Primedirect probe rt qpcr mix
PrimeDirect Probe RT-qPCR Mix is a one-step reverse transcription and real-time quantitative PCR reaction mix. It is designed for the detection and quantification of RNA targets.
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7 protocols using primedirect probe rt qpcr mix
Characterizing Cellular Response to Infection
SARS-CoV-2 RNA Detection by RT-qPCR
SARS-CoV-2 Viral Load Quantification
SARS-CoV-2 RNA Detection from Nasal Swabs
Quantifying SARS-CoV-2 viral load inhibition
Examining BVDV in Mouse Tissues
The fresh feces (500 mg) was obtained from the mouse rectum and soaked in 200 μL pre-cooled diethylpyrocarbonate (DEPC) water for 15 min to dissolve the virus contained in the feces. The DEPC water was centrifuged at 4°C at 10,000 × g for 5 min and the supernatant was stored for subsequent use.
The tissue samples (100 mg) was ground into powder with liquid nitrogen, and 1 mL pre-cooled saline was added. After repeated freeze-thawing, the sample was centrifuged at 10,000 × g at 4°C for 5 min, and the supernatant was obtained.
Total RNA was extracted from the fecal and tissues samples with an RNA extraction kit (Takara Bio, Shiga, Japan). Complementary DNA (cDNA) was synthesized by reverse transcription and the BVDV content was analyzed by qRT-PCR using a Prime Direct™ Probe RT-qPCR Mix (Takara Bio). The relative BVDV content was calculated in relation to that of the normal control.
Quantification of SARS-CoV-2 RNA Levels
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