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Prepacked disposable pd 10 columns

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Prepacked disposable PD-10 columns are a type of lab equipment used for size-exclusion chromatography. These columns are pre-filled with a gel-filtration medium and are designed for single-use applications. They are commonly used for buffer exchange, desalting, and sample cleanup procedures.

Automatically generated - may contain errors

Lab products found in correlation

Pierce centrifuge column, by Thermo Fisher Scientific (2 mentions) Simply blue life stain, by Thermo Fisher Scientific (2 mentions) Hispur cobalt resin, by Thermo Fisher Scientific (2 mentions) Expi293 cell, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PD-10 column (853 citations) PD-10 (119 citations) PD-10 disposable column (6 citations) Prepacked columns (4 citations)

2 protocols using prepacked disposable pd 10 columns

1

TriKE Protein Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
TriKE plasmids were transfected into Expi293 cells (Thermo Fisher, Waltham, MA) according to the manufacturer’s protocol and protein was purified using HisPur Cobalt Resin (Thermo Fisher) and Pierce centrifuge columns (Thermo Fisher). Protein was eluted using 250mM imidazole solution and desalted using Prepacked disposable PD-10 columns (GE Healthcare, Chicago, IL). Purity and size (57KDa) were determined by running sodium dodecyl sulfate polyacrylamide gel electrophoresis using Simply Blue Life Stain (Invitrogen, Carlsbad, CA).
Arvindam U.S., van Hauten P.M., Schirm D., Schaap N., Hobo W., Blazar B.R., Vallera D.A., Dolstra H., Felices M, & Miller J.S. (2020). A trispecific killer engager molecule against CLEC12A effectively induces NK cell mediated killing of AML cells. Leukemia, 35(6), 1586-1596.
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2

TriKE Protein Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
TriKE plasmids were transfected into Expi293 cells (Thermo Fisher, Waltham, MA) according to the manufacturer’s protocol and protein was purified using HisPur Cobalt Resin (Thermo Fisher) and Pierce centrifuge columns (Thermo Fisher). Protein was eluted using 250mM imidazole solution and desalted using Prepacked disposable PD-10 columns (GE Healthcare, Chicago, IL). Purity and size (57KDa) were determined by running sodium dodecyl sulfate polyacrylamide gel electrophoresis using Simply Blue Life Stain (Invitrogen, Carlsbad, CA).
Arvindam U.S., van Hauten P.M., Schirm D., Schaap N., Hobo W., Blazar B.R., Vallera D.A., Dolstra H., Felices M, & Miller J.S. (2020). A trispecific killer engager molecule against CLEC12A effectively induces NK cell mediated killing of AML cells. Leukemia, 35(6), 1586-1596.
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