Hoechst 33342

High glucose Dulbecco’s Modified Eagle Medium (DMEM), trypsin and penicillin–streptomycin, were obtained from Macgene (Beijing, China). Fetal bovine serum (FBS) was from PAN-Biotech (Aidenbach, Germany). Complete/Incomplete Freund’s adjuvant and LPS were from Sigma-Aldrich (St Louis, MO, USA). Bovine type II collagen was from Macklin (Shanghai, China). Methotrexate was obtained from Bidepharm (Shanghai, China). 4% paraformaldehyde was from Bioroyee Biotechnology (Beijing, China). EDTA and DAPI were from Solarbio (Beijing, China). Goat anti-rabbit secondary antibody, endogenous peroxidases blocker and goat serum albumin were from ZSGB-Biotech ((Beijing, China). PMA are purchased from Med Chem Express (MCE). Hoechst 33342 was obtained from TargetMol (Shanghai, China). Sytox Green was from Beyotime (Shanghai, China). Primary antibody of IL-6 was from Bioss (Beijing, China). Primary antibody of TNF-α was from Proteintech (Chicago, IL, USA). Primary antibodies against PI3K/p-PI3K were from Bioworld (St. Louis Park, MN, USA). Primary antibodies against p-Akt and p-mTOR were bought from Cell Signaling Technology (Beverly, MA, USA). Primary antibody against Akt was from Biogot (Nanjing, China). Antibodies of mTOR, MPO, CitH3 were from Abcam (Cambridge, UK).