Easy nlc1000 hplc pump

Manufactured by Thermo Fisher Scientific

The Easy-nLC 1000 is a high-performance liquid chromatography (HPLC) pump designed for nanoscale separations. It delivers precise and consistent flow rates for a variety of applications, including proteomics, metabolomics, and lipidomics. The pump features a compact design and easy-to-use interface, making it a reliable and efficient solution for liquid chromatography workflows.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

NLC1000 (15 citations)

6 protocols using easy nlc1000 hplc pump

VIC Peptide Analysis by Orbitrap Fusion

Check if the same lab product or an alternative is used in the 5 most similar protocols

VIC peptide samples were analyzed on the Orbitrap Fusion Lumos mass spectrometer fronted with an EASY-Spray Source (heated at 45 °C) and coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific). Peptides were subjected to a dual-column setup: an Acclaim PepMap RSLC C18 trap analytical column, 75 μm × 20 mm (precolumn) and an EASY-Spray LC column, 75 μm × 250 mm (Thermo Fisher Scientific). The analytical gradient was run at 300 nl/min from 5 to 21% solvent B (acetonitrile/0.1% formic acid) for 75 min, 21 to 30% solvent B for 15 min, followed by 10 min of 95% solvent B. Solvent A was water/0.1% formic acid. The acetonitrile and water were of MS grade. The Orbitrap analyzer was set to 120 K resolution, and the top N precursor ions in 3 s cycle time within a scan range of 375 to 1500 m/z (60 s dynamic exclusion enabled) were subjected to collision-induced dissociation (collision energy, 30%; isolation window, 1.6 m/z; automatic gain control target, 1.0 e4). The ion trap analyzer was set to a rapid scan rate for peptide sequencing (MS/MS).

Schlotter F., de Freitas R.C., Rogers M.A., Blaser M.C., Wu P.J., Higashi H., Halu A., Iqbal F., Andraski A.B., Rodia C.N., Kuraoka S., Wen J.R., Creager M., Pham T., Hutcheson J.D., Body S.C., Kohan A.B., Sacks F.M., Aikawa M., Singh S.A, & Aikawa E. (2021). ApoC-III is a novel inducer of calcification in human aortic valves. The Journal of Biological Chemistry, 296, 100193.

+ Open protocol

+ Expand

Orbitrap Fusion Lumos Mass Spectrometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All peptide samples were analyzed
on an Orbitrap Fusion Lumos mass spectrometer fronted with an EASY-Spray
Source, coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific).
The peptides were subjected to a dual column setup: an Acclaim PepMap
RSLC C18 trap column, 75 μm × 20 mm (Thermo Fisher Scientific,
Cat# 164261); and an EASY-Spray LC Column, 75 μm × 250
mm (Thermo Fisher Scientific, Cat# ES802). The analytical gradient
was run at 300 nL/min from 5 to 21% Solvent B (acetonitrile/0.1% formic
acid) for 50 min, 21 to 30% Solvent B for 10 min, and 95% Solvent
B for 5 min. Solvent A was water/0.1% formic acid.

Higashi H., Maejima T., Lee L.H., Yamazaki Y., Hottiger M.O., Singh S.A, & Aikawa M. (2019). A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation. Journal of Proteome Research, 18(4), 1607-1622.

+ Open protocol

+ Expand

Proteomic Profiling of ADPr Peptides

Check if the same lab product or an alternative is used in the 5 most similar protocols

ADPr (MARylated) peptides from control, saline, and IFN-γ elicited mouse tissues (spleen and liver) were analyzed using the Orbitrap Fusion Lumos fronted with an EASY-Spray Source, coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific), and the Q Exactive Orbitrap (+Easy-nLC1000) fronted with a Nanospray FLEX ion source (Thermo Fisher Scientific).

Kuraoka S., Higashi H., Yanagihara Y., Sonawane A.R., Mukai S., Mlynarchik A.K., Whelan M.C., Hottiger M.O., Nasir W., Delanghe B., Aikawa M, & Singh S.A. (2021). A Novel Spectral Annotation Strategy Streamlines Reporting of Mono-ADP-ribosylated Peptides Derived from Mouse Liver and Spleen in Response to IFN-γ. Molecular & Cellular Proteomics : MCP, 21(4), 100153.

+ Open protocol

+ Expand

Orbitrap Fusion Lumos Tribrid Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The peptides were analyzed using the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) fronted with an Easy-Spray ion source and coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific). The peptides were separated using a dual column set-up: An Acclaim PepMap RSLC C18 trap column, 75 μm X 20 mm; and an EASY-Spray LC heated (45°C) column, 75 μm × 250 mm (Thermo Fisher Scientific). The gradient flow rate was 300 nl/min from 5 to 21% solvent B (acetonitrile/0.1% formic acid) for 75 min, 21 to 30 % Solvent B for 15 min, followed by 10 min of a ‘jigsaw wash’, alternating between 5 and 95 % Solvent B. Solvent A was 0.1% formic acid. The instrument was set to 120 K resolution, and the top N precursor ions in a 3 second cycle time (within a scan range of 375–1,500 m/z; isolation window, 1.6 m/z; ion trap scan rate, normal) were subjected to collision induced dissociation (collision energy 30%) for peptide sequencing (or MS/MS). Dynamic exclusion was enabled (60 s).

Lupieri A., Nagata Y., Passos L.S., Beker-Greene D., Kirkwood K.A., Wylie-Sears J., Alvandi Z., Higashi H., Hung J.W., Singh S.A., Bischoff J., Levine R.A, & Aikawa E. (2021). Integration of Functional Imaging, Cytometry, and Unbiased Proteomics Reveals New Features of Endothelial-to-Mesenchymal Transition in Ischemic Mitral Valve Regurgitation in Human Patients. Frontiers in Cardiovascular Medicine, 8, 688396.

+ Open protocol

+ Expand

Unbiased Peptide Sampling and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Data-dependent acquisitions (DDAs, unbiased peptide sampling)–the peptides were and analyzed using the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) fronted with an EasySpray ion source, and coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific). The peptides were separated using a dual column set-up: an Acclaim PepMap 100 C18 trap column, 75 μm × 20 mm; and a PepMap RSLC C18 EASY-Spray LC heated (45°C) column, 75 μm × 250 mm (Thermo Fisher Scientific). The gradient flow rate was 300 nl/min from 5 to 21% solvent B (acetonitrile/0.1% formic acid) for 75 min, 21 to 30 % Solvent B for 15 min, followed by 10 min of a “jigsaw wash,” alternating between 5 and 95 % Solvent B. Solvent A was 0.1% formic acid. The instrument was set to 120 K resolution, and the top N precursor ions in a 3 s cycle time (within a scan range of 375–1,500 m/z; isolation window, 1.6 m/z; ion trap scan rate, normal) were subjected to collision induced dissociation (collision energy 30%) for peptide sequencing (or MS/MS). Dynamic exclusion was enabled (60 s).

Atkins S.K., Sonawane A.R., Brouwhuis R., Barrientos J., Ha A., Rogers M., Tanaka T., Okui T., Kuraoka S., Singh S.A., Aikawa M, & Aikawa E. (2022). Induced pluripotent stem cell-derived smooth muscle cells to study cardiovascular calcification. Frontiers in Cardiovascular Medicine, 9, 925777.

+ Open protocol

+ Expand

Evaluating Technical Variation in Tracer Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

The aim of this study was to understand sources of technical variation and limitation on the acquisition and fidelity of low abundant tracer in vivo, not to match or compare the performance of the Q Exactive with the technically more advanced Lumos platform, per se. Thus, differences in each platform’s constitution (including peripheral devices, such as the column type and temperature) are expected to affect the data quality. However, these differences are minor compared with, for instance, the differences in the ion inlet and optics guiding the eluted peptides into the mass spectrometer (https://planetorbitrap.com, ref. 36 (link)). As a consequence, we do not expect that differences in the columns affect the differences in data quality described in this study. Both Q Exactive (quadrupole + Orbitrap) and Orbitrap Fusion Lumos (quadrupole + linear ion trap + Orbitrap) instruments were coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific). The Lumos was fronted with an EASY-Spray ion source and the Q Exactive with a Nanospray FLEX ion source (Thermo Fisher Scientific).

Singh S.A., Andraski A.B., Higashi H., Lee L.H., Ramsaroop A., Sacks F.M, & Aikawa M. (2021). Metabolism of PLTP, CETP, and LCAT on multiple HDL sizes using the Orbitrap Fusion Lumos. JCI Insight, 6(3), e143526.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!