Log In Sign up
The largest database of trusted experimental protocols

Human 48 plex luminex procarta immuno assay

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Human 48-plex Luminex Procarta Immuno-assay is a multiplexed assay that allows for the simultaneous detection and quantification of 48 different human analytes in a single sample. The assay utilizes Luminex xMAP technology to measure multiple biomarkers in a single well.

Automatically generated - may contain errors

Lab products found in correlation

Fluorescence activated cell sorting (facs), by Sony (2 mentions)

Spelling variants of this product

Luminex assay (34 citations) Luminex Human assay (2 citations)

2 protocols using human 48 plex luminex procarta immuno assay

1

APOE4 Aβ Fibrils Lipid Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
APOE4/4 iMG were treated with 5 μM Aβ fibrils stained with LipidSpot 488 as described above then top 10% highest and 10% lowest LipidSpot fluorescent cells were seeded into 96-well plates at 5,000 cells per well by FACS (Sony, MA900) in 100 μl of MGdM media for 24 h. Media supernatant was collected and secreted signalling proteins were measured in culture supernatants by the Human Immune Monitoring Center at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (Thermo Fisher).
Haney M.S., Pálovics R., Munson C.N., Long C., Johansson P.K., Yip O., Dong W., Rawat E., West E., Schlachetzki J.C., Tsai A., Guldner I.H., Lamichhane B.S., Smith A., Schaum N., Calcuttawala K., Shin A., Wang Y.H., Wang C., Koutsodendris N., Serrano G.E., Beach T.G., Reiman E.M., Glass C.K., Abu-Remaileh M., Enejder A., Huang Y, & Wyss-Coray T. (2024). APOE4/4 is linked to damaging lipid droplets in Alzheimer’s disease microglia. Nature, 628(8006), 154-161.
+ Open protocol
+ Expand
2

Aβ Fibrils Induce Immune Responses in APOE4/4 iMG

Check if the same lab product or an alternative is used in the 5 most similar protocols
APOE4/4 iMG were treated with 5 μM Aβ fibrils stained with Lipidspot 488 as described above then top 10% highest and 10% lowest Lipidspot fluorescent cells were seeded into 96-well plates at 5,000 cells per well by FACS (Sony, MA900) in 100 μl MGdM media for 24 h. Media supernatant was collected, and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
Haney M.S., Pálovics R., Munson C.N., Long C., Johansson P., Yip O., Dong W., Rawat E., West E., Schlachetzki J.C., Tsai A., Guldner I.H., Lamichhane B.S., Smith A., Schaum N., Calcuttawala K., Shin A., Wang Y.H., Wang C., Koutsodendris N., Serrano G.E., Beach T.G., Reiman E.M., Glass C.K., Abu-Remaileh M., Enejder A., Huang Y, & Wyss-Coray T. (2023). APOE4/4 is linked to damaging lipid droplets in Alzheimer’s microglia. bioRxiv.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!