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2 protocols using plastic pipette tips

1

Lipid Extraction and Quantification Protocol

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All solvents were LC-MS grade. Water and isopropyl alcohol were purchased from Honeywell Burdick & Jackson (Muskegon, Michigan); chloroform, acetonitrile, methanol, acetic acid, low retention microcentrifuge tubes, serological pipettes were purchased from Fisher Scientific (Fair Lawn, New Jersey); plastic pipette tips were purchased from USA Scientific (Orlando, Florida); methyl tert-butyl ether was purchased from J.T. Baker (Central City, Pennsylvania); internal standards were purchased from Avanti Polar lipids Inc. and Sigma Aldrich (St. Louis, MO); pyrex glass culture tubes were purchased from Corning Incorporated (Corning, New York).
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2

Analytical Lipid Extraction Workflow

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All solvents were LC-MS grade. Water and isopropyl alcohol were purchased from Honeywell Burdick & Jackson (Muskegon, MI, USA); chloroform, acetonitrile, methanol, acetic acid, low retention microcentrifuge tubes, serological pipettes were purchased from Fisher Scientific (Fair Lawn, NJ, USA); plastic pipette tips were purchased from USA Scientific (Orlando, FL, USA); methyl tert-butyl ether was purchased from J.T. Baker (Central City, PA, USA); internal standards were purchased from Avanti Polar lipids Inc. and Sigma Aldrich (St. Louis, MO, USA); pyrex glass culture tubes were purchased from Corning Incorporated (Corning, NY, USA).
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