP34-2), anti-CD3-APC-Cy7 (clone SP34-2), anti-CD4-Pacific Blue (clone OKT4), anti-CD8-APC-Cy7 (clone SK1), anti-CD95-PE-Cy5 (clone DX2), anti-CCR5-APC (clone 3A9), anti-Ki-67-Alexa700 (clone B56), anti-Ki-67-FITC (clone B56), anti-CD14-Pe-Cy7 (clone M5E2), anti-CD16-BV421 (clone 3G8), anti-CD62L-PE (clone SK11), anti-CCR7-PE-Cy7 (Clone 3D12) (all from BD Pharmingen); anti-CD28-ECD (clone CD28.2) (Beckman Coulter); anti-CD8-Qdot705 (clone 3B5) and Aqua Live/Dead amine dye-AmCyan (Invitrogen). Intracellular staining for Ki-67 was performed at room temperature for 30 minutes following permeabilization with cytofix/cytoperm (BD Bioscience). Flow cytometric acquisition was performed on an LSRII cytometer driven by the FACS DiVa software. Analysis of the acquired data was performed using FlowJo software (TreeStar) and graphs were prepared using Prism version 6.0 (GraphPad).
Micci L., Alvarez X., Iriele R.I., Ortiz A.M., Ryan E.S., McGary C.S., Deleage C., McAtee B.B., He T., Apetrei C., Easley K., Pahwa S., Collman R.G., Derdeyn C.A., Davenport M.P., Estes J.D., Silvestri G., Lackner A.A, & Paiardini M. (2014). CD4 Depletion in SIV-Infected Macaques Results in Macrophage and Microglia Infection with Rapid Turnover of Infected Cells. PLoS Pathogens, 10(10), e1004467.
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