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Fmoc arg pbf oh

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Fmoc-Arg(Pbf)-OH is a protected amino acid building block commonly used in solid-phase peptide synthesis. It consists of the Fmoc-protected arginine amino acid with a Pbf (2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl) side-chain protecting group. This product is a key intermediate in the synthesis of peptides and proteins.

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Lab products found in correlation

Fmoc ala oh, by AAPPTec (5 mentions) Fmoc lys boc oh, by AAPPTec (4 mentions) Fmoc glu otbu oh, by AAPPTec (4 mentions) Fmoc ile oh, by AAPPTec (4 mentions) Fmoc thr tbu oh, by AAPPTec (4 mentions) Fmoc cys trt oh, by AAPPTec (4 mentions) Fmoc asp otbu oh, by AAPPTec (3 mentions) Fmoc ser tbu oh, by AAPPTec (3 mentions) Fmoc asn trt oh, by AAPPTec (3 mentions) Fmoc pro oh, by AAPPTec (3 mentions) Fmoc his trt oh, by AAPPTec (3 mentions) Fmoc trp boc oh, by AAPPTec (3 mentions) Fmoc gln trt oh, by AAPPTec (3 mentions) Fmoc leu oh, by AAPPTec (3 mentions) Rink amide resin, by AAPPTec (3 mentions) Acetonitrile, by Merck Group (3 mentions) Isopropanol, by Merck Group (3 mentions) Trifluoroacetic acid, by Merck Group (3 mentions) Dichloromethane, by Merck Group (3 mentions) Phenol, by Merck Group (2 mentions)

6 protocols using fmoc arg pbf oh

1

Automated Peptide Synthesis and Characterization

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Fmoc-Arg(Pbf)-OH, Fmoc-Ala-OH, and Fmoc-Asp(OtBu)-OH were purchased from the Aapptec (Louisville, USA) and used as received. N,N-dimethylformamide (DMF), N,N-diisopropylethylamine (DIPEA), N-methyl-2-pyrrolidone (NMP), trifluoroacetic acid (TFA) and triisopropylsilane were purchased from VWR (Radnor, USA). N,N,N′,N′-tetramethyl-O-(1H-benzotriazol-1-yl)uronium hexafluorophosphate (HBTU), 1-hydroxybenzotriazole hydrate (HoBT), 4-metylpiperidin and Thioflavin T were purchased from Sigma-Aldrich. HPLC grade water (resistivity 18 MΩ cm) and DPBS (pH 7.4) were purchased from Thermo Fisher scientific (Waltham, USA).
Lammi C., Bollati C., Gelain F., Arnoldi A, & Pugliese R. (2019). Enhancement of the Stability and Anti-DPPIV Activity of Hempseed Hydrolysates Through Self-Assembling Peptide-Based Hydrogels. Frontiers in Chemistry, 6, 670.
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2

Solid-Phase Peptide Synthesis Protocol

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The gold electrodes were purchase from DropSens (Asturias, Spain) and BASi (West Lafayette, IN, USA), Rink amide resin (0.46 meq/g), Fmoc-Arg(Pbf)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Thr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Pro-OH, Fmoc-Cys(Trt)-OH, Fmoc-His(Trt)-OH, Fmoc-Lys(ivDde)-OH, Fmoc-Lys(Fmoc)-OH, 6-Fmoc-aminohexanoic acid (Fmoc-Ahx-OH), Fmoc-Tyr(tBu)-OH, 1-hydroxybenzotriazole (HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), acetic anhydride, piperidine, pyridine, ninhydrin, phenol, ferrocene, ferrocenecarboxylic acid, Freund’s incomplete and complete adjuvant, and KCN were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichlorometane (DCM), acetonitrile (AcN), isopropyl alcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, MI, USA). Analytical grade KOH, KCl, Ru(NH3)6Cl3, K3Fe(CN)6, and H2SO4 were acquired from J.T. Baker. The pH was adjusted with either KOH or HCl solutions obtained from J. T. Baker. All aqueous solutions were prepared with deionized water (ρ ≥ 18 MΩ cm−1). All reagents were used without further purification.
Lara Carrillo J.A., Fierro Medina R., Manríquez Rocha J., Bustos Bustos E., Insuasty Cepeda D.S., García Castañeda J.E, & Rivera Monroy Z.J. (2017). Design, Synthesis, and Use of Peptides Derived from Human Papillomavirus L1 Protein for the Modification of Gold Electrode Surfaces by Self-Assembled Monolayers. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(11), 1970.
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3

Solid-Phase Peptide Synthesis Protocol

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Mueller-Hinton, Agar SPC, E. coli ATCC 25922, and E. faecalis ATCC 29212 were obtained from ATCC, USA. Rink amide resin, Fmoc-Arg(Pbf)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Gln(Trt)-OH, Fmoc-β-Ala-OH, Fmoc-Phe-OH, Fmoc-Met-OH, Fmoc-Leu-OH, Fmoc-Gly-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Glu(OtBu)OH, Fmoc-Thr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Pro-OH, Fmoc-Cys(Trt)-OH, Fmoc-Lys(Fmoc)-OH, 6-(Fmoc-amino)hexanoic acid (Fmoc-Ahx-OH), 1-hydroxybenzotriazole (HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). N,N-Diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-Ethanedithiol (EDT), 4-methyl-piperidine, pyridine, ninhydrin, phenol, and KCN were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichloromethane (DCM), acetonitrile (ACN), isopropyl alcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, Michigan, USA). All reagents were used without further purification.
León-Calvijo M.A., Leal-Castro A.L., Almanzar-Reina G.A., Rosas-Pérez J.E., García-Castañeda J.E, & Rivera-Monroy Z.J. (2015). Antibacterial Activity of Synthetic Peptides Derived from Lactoferricin against Escherichia coli ATCC 25922 and Enterococcus faecalis ATCC 29212. BioMed Research International, 2015, 453826.
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4

Solid-Phase Peptide Synthesis of Leu-Arg-Trp-Gln

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Reagents Rink amide resin, Fmoc-Leu-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Gln(Trt))-OH, Dicyclohexylcarbodiimide (DCC), and 1-Hydroxy-6-chlorobenzotriazole (6-Cl-HOBt) were purchased from AAPPTec (Louisville, KY, USA). Reagents acetonitrile (ACN), trifluoroacetic acid (TFA), dichloromethane (DCM), diisopropylethylamine (DIPEA), N,N-dimethylformamide (DMF), ethanedithiol (EDT), isopropanol (IPA), methanol, and triisopropylsilane (TIS) were acquired from Merck (Darmstadt, Germany). SPE Supelclean™ LC-18 columns were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trypticase Soy Agar (TSA), Mueller Hinton Agar (MHA), and Plate Count Agar (PCA) media were purchased from Scharlau. Mueller Hinton Broth medium was purchased from Merck. The Escherichia coli ATCC 25922 bacterial strain was purchased from the ATCC. The MCF-7 and HeLa cell lines were obtained from ATCC® (Manassas, VA, USA). All the reagents were used directly, without previous purification.
1H spectra were recorded at 400 MHz on a Bruker Advance 400 instrument. RP-HPLC analyzes were performed on a Chomolith C18 column (Merck, Kenilworth, NJ, USA, 50 mm), using an Agilent 1200 Liquid Chromatograph (Agilent, Omaha, NE, USA). The products were analyzed on a Bruker Impact 2 LC Q-TOF MS equipped with electrospray ionization (ESI) in positive mode and on a Bruker MicroFlex MALDI-TOF mass spectrometer.
Pineda-Castañeda H.M., Maldonado-Villamil M., Parra-Giraldo C.M., Leal-Castro A.L., Fierro-Medina R., Rivera-Monroy Z.J, & García-Castañeda J.E. (2023). Peptide-Resorcinarene Conjugates Obtained via Click Chemistry: Synthesis and Antimicrobial Activity. Antibiotics, 12(4), 773.
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5

Solid-Phase Peptide Synthesis Reagents

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The Rink amide resin and Fmoc-Gly-OH, Fmoc-Ala-OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Cys(Trt)-OH, Fmoc-Met-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-His(Trt)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Gln(Trt)-OH, Fmoc-Pro-OH, Fmoc-Ahx, N,N-Dicyclohexylcarbodiimide, and 1-Hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). The reagents such as, acetonitrile, trifluoroacetic acid, dichloromethane, diisopropylethylamine, N, N-dimethylformamide, ethanedithiol, isopropanol, methanol, and triisopropylsilane were purchased from Merck (Darmstadt, Germany). SupelcleanTM SPE columns were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Silicycle® SiliaPrepTM C18 columns were kindly donated by EcoChem Especialidades Químicas (Waterloo, QC, Canada).
Insuasty Cepeda D.S., Pineda Castañeda H.M., Rodríguez Mayor A.V., García Castañeda J.E., Maldonado Villamil M., Fierro Medina R, & Rivera Monroy Z.J. (2019). Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology. Molecules, 24(7), 1215.
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6

Synthesis of Peptide-Based Compounds

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The resin Rink amide and the reagents Fmoc-Ser(tBu)-OH, Fmoc-Pro-OH, Fmoc-Ile-OH, Fmoc-Asn(Trt)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Lys(Boc)-OH, Fmoc-His(Trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Ala-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Cys(Trt)-OH, Fmoc-6-aminohexanoic acid (Fmoc-Ahx-OH), dicyclohexylcarbodiimide, and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). The reagents acetonitrile, trifluoroacetic acid, dichloromethane, diisopropylethylamine, N,N-dimethylformamide, ethanedithiol, isopropanol, methanol, and triisopropylsilane were purchased from Merck (Darmstadt, Germany). SPE columns Supelclean™, 6-maleimidohexanoic acid (6-MhxA), tetrabutylammonium chloride, glycidyl methacrylate (GMA), ethylene dimethacrylate (EDMA), cyclohexanol, dodecanol, 1,1′-azobis(cyclohexanecarbonitrile), silica gel, Tween 20, and 3,3′,5,5′-tetramethylbenzidine (Liquid Substrate System for ELISA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Potassium diacid phosphate, sodium acid phosphate, sodium chloride, and potassium chloride were purchased from AppliChem Panreac (Barcelona, Spain).
Insuasty-Cepeda D.S., Maldonado M., García-Castañeda J.E, & Rivera-Monroy Z.J. (2021). Obtaining an immunoaffinity monolithic material: poly(GMA-co-EDMA) functionalized with an HPV-derived peptide using a thiol–maleimide reaction. RSC Advances, 11(7), 4247-4255.
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