Cntnap2 null mice were obtained from E. Peles and backcrossed to the C57BL/6J background for over 12 generations. For targeted electrophysiological recordings of parvalbumin-positive interneurons, Cntnap2 heterozygous mice were backcrossed to PV-Cre (Jackson labs number 008069) × Ai9 (Jackson labs number 007909) mice. For spine density analysis, Cntnap2 heterozygous mice were backcrossed with Thy1GFP (Jackson labs × 007788) mice. Experimental mice were obtained from heterozygous crossings and born with the expected Mendelian frequencies; both genders were used. The date of birth was designated at P0 and the three obtained genotypes (wild-type, heterozygous, homozygous knock-out) were housed together with three to four mice per same-sex cage. Mice were kept in a 12-hour light/12-hour dark cycle and had ad libitum access to food and water. All procedures involving animals were performed in accordance with the University of California, Los Angeles (UCLA) animal research committee, and the National Institutes of Health Guide for the Use and Care of Laboratory Animals.
Thy1 gfp
Manufactured by Jackson ImmunoResearch
Thy1-GFP is a fluorescently labeled antibody that binds to the Thy1 (CD90) antigen. Thy1 is a cell surface glycoprotein expressed on various cell types, including T cells, neurons, and mesenchymal stem cells. The Thy1-GFP antibody is conjugated with the green fluorescent protein (GFP) label, allowing for the visualization and identification of Thy1-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Pv cre, by Jackson ImmunoResearch (1 mentions)
Rosa26 eyfp, by Jackson ImmunoResearch (1 mentions)
Six3cre, by Jackson ImmunoResearch (1 mentions)
Cx3cr1creer, by Jackson ImmunoResearch (1 mentions)
Syn1 cre, by Jackson ImmunoResearch (1 mentions)
Rosa26ai9, by Jackson ImmunoResearch (1 mentions)
Cd68 egfp, by Jackson ImmunoResearch (1 mentions)
Gfap cre, by Jackson ImmunoResearch (1 mentions)
3 protocols using thy1 gfp
1
Characterizing Cntnap2 Null Mice Across Neuron Types
2
Generation and Characterization of Ythdf2 Mice
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Ythdf2fl/fl mice were reported previously (Yu et al., 2021b (link)). Six3-cre (Furuta et al., 2000 (link)), Thy1-GFP (Feng et al., 2000 (link)), and Rosa26-eYFP (Srinivas et al., 2001 (link)) mice were from Jackson Laboratory. For timed pregnancy, embryos were identified as E0.5 when a copulatory plug was observed. Genotyping primers are as following: the first Ythdf2-loxP site, 5’-GCTTGTAGTTATGTTGTGTACCAC -3’ and 5’-GCAGCTCTGACTATTCTAAAACCTCC -3’; the second Ythdf2-loxP site, 5’-CTCATAACATCCATAGCCACAGG -3’, and 5’-CCAAGAGATAGCTTTCCTAATG -3’.
Six3-cre site, 5’-CCTTCCTCCCTCTCTATGTG -3’ and 5’-GAACGAACCTGGTCGAAATC -3’.
Rosa26-eYFP wild type site, 5’-CTGGCTTCTGAGGACCG -3’ and 5’-CAGGACAACGCCCACACA -3’; the mutant site, 5’-AGGGCGAGGAGCTGTTCA -3’ and 5’-TGAAGTCGATGCCCTTCAG -3’. All experiments using mice were carried out following the animal protocols approved by the Laboratory Animal Welfare and Ethics Committee of Southern University of Science and Technology.
Six3-cre site, 5’-
Rosa26-eYFP wild type site, 5’-
3
Transgenic Mouse Strains for Neuroscience
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Syn1-Cre (Stock No: 003966), GFAP-Cre (Stock No: 024098), Thy1-GFP (Stock No: 007788), Rosa26Ai9/+ (Stock No: 007909), Fmr1 knockout (Stock No: 003025), CD68-EGFP (Stock No: 026827) and Cx3cr1CreER/+ (Stock No: 021160) mouse strains were obtained from the Jackson Laboratory. The Pten+/− strain was from the National Cancer Institute. All mice were maintained at 22 °C on a 12-h/12-h light/dark cycle with ad libitum access to water and food. Animal procedures were approved by the Scripps Florida Institutional Animal Care and Use Committee.
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