Ampicillin

Manufactured by Sinopharm

Sourced in China, Hong Kong

Ampicillin is a laboratory-grade antibiotic powder used in microbiology and biotechnology research. It inhibits the synthesis of bacterial cell walls, thereby exerting its antimicrobial effect.

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Lab products found in correlation

4 protocols using ampicillin

Diverse Biological Reagents Acquisition

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Neutral protease, papain, chitosan, bovine insulin, myoglobin, and tryptophan were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Tryptone, yeast extract, Agar, NaCl, glycerin, K₂HPO₄·3H₂O (AR), sorbitol, MgSO₄·7H₂O (AR), trisodium citrate, MgCl₂ (AR), kanamycin, ampicillin, peptone, maltose, sodium acetate, calcium sulfate, acetic acid, Triton X-100, phosphate-buffered saline, normal saline, trichloroacetic acid, and other reagents (AR) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Tian M., Ning C., Peng S., Li D., Jin R., Zhang Y., Liu Z., Mou H, & Zhu C. (2023). High-Efficiency Fermentation of Nattokinase by Recombinant PSP2 Using Oyster Protein Hydrolysate as a Substrate. Foods, 12(6), 1252.

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Rapid Immunoassay for Tetracycline Detection

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Trimethoxyoctadecylsilane (C₂₁H₄₆O₃Si), bovine serum albumin (BSA) and tetracycline hydrochloride (C₂₂H₂₄N₂O₈·HCl) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ampicillin, penicillin, streptomycin, kanamycin, dipotassium phosphate (K₂HPO₄), monopotassium phosphate (KH₂PO₄), sodium chloride (NaCl) and magnesium chloride (MgCl₂) were purchased from Sinopharm (Hong Kong, China). Tween-20 was purchased from Solarbio (Beijing, China). The filter paper was purchased from Whatman (Maidstone, UK). Tetracycline-BSA antigen and anti-tetracycline monoclonal antibody were purchased from Suzhou Kuaijiekang Co., Ltd. (Suzhou, China). Commercial tetracycline ELISA kit was purchased from Shenzhen Finder Biotech Co., Ltd. (Shenzhen, China). The heating plate (YH-946B) was purchased from Shanghai Yuxing Electronics Co., Ltd. (Shanghai, China). The inkjet printer (IP2780) was purchased from Canon (Tokyo, Japan).

Li J., Wang X., Shan Y., Huang H., Jian D., Xue L., Wang S, & Liu F. (2019). Handheld Inkjet Printing Paper Chip Based Smart Tetracycline Detector. Micromachines, 10(1), 27.

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Recombinant Protein Expression Protocols

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Restriction endonuclease BamHI, NheI, SpeI, and T4 DNA ligase were obtained from New England Biolabs Inc. (Beijing, China). Ampicillin and kanamycin were purchased from Sinopharm Group Chemical Reagent Co., Ltd (Shanghai, China). Isopropyl-β-d-thiogalactoside (IPTG) and nickelnitrilotriacetic acid (Ni-NTA) separation column were purchased from Qiagen Co., Ltd (Shanghai, China). Cobalt chloride hexahydrate (99.99%), gold chloride trihydrate (HAuCl₄·3H₂O), trisodium citrate dehydrate (> 99%), polyvinylpyrrolidone (PVP, MW: 55,000), sodium borohydride (99%), calcein AM, Propidium Iodide (PI), and Doxorubicin Hydrochloride (DOX) were obtained from Sigma-Aldrich, Inc. (USA). Ultrapure water (18.2 MΩ) purified by the Milli-Q system (Millipore, DE) was used to prepare all solutions.

Jin R., Yang J., Zhao D., Hou X., Li C., Chen W., Zhao Y., Yin Z, & Liu B. (2019). Hollow gold nanoshells-incorporated injectable genetically engineered hydrogel for sustained chemo-photothermal therapy of tumor. Journal of Nanobiotechnology, 17, 99.

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Multifunctional Theranostic Nanoparticles

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NheI and SpeI were obtained from New England Biolabs. Nickel nitrilotiacetic acid resin was obtained from Qiagen. 1-dodecanethiol (DT, 98%), diethyldithiocarbamic acid silver salt (98%), trichlormethane, acetone, dimethyl sulfoxide (DMSO), ampicillin, and kanamycin were purchased from Sinopharm Group Chemical Reagent Co. Ltd. Chlorin e6 (Ce6) was obtained from Frontier Scientific Inc., and 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[amine methoxy (polyethylene glycol)₂₀₀₀] (DSPE-mPEG₂₀₀₀-NH₂) was purchased from Avanti. Ultrapure water (Millipore Mill-Q grade, 18.2 MΩ) was used in all the experiments. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) were obtained from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). DMEM/F12 and RPMI 1640 were purchased from Thermo Fisher Scientific. Staphylococcus aureus’ species-identifiable aptamer was obtained from Tsingke Biotechnology Co., Ltd. All reagents and solvents are of analytical grade and used without further purification.

Xie X., Wei J., Zhang B., Xiong W., He Z., Zhang Y., Gao C., Zhao Y, & Liu B. (2022). A self-assembled bilayer polypeptide-engineered hydrogel for spatiotemporal modulation of bactericidal and anti-inflammation process in osteomyelitis treatment. Journal of Nanobiotechnology, 20, 416.

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