Isomers of αA51–60 and αB61–67 composed of L-α- and D-β-Asp residues were synthesized by Fmoc solid-phase chemistry using an automated solid-phase peptide synthesizer (Shimadzu PSSM-8). Fmoc-amino acids from Watanabe Chemical Industries (Hiroshima, Japan) were used. Crude peptides were purified by RP-HPLC using a C18 column (Capcellpak C18 ACR, 10 × 250 mm; Shiseido) with a linear gradient of 10–60% (for αA51–60) and 5–55% acetonitrile (for αB61–67) for 60 min in the presence of 0.1% TFA at a flow rate of 3.0 mL/min with monitoring at 215 nm. HPLC grade solvent was used to confirm the purity of the peptide. The purity of each peptide was confirmed to be >98% by RP-HPLC and MALDI-TOF MS or ESI-MS. The masses ([M+H]+) observed for the protonated precursor ions of αA51–60 and αB61–67 were 1094.6 and 696.1. These values were consistent with the theoretical ones, 1094.58 and 696.32, respectively.
Capcellpak c18 acr
Manufactured by Shiseido
Sourced in Japan
The Capcellpak C18 ACR is a laboratory instrument used for high-performance liquid chromatography (HPLC) analysis. It is designed to separate, identify, and quantify the components within a mixture. The Capcellpak C18 ACR utilizes a packed column filled with chemically modified silica particles to facilitate the separation process.
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Lab products found in correlation
4 protocols using capcellpak c18 acr
1
Synthesis and Characterization of Isomeric Peptides
2
Synthetic Fibrinopeptide B Purification
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Fibrinopeptide B (1QGVNDNEEGFFSAR14) was synthesized by 9-fluorenylmethyloxycarbonyl group (Fmoc)-based solid-phase peptide synthesis using an automated solid-phase peptide synthesizer (PSSM-8; Shimadzu, Japan). The coupling reaction was carried out by mixing each Fmoc amino acid (10 eq), (benzotriazol-1-yloxy)-tripyrrolidinophosphonium hexafluorophosphate (10 eq), 1-hydroxybenzotriazole hydrate (10 eq), and N-methylmorpholine (7.5 eq) in N,N-dimethylformamide (DMF). The N-terminal Fmoc group was deblocked with 30% piperidine in DMF. Spontaneous cleavage of the peptide from the resin and removal of the protective groups were achieved by treatment with a mixture containing 82.5% TFA, 5% water, 5% thioanisole, 3% ethylmethylsulfide, 2.5% 1,2-ethanedithiol, and 2% thiophenol for 6 h. The crude peptides were purified by reversed-phase high-performance liquid chromatography using a C18 column (Capcell Pak C18 ACR, 10 × 250 mm2; Shiseido, Japan) with a linear gradient of 0%−50% acetonitrile in the presence of 0.1% TFA at a flow rate of 3.0 mL/min and detection at 230 nm.
3
Pharmacokinetic Study of DAP Cocrystal
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The PK test in beagle dogs was performed at the Chemon Non-Clinical Research Institute (Gyeonggi-do, Korea) and approved by the Ethics Committee (Protocol number: 18-D304). Twelve beagle dogs were kept on an empty stomach overnight, followed by administration of a DAP cocrystal tablet and Forxiga® 10 mg to each of the six beagle dogs. After the first dose, the drug was washed out for 7 days, and the second phase was cross-dose. Water intake was discontinued 2 h before drug administration, and all beagle dogs were supplied with water and food 4 h after the first dose. Blood samples were collected at predetermined time points (0.25, 0.50, 0.75, 1, 1.5, 2, 3, 4, 6, 8, and 24 h). All blood samples were centrifuged at 3000× g for 10 min, and aliquots of plasma were stored at −80 °C until analyzed using the validated LC-MS/MS method. Plasma concentration of DAP was determined by LC–MS/MS (Agilent 1260; Agilent Technologies Inc., Santa Clara, CA, USA). Chromatographic separation of DAP was performed using an ODS column (Capcellpak C18 ACR, 4.6 × 150 mm, 5 mm, Shiseido, Tokyo, Japan)
4
Online Oxidation of Melatonin to 6-MOQMA
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The on-line oxidation unit consisted of two 3101 pumps, a 3011 high-pressure valve and a 3019 thermo reactor (NANOSPACE SI-2 series, Shiseido). The flow diagram of the HPLC system used for the evaluation of this on-line oxidation unit is shown in Fig. 2. The eluent of pump 1 was pure water. The reagent added from pump 2 was aqueous Na2CO3, and aqueous H2O2 was added from pump 3. The melatonin solution (10 nM, 50 μL) was injected from the auto sampler, and mixed with the aqueous Na2CO3 and H2O2 solutions. The mixture was introduced into the thermo reactor by switching high-pressure valve 1 and sealed by switching high-pressure valve 1 again, then heated for 30 -90 min. The reaction mixture was then introduced into the concentration column (Asahipak ODP 40-2T, 35 mm × 2.0 mm i.d., 25°C, Shodex, Tokyo, Japan) by switching the high-pressure valves 1 and 2, and washed with pure water (100 μL min -1 , 20 min) provided by pump 1. The concentrated 6-MOQMA was then introduced into the analytical column (CAPCELL PAK C18 ACR, 150 mm × 1.0 mm i.d., 40°C, Shiseido) by switching high-pressure valve 2. The mobile phase of the analytical column was MeCN-TFA-water (12:0.08:88, v/v, 50 μL min -1 ), and the florescence detection of both 6-MOQMA and melatonin was carried out at 370 nm with excitation at 278 nm.
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