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Coda monitor

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The CODA Monitor is a non-invasive blood pressure monitoring device. It measures blood pressure and heart rate using an inflatable cuff placed around the animal's tail or limb. The device provides real-time data on systolic, diastolic, and mean arterial pressure, as well as heart rate.

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Nestin cre, by Jackson ImmunoResearch (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Mousestat jr, by Kent Scientific (1 mentions)

41 protocols using coda monitor

1

Noninvasive Blood Pressure Measurement in Mice

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At the age of seven weeks, systolic and diastolic BPs were noninvasively measured from mouse tail using CODA Monitor (Kent Scientific, Torrington, CT, USA).
Suh S.H., Choi H.S., Kim C.S., Kim I.J., Ma S.K., Scholey J.W., Kim S.W, & Bae E.H. (2019). Olmesartan Attenuates Kidney Fibrosis in a Murine Model of Alport Syndrome by Suppressing Tubular Expression of TGFβ. International Journal of Molecular Sciences, 20(15), 3843.
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2

Noninvasive Blood Pressure Measurement in Mice

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Systolic and diastolic blood pressure was obtained in awake mice using a tail cuff noninvasive blood pressure system (CODA Monitor; Kent Scientific Corporation). A minimum of three measurements was obtained from each mouse.
Gogiraju R., Xu X., Bochenek M.L., Steinbrecher J.H., Lehnart S.E., Wenzel P., Kessel M., Zeisberg E.M., Dobbelstein M, & Schäfer K. (2015). Endothelial p53 Deletion Improves Angiogenesis and Prevents Cardiac Fibrosis and Heart Failure Induced by Pressure Overload in Mice. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 4(2), e001770.
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3

Noninvasive Blood Pressure Measurement in Rats

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Systolic blood pressure, diastolic blood pressure, and mean blood pressure were measured noninvasively in conscious animals by a computerized system (CODA Monitor, Kent Scientific) with a volume-pressure recording sensor and an occlusion tail-cuff. Rats were placed in individual holders. The occlusion cuff and the volume-pressure recording cuff were placed close to the base of the tail. After an adaptation period of 30 min on a 37 °C warm pad, 10 preliminary measurements were performed before actual measurement. Rats were trained for restraint for 3 consecutive days prior to the actual measurement. Results are presented as the mean of at least 15 recordings on each occasion taken. The measurements were performed at the same time of a day from 2 to 4 pm by the same investigator as done in our previous studies [19 (link)].
Man A.W., Chen M., Zhou Y., Wu Z., Reifenberg G., Daiber A., Münzel T., Xia N, & Li H. (2020). Fetal programming effects of pentaerythritol tetranitrate in a rat model of superimposed preeclampsia. Journal of Molecular Medicine (Berlin, Germany), 98(9), 1287-1299.
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4

Conscious Mouse Blood Pressure Measurement

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Blood pressure measurements were conducted in conscious restrained mice using a computerized tail-cuff system (CODA® Monitor, Kent Scientific, Torrington, CT, USA). To familiarize the mice with the procedure, they underwent a two-day training period. During measurements, mice were placed in restraint tubes to minimize movement and positioned on a warming platform (maintained at 32–35 °C) to ensure comfort. Before starting the actual experiment, the tails of the mice were gently cuffed, and an acclimatization period of 5 min was allowed to let the mice adjust to the setup. Each measurement session comprised 20 cycles, with the initial 5 cycles designated for acclimatization and excluded from the analysis. The average of the subsequent 15 cycles was recorded as the blood pressure reading for each mouse. Following the experiment, mice were euthanized by CO2 exposure. Blood samples were collected from the heart, and the serum total cholesterol levels were determined using the scil Reflovet® Plus analyzer (scil animal care company GmbH, Viernheim, Germany).
Buonfiglio F., Xia N., Yüksel C., Manicam C., Jiang S., Zadeh J.K., Musayeva A., Elksne E., Pfeiffer N., Patzak A., Li H, & Gericke A. (2023). Studies on the Effects of Hypercholesterolemia on Mouse Ophthalmic Artery Reactivity. Diseases, 11(4), 124.
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5

Comprehensive Physiological Monitoring

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Heart rate and systolic and diastolic arterial blood pressure were monitored using a CODA Monitor (Kent Scientific). For respiration rate, the number of breaths was averaged from three individual 60-s trials. Blood gases and pH were determined from a small sample (∼90 µl) of arterial blood, collected from the cannulated right femoral artery, using the i-STAT CG8+ moderately complex panel (03P88-25; Abbott). For analysis of liver and kidney function, ∼200 µl EDTA-plasma was collected and sent to IDEXX BioResearch for screening (test codes 60405 and 60406, respectively).
Lazic D., Sagare A.P., Nikolakopoulou A.M., Griffin J.H., Vassar R, & Zlokovic B.V. (2019). 3K3A-activated protein C blocks amyloidogenic BACE1 pathway and improves functional outcome in mice. The Journal of Experimental Medicine, 216(2), 279-293.
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6

Systemic Vascular Effects of C60 Exposure

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We assessed maternal blood pressure and resting heart rate in the C60/PVP or PVP exposed groups and in the naïve group to assess any systemic vascular effect resulting from the changes in vascular tissue contractility following in vivo C60 exposure. A CODA Monitor - non-invasive blood pressure system (Kent Scientific, CT, USA) with the tail-cuff was used to follow systemic blood pressure and heart rate changes while the rats were held in rodent restraining bags (AIMS Inc. USA), on a 37 °C heat pad without anesthesia. Rats were acclimated to the tail-cuff three days before blood pressure measurements to minimize changes in blood pressure and heart rate associated with the stress of handling. These measurements were carried out at GD 10 (i.e. mid-second trimester), before exposure and 24 hours post-exposure (i.e. immediately before sacrifice: GD 17–19, mid-third trimester). The mean of three readings from each animal at each time point was used for comparison.
Vidanapathirana A.K., Thompson L.C., Mann E.E., Odom J.T., Holland N.A., Sumner S.J., Han L., Lewin A.H., Fennell T.R., Brown J.M, & Wingard C.J. (2014). PVP formulated Fullerene (C60) increases Rho-kinase dependent Vascular Tissue Contractility in Pregnant Sprague Dawley Rats. Reproductive toxicology (Elmsford, N.Y.), 49, 86-100.
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7

Noninvasive Tail-Cuff Blood Pressure Measurement in Mice

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Blood pressure parameters were measured noninvasively in conscious mice by a computerized system (CODA Monitor, Kent Scientific, Torrington, CT, USA) as described in our previous studies.26–29 (link) Briefly, the occlusion cuff and the volume-pressure recording cuff were placed close to the base of the tail. After an adaptation period of 30 min on a 37°C warm pad, 10 preliminary measurements were performed before the actual measurement. Mice were acclimated for three consecutive days before the actual measurement. Results are presented as the mean of at least 15 recordings on each occasion. The measurements were performed at the same time of a day from 2 pm to 4 pm by the same investigator.
Man A.W., Zhou Y., Reifenberg G., Camp A., Münzel T., Daiber A., Xia N, & Li H. (2023). Deletion of adipocyte NOS3 potentiates high-fat diet-induced hypertension and vascular remodelling via chemerin. Cardiovascular Research, 119(17), 2755-2769.
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8

Measuring Gestational Hypertension in Mice

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Blood pressure was measured with a tail-cuff using a CODA™ monitor (Kent Scientific, Torrington, CT, USA), 5 -10 times at 30 s intervals, 15 min after the behavior and heart rate of the pregnant mice were stabilized in the morning of 5.5, 9.5, 13.5, 15.5, and 17.5 dpc.
Blood pressure values are reported as the means of at least three measurements varying by 5% obtained in one session.
Kojima T., Yamada T., Akaishi R., Furuta I., Saitoh T., Nakabayashi K., Nakayama K.I., Nakayama K., Akira S, & Minakami H. (2015). Role of the Atg9a gene in intrauterine growth and survival of fetal mice. Reproductive biology, 15(3).
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9

Non-invasive Blood Pressure Monitoring

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BP was measured by a tail-cuff system (CODA Monitor; Kent Scientific Corporation, Torrington, CT, USA) essentially as described previously8 (link). Briefly, at the same time each morning, BP was recorded for 5 acclimation cycles followed by 30–35 measurement cycles. The three highest and lowest readings were removed and an average of the remaining recordings used to calculate the daily BP. An average of 3 days was used to calculate the BP at day 0 before the diet was changed, and an average of BP at post-diet days 13, 14, and 16 used to calculate the final BP. Heating pads were used to keep the mice warm throughout the experiment to ensure sufficient blood flow to the tail.
Manning J.A., Shah S.S., Nikolic A., Henshall T.L., Khew-Goodall Y, & Kumar S. (2021). The ubiquitin ligase NEDD4-2/NEDD4L regulates both sodium homeostasis and fibrotic signaling to prevent end-stage renal disease. Cell Death & Disease, 12(4), 398.
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10

Non-Invasive Murine Blood Pressure Monitoring

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Systolic, diastolic and mean blood pressure were measured using the non-invasive tail cuff method (CODA Monitor, Kent Scientific Corp). At the start of a blood pressure measurement session, the appropriate mouse holder was selected based on the mouse’s weight. The holder was placed over a heating pad with a set temperature that is regulated at 38 °C. The tail-cuff and Volume Pressure Recording (VPR) sensor were placed on the heating pad and covered with a blanket to allow these components to reach the set temperature. After 2–3 minutes, the awake mouse was gently introduced into the holder. A light blanket was draped over the tail and the mouse was left alone for 3–5 minutes to allow habituation. The blood pressure measurements take place via 10–20 tail cuff inflation-deflation sweeps that in total take from 5–10 minutes in duration. Multiple days of measurements may be required to gain confidence in the accuracy of the measurements. After the measurements, the mouse was carefully removed from the holder and immediately placed in its cage. Measurements of all sweeps are then averaged per mouse.
Chow B.W., Nuñez V., Kaplan L., Granger A.J., Bistrong K., Zucker H.L., Kumar P., Sabatini B.L, & Gu C. (2020). Caveolae in the CNS arterioles mediate neurovascular coupling. Nature, 579(7797), 106-110.
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