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5 protocols using dextrose

1

Analytical Quantification of Metabolites

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All reagents and chemicals were of analytical grade and applied without further purification. HPLC-grade deionized water was used during all experiments.
α-KG, 5-HMF, NASeLM, NALM, and glucose were supplied by CYL-Pharma (Lassnitzhöhe, Austria). NaCl, KCl, KH2PO4, MgSO4, CaCl2, NaHCO3, and dextrose were obtained from Roth (Karlsruhe, Germany). Acetonitrile, ammonium acetate, 1-butanol, ethanol, HPLC-grade water, hydrogen peroxide, and HCl were obtained from Merck (Darmstadt, Germany). Albumin from human serum, angiotensin 1-7 acetate salt hydrate, butylated hydroxytoluene (BHT), 2,4-dinitrophenylhydrazine (DNPH), ethyl acetate, guanidine-hydrochloride, malondialdehyde tetra-butyl-ammonium salt (MDA), 2-thiobarbituric acid (TBA), trichloroacetic acid (TCA), and tris(hydroxymethyl)aminomethane (Tris) were obtained from Sigma-Aldrich (Vienna, Austria).
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2

Actinomycetes Isolates Culture Protocol

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The Actinomycetes isolates were cultured in yeast extract malt extract agar medium (ISP medium No. 2), prepared by dissolving 10 g malt extract (LobaChemie), 4 g yeast extract (LobaChemie), 4 g dextrose (Carl Roth GmbH), and 20 g agar (Carl Roth GmbH) in 1 L distilled water. Aerial mycelium color was recorded after the strains had reached abundant sporulation. In cases where the aerial mycelium color fell between two colors series, both colors were recorded. The color of the substrate mycelium was determined by observing the reverse (bottom) side of mycelium growth on the ISP 2 medium.
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3

Drosophila Rearing and Maintenance

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In the present study W1118 wild-type Drosophila melanogaster were used for all experiments. Flies were maintained under conventional conditions on 10% Caltech (CT) medium (5.5% dextrose, 3.0% sucrose [both Carl Roth, Karlsruhe, Germany], 6.0% corn meal, 2.5% inactive dry yeast, 1.0% agar, 0.3% nipagin [all Dominique Dutscher SAS, Brumath, France] and 0.3% propionic acid [Carl Roth, Karlsruhe, Germany]) in a constant climate chamber (HPP 1018, Memmert, Schwabach, Germany) with a temperature of 25°C, 60% relative humidity and a 12-h day/night cycle. For all experiments, age-matched flies from synchronized eggs were used.
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4

Culturing Drosophila melanogaster w1118 Flies

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D. melanogaster strain w1118 (Bloomington Drosophila Stock Center #5905, Indiana University, Bloomington, IN, USA) was used for all experiments. Flies were maintained on modified Caltech (CT) medium (6.0% dextrose, 3.0% sucrose (Carl Roth, Karlsruhe, Germany), 6.0% cornmeal (Kisker, Steinfurt, Germany), 3.0% inactive dry yeast (contributing 1.6% protein, 1% complex carbohydrates and 0.15% fat as well as vitamins and minerals according to the product information), 1.0% agar, 1.0% nipagin (Genesee Scientific, San Diego, CA, USA) (dissolved in ethanol (20% w/v) (VWR, Radnor, PA, USA) and 0.3% propionic acid (Carl Roth, Karlsruhe, Germany)) in Drosophila storage bottles (Kisker, Steinfurt, Germany) at 25 °C and 60% relative humidity with a 12/12 h light/dark cycle in a climate chamber (Memmert, Schwabach, Germany) [32 (link)].
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5

HPLC-grade Deionized Water Protocol

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HPLC-grade deionized water was applied during all experiments including Langendorff studies. All chemicals and reagents were of analytical grade and used without further purification.
NaCl, KCl, KH2PO4, MgSO4, CaCl2, NaHCO3, and dextrose were purchased from Roth (Karlsruhe, Germany). α-KG, 5-HMF, NALM, NASeLM, and glucose were supplied by CYL-Pharma (Lassnitzhöhe, Austria). Human albumin solution 20% was provided from Behring (Marburg, Germany). Acetonitrile, NH4CH3COO, 1-butanol, ethanol, HPLC-grade water, and HCl were obtained from Merck (Darmstadt, Germany). 2-Thiobarbituric acid, butylated hydroxytoluene, ethyl acetate, trichloroacetic acid solution, 2,4-dinitrophenylhydrazine (DNPH), malondialdehyde tetrabutylammonium salt, guanidine hydrochloride, and tris(hydroxymethyl) aminomethane were supplied by Sigma-Aldrich (Vienna, Austria).
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