Anapoe x 100

Manufactured by Anatrace

Anapoe-X-100 is a non-ionic detergent that can be used for solubilizing and stabilizing membrane proteins. It is a polyethylene glycol ether that has a hydrophobic alkyl chain and a hydrophilic polyethylene glycol head group.

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Lab products found in correlation

Ni sepharose 6 fast flow, by Cytiva (2 mentions) 1 palmitoyl 2 oleoyl glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (2 mentions) Protocatechuate 3 4 dioxygenase, by Merck Group (1 mentions) Adenosine triphosphate (atp), by Roche (1 mentions) Tris 2 carboxyethyl phosphine, by Thermo Fisher Scientific (1 mentions) Glutathione sepharose 4 fast flow, by Cytiva (1 mentions) Mgoac, by Merck Group (1 mentions) Mpeg sva 5000, by Laysan Bio (1 mentions) Biotin peg sva 5000, by Laysan Bio (1 mentions) Gtpγs, by Roche (1 mentions) Benzoic acid, by Merck Group (1 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine n, by Avanti Polar Lipids (1 mentions) N dodecyl β d maltoside, by Avanti Polar Lipids (1 mentions) Edta free protease inhibitor cocktail, by Roche (1 mentions) Streptavidin, by Sangon (1 mentions) Optipreptm density gradient medium, by Merck Group (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) Glycerol, by Merck Group (1 mentions)

3 protocols using anapoe x 100

Lipid Reconstitution and Characterization

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Egg PC, brain PS, brain PI(4)P, C24:1 Galactosyl(ß) Ceramide, Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Tx-DHPE) were from Avanti Polar Lipids. Anapoe-X-100 (Triton X100), DDM was purchased from Anatrace. Peptidol:HS-PEG capped gold nanoparticles of 6 nm were prepared by Dr. L. Duchenes^{49 (link)}; 50 mesh BioBeads were purchased from Bio-Rad and prepared according to ref. ^{50 (link)}. Lacey carbon electron microscopy grids were purchased from Ted Pella (USA).

de la Mora E., Dezi M., Di Cicco A., Bigay J., Gautier R., Manzi J., Polidori J., Castaño-Díez D., Mesmin B., Antonny B, & Lévy D. (2021). Nanoscale architecture of a VAP-A-OSBP tethering complex at membrane contact sites. Nature Communications, 12, 3459.

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Purification and Labeling of Proteins

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Reagents were obtained from the following sources: Ni Sepharose 6 Fast Flow (Cytiva, 17531802), Glutathione Sepharose 4 Fast Flow (Cytiva, 17513201), tris(2-carboxyethyl)phosphine (Thermo, 75259), EDTA-free protease inhibitor cocktail (Roche, 11873580001), PreScission Protease (Absin, abs01243), Anapoe-X-100 (Anatrace, 9002-93-1), ATP (Roche, 11140965001), MgOAc (Sigma-Aldrich, M0631), L-glutamic acid potassium (Sigma-Aldrich, G1501), biotinylated anti-His antibody (Bioss Antibodies, bs-0287R-bio), streptavidin (Sangon Biotech, A610492), LD555-MAL (Lumidyne, 04), LD655-MAL (Lumidyne, 10), GDP (Sigma-Aldrich, G7127), GTPγS (Roche, 10220647001), GMppNHp (Sigma-Aldrich, G0635), mPEG-SVA-5000 (Laysan Bio, 170-106), Biotin-PEG-SVA-5000 (Laysan Bio, 170-124), benzoic acid (Sigma-Aldrich, 242381), protocatechuate 3,4-dioxygenase (Sigma-Aldrich, P8279), and n-dodecyl-β-D-maltoside (Avanti Polar Lipids, 850520). All lipids were obtained from Avanti Polar Lipids: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC, 850457); 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS, 840034); 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE, 810144); and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhodamine-PE, 810150).

Shi L., Yang C., Zhang M., Li K., Wang K., Jiao L., Liu R., Wang Y., Li M., Wang Y., Ma L., Hu S, & Bian X. (2024). Dissecting the mechanism of atlastin-mediated homotypic membrane fusion at the single-molecule level. Nature Communications, 15, 2488.

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Lipid Membrane Reconstitution Protocol

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Reagents were obtained from the following sources: Ni Sepharose 6 Fast Flow (Cytiva, 17531802), tris(2-carboxyethyl)phosphine (TCEP, 75259) (Thermo), Optiprep^TM Density Gradient Medium (60%, w/v) (Sigma-Aldrich, D1556), glycerol (Sigma-Aldrich, G9012), Anapoe-X-100 (Anatrace, 9002-93-1). All DNA oligonucleotides were purchased from Sangon Biotech. All lipids were obtained from Avanti Polar Lipids: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC, 850457); 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE, 850757); 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS, 840034); L-α-phosphatidylinositol- 4,5-bisphosphate [PI(4,5)P₂, 840046]; 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE, 810144); 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhodamine-PE, 810150); 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] [DGS-NTA(Ni), 790404].

Wang Y., Li Z., Wang X., Zhao Z., Jiao L., Liu R., Wang K., Ma R., Yang Y., Chen G., Wang Y, & Bian X. (2023). Insights into membrane association of the SMP domain of extended synaptotagmin. Nature Communications, 14, 1504.

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