1D and 2D NMR spectra were recorded on a Bruker Avance III 700 spectrometer with a 5 mm TXI cryoprobe (1H NMR: 700 MHz, 13C NMR: 175 MHz) and a Bruker Avance III 500 (1H NMR: 500 MHz, 13C NMR: 125 MHz) spectrometer. Optical rotations were recorded on an Anton Paar MCP 150 polarimeter (sodium D line, Nickel alloy sample cell 100 mm × 3 mm, 0.7 mL). All HPLC–MS analyses were performed on an Agilent 1260 Infinity Systems instrument with a diode array detector and a Waters C18 Acquity UPLC BEH column (2.1 mm × 50 mm, 1.7 μm) using the gradient described by Helaly and co-workers [46 (link)], combined with ion trap MS (Amazon Speed, Bruker) and HRESIMS–TOF (Maxis, Bruker). Chemicals and solvents were obtained from AppliChem GmbH, Avantor Performance Materials, Carl Roth GmbH & Co. KG, and Merck KGaA in analytical and HPLC grades.
Mcp 150
Manufactured by Anton Paar
Sourced in Austria
The MCP 150 is a compact, high-precision digital refractometer designed for measuring the refractive index and concentration of liquids. It provides accurate and reliable results for a wide range of applications, including food and beverage, chemical, and pharmaceutical industries.
Automatically generated - may contain errors
Lab products found in correlation
Avance 3 700, by Bruker (1 mentions)
Acquity uplc beh c18 column, by Waters Corporation (1 mentions)
Amazon speed, by Bruker (1 mentions)
Avance 3 500, by Bruker (1 mentions)
Euroea3000 elemental analyzer, by Eurovector (1 mentions)
Ft ir 620 spectrometer, by Jasco (1 mentions)
Jnm ecz600r, by JEOL (1 mentions)
3 protocols using mcp 150
1
NMR, Polarimetry, and HPLC-MS Analysis
2
Characterization of Xylan Extracts
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The 0.5% (w/v) extracted xylan
from Acacia sawdust and commercial xylans, namely, beechwood xylan
and xylan from Carbosynth, were dissolved separately in 2 mL of 50
mM NaOH solution by heating at 60 °C. The residual undissolved
polysaccharide was removed by centrifuging the sample at 13000g for 15 min, and thereafter, the clear sample was transferred
into a polariscope tube. The specific optical rotation of the extracted
xylan and commercially available xylans was recorded by a digital
polarimeter (MCP150, Anton Paar, GmbH, Austria) at 25 °C maintained
by the Peltier-based temperature controller. The elemental composition
analysis of extracted xylan from Acacia sawdust was performed to estimate
the purity by determining the carbon, hydrogen, nitrogen, and sulfur
(CHNS) content by a CHNS analyzer (EuroEA3000 elemental analyzer,
EuroVector, Italy).
from Acacia sawdust and commercial xylans, namely, beechwood xylan
and xylan from Carbosynth, were dissolved separately in 2 mL of 50
mM NaOH solution by heating at 60 °C. The residual undissolved
polysaccharide was removed by centrifuging the sample at 13000g for 15 min, and thereafter, the clear sample was transferred
into a polariscope tube. The specific optical rotation of the extracted
xylan and commercially available xylans was recorded by a digital
polarimeter (MCP150, Anton Paar, GmbH, Austria) at 25 °C maintained
by the Peltier-based temperature controller. The elemental composition
analysis of extracted xylan from Acacia sawdust was performed to estimate
the purity by determining the carbon, hydrogen, nitrogen, and sulfur
(CHNS) content by a CHNS analyzer (EuroEA3000 elemental analyzer,
EuroVector, Italy).
3
Characterization of Organic Compounds
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General Experimental Procedures Optical rotations were measured with an Anton Paar MCP 150 digital polarimeter. IR spectra were recorded with a JASCO FT-IR/620 spectrometer. 1 (link) H-and 13 C-NMR spectra were recorded on a JEOL JNM-ECZ 600R (600 MHz for 1 H, 150 MHz for 13 C). The 1 Hand 13 C-NMR chemical shifts (δ) in ppm were referenced to the solvent residual peaks for chloroform-d 1 at δ H 7.26, and δ C 77.0. The coupling constant (J) values were measured in hertz. HR-ESI-MS spectra were obtained using a Micromass LCT spectrometer with a time-of-flight (TOF) analyzer. Precoated silica gel plates with a fluorescent indicator (Merck 60 F254) were used for analytical TLC. All reagents and solvents were of commercial quality and were used as received.
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