8- to 12-week-old male and female C57BL/6 mice were anesthetized with a mixture of xylazine/ketamine (12/100mg per kg) and kept anesthetized for the duration of the experiment with subsequent ketamine doses (50mg/kg of weight) as needed. A catheter was inserted into the carotid artery so that the infusion went straight to the brain rather than towards the thoracic cavity. An area of the skull was cleared of tissue and thinned to expose the branching arteries originating from the MCA on the side the catheter was inserted, above the zygomatic arch, between the ear and eye of the skull (Busija and Leffler, 1991 (link)). The exposed arteries branching out from the MCA were monitored using a Leica MC170 HD microscope with a mounted camera (Leica M125 C) connected to a computer monitor. Drugs were diluted to their final concentration in sodium saline (0.9% NaCl) and administered via catheter at 0.1 mL/25 g of weight. Cranial window images before and after drug administration were acquired every 60 seconds for subsequent analysis.
Mc170 hd microscope
Manufactured by Leica
The Leica MC170 HD is a high-definition microscope designed for laboratory applications. It features a 3.2-megapixel CMOS sensor that captures detailed images and video in 1080p resolution. The microscope offers adjustable magnification and can be connected to a computer or display for image analysis and documentation.
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Lab products found in correlation
7 protocols using mc170 hd microscope
1
Cranial Window Imaging of Mouse Cerebral Arteries
2
Microscopic Imaging for Specimen Analysis
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A Leica MC170HD microscope camera, mounted on a Leica M205C stereomicroscope, was used to capture images of whole specimens and of detached terminalia macerated in hot lactic acid and stored in glycerine. Stacked images, merged for extended focus applying the Helicon Focus software, were subsequently moderately photo-shopped into illustrative plates. Digital illustrations (Fig. 10) were made with Inkscape vector drawing editor (http://inkscape.org).
3
Measuring Cerebral Artery Responses
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Male and female C57BL/6J mice, all 8–12 weeks old, were deeply anesthetized with isoflurane via inhalation using an open-drop method in a bell jar. Upon losing their response to toe pinch, animals were quickly decapitated with sharp scissors. Resistance-size MCAs (~100 μm in outer diameter) were dissected from the mouse brains. Endothelium was removed by passing an air bubble through the vessel lumen for 90 s [29 (link)]. Arterial segments (0.5 cm long) were cannulated at each end, and the artery exterior was continuously perfused with physiologic sodium saline (PSS) of the following composition (mM): 119 NaCl, 4.7 KCl, 1.2 KH2PO4, 1.6 CaCl2, 1.2 MgSO4, 0.023 EDTA, 11 glucose, and 24 NaHCO3; pH = 7.4, at 35°C–37°C. PSS was continuously bubbled with O2/CO2/N2 at 21/5/74%. Vehicle control (dimethyl sulfoxide; DMSO), PREG, EtOH, or the PREG + EtOH combination were diluted into PSS and perfused over the arterial segment. The artery external wall diameter was measured using the automatic edge-detection function of the IonWizard software package (IonOptix) via a Leica MC170 HD microscope with a mounted camera (Leica M125 C) connected to a computer monitor.
4
Monitoring Cerebral Arteries in Mice
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C57BL/6J mice of both sexes, all 8–12 weeks old, were anesthetized with a mixture of xylazine/ketamine (12/100 mg/kg of weight) and kept anesthetized for the duration of the experiment with subsequent ketamine doses (50 mg/kg of weight) every 15 min or as needed. A catheter was inserted into the internal carotid artery so that experimental drug infusions were directed toward the brain rather than the thoracic cavity. An area of the skull was cleared of tissue and thinned in order to visualize the branching arteries originating from the middle cerebral artery (MCA) on the brain side where the catheter was inserted, above the zygomatic arch, between the ear and eye [27 (link), 40 (link)]. The arteries branching out from the MCA were monitored using a Leica MC170 HD microscope with a mounted camera (Leica M125 C) connected to a computer monitor. Drugs were diluted to their final concentration in 0.9% NaCl and administered via catheter at 0.1 mL/25 g of mouse weight. Cranial window images before and after drug administration were acquired every 60 s for later analysis; a sample of n = 5–6 was acquired for each group (with n representing the number of separate animals).
5
Monitoring Cerebral Arteries in Mice
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C57BL/6J mice of both sexes, all 8–12 weeks old, were anesthetized with a mixture of xylazine/ketamine (12/100 mg/kg of weight) and kept anesthetized for the duration of the experiment with subsequent ketamine doses (50 mg/kg of weight) every 15 min or as needed. A catheter was inserted into the internal carotid artery so that experimental drug infusions were directed toward the brain rather than the thoracic cavity. An area of the skull was cleared of tissue and thinned in order to visualize the branching arteries originating from the middle cerebral artery (MCA) on the brain side where the catheter was inserted, above the zygomatic arch, between the ear and eye [27 (link), 40 (link)]. The arteries branching out from the MCA were monitored using a Leica MC170 HD microscope with a mounted camera (Leica M125 C) connected to a computer monitor. Drugs were diluted to their final concentration in 0.9% NaCl and administered via catheter at 0.1 mL/25 g of mouse weight. Cranial window images before and after drug administration were acquired every 60 s for later analysis; a sample of n = 5–6 was acquired for each group (with n representing the number of separate animals).
6
Measuring Cerebral Artery Responses
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Male and female C57BL/6J mice, all 8–12 weeks old, were deeply anesthetized with isoflurane via inhalation using an open-drop method in a bell jar. Upon losing their response to toe pinch, animals were quickly decapitated with sharp scissors. Resistance-size MCAs (~100 μm in outer diameter) were dissected from the mouse brains. Endothelium was removed by passing an air bubble through the vessel lumen for 90 s [29 (link)]. Arterial segments (0.5 cm long) were cannulated at each end, and the artery exterior was continuously perfused with physiologic sodium saline (PSS) of the following composition (mM): 119 NaCl, 4.7 KCl, 1.2 KH2PO4, 1.6 CaCl2, 1.2 MgSO4, 0.023 EDTA, 11 glucose, and 24 NaHCO3; pH = 7.4, at 35°C–37°C. PSS was continuously bubbled with O2/CO2/N2 at 21/5/74%. Vehicle control (dimethyl sulfoxide; DMSO), PREG, EtOH, or the PREG + EtOH combination were diluted into PSS and perfused over the arterial segment. The artery external wall diameter was measured using the automatic edge-detection function of the IonWizard software package (IonOptix) via a Leica MC170 HD microscope with a mounted camera (Leica M125 C) connected to a computer monitor.
7
Petiole and Leaf Blade Anatomy: Paraffin Sectioning
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The middle portions of petiole and mature leaf blades which were leaf margin, midrib and the region between leaf margin and midrib were dehydrated in series of TBA (tertiary butyl alcohol). The specimens were infiltrated by paraffin liquid and pure paraffin before embedded into molds. A thin section of 12-14 µm was made using a sliding microtome and stained with safranin O and fast green, ahead of mounting with DePeX mounting medium (Ravichandra 2010) . The replications of ten leaves were also conducted in each leaf area of paraffin technique. Wood sectioning Preparation of sections for light microscopy was made by maintaining of stem from adult trees at 1.5-meter height. Then, the wood was cut and trimmed as a cubic in 1x1x1 cm 3 . Wood sectioning of 10-14 µm was prepared using a sliding microtome in three surfaces including transverse, tangential and radial sections. The tissues were stained with safranin O and dehydrated in series of alcohol before mounting with DePeX mounting medium. For terminology and determinations of the quantitative and qualitative features of the wood, the IAWA Hardwood List followed Wheeler et al. (1989) . Minimum of 50 measurements for diameter on ten samples of each species were performed.
Eventually, the permanent slides were analyzed and photographed using a LEICA MC170HD microscope. The mensuration of vessel was diagnosed by ImageJ program.
Eventually, the permanent slides were analyzed and photographed using a LEICA MC170HD microscope. The mensuration of vessel was diagnosed by ImageJ program.
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