Rag2 mice

Rag1^–/–, Rag2^–/– mice, Foxp3^Cre-YFP, and Thy1.1 mice were purchased from Jackson laboratory. Il33^–/– mouse from Riken Center for Biosystems Research (accession number CDB0631K). Setd2 ^flox/flox(Setd2 ^f/f) mouse was generated previously^{29 (link)}. Mice labeled with Foxp3^Cre-YFP(Foxp3^Cre) are Foxp3^Cre-YFP/Y for males and Foxp3^{Cre-YFP/Cre-YFP} for females. Mice labeled with the genotype Foxp3^Cre-YFP/+ (Foxp3^Cre/+) are females. Mice used for in vivo studies were littermates, age-matched, and were 6–12 weeks old unless otherwise specified. Both male and female mice were used unless otherwise noted. All mice used in this study are on a C57BL/6 background, maintained in specific pathogen-free conditions, fed with a plain commercial diet (Silaikang, Shanghai), and housed in corn-cob-bedding cages in a room with the light-dark cycle (lights on at 6:00 and off at 18:00). The mice were kept at a constant temperature of 22 ± 3 °C and a relative humidity of 35 ± 5%. All animal experiments were performed in compliance with the “Guide for the Care and Use of Laboratory Animals” and approved by the institutional biomedical research ethics committee of the Shanghai Institutes for Nutrition and Health, Chinese Academy of Sciences, under the protocol numbers SINH-2020-QJ-2, SINH-2021-QJ-1, and SINH-2022-QJ-1.

Kras^LSL-G12D/+;p53^flox/flox (KP) mice were used as a conditional mouse model of soft tissue sarcoma and bred in our laboratory in the C57BL/6 background. Braf^V600E;Pten^–/– (BP) mice (B6.Cg-Braf^tm1Mmcm Pten^tm1Hwu Tg(Tyr-cre/ERT2)13Bos/BosJ) were used as a conditional mouse model of melanoma and bred in our laboratory in the FVB/N background. C57BL/6 wild type mice and Rag2^–/– mice were obtained from the Jackson Laboratory. All animal experiments were approved by the MGH Institutional Animal Care and Use Committee (IACUC) and were performed in accordance with MGH IACUC regulations.

C57BL/6 (Stock No: 000664, Jackson Laboratories), Lpar2^−/− mice (41 (link)) (gift from Dr. Jerold Chun, Scripps Research Institute), Lpar5^−/− mice (39 (link)), CD45.1 OT-I mice (gift from Ross Kedl, Ph.D., University of Colorado), Lpar5^−/− OT-I mice, and Rag2^−/− mice (Stock No: 008449, Jackson Laboratories), Enpp2^+/− mice (42 (link)) (gift from Dr. Susan Smyth, University of Kentucky) were bred and housed at the University of Colorado Anschutz Medical Campus Vivarium (Aurora, CO, USA). OT-I mice (43 (link)) harbor CD8 T cells with a transgenic Vβ5Vα2 TCR specific for the SIINFEKL peptide from chicken ovalbumin (residues 257–264) but also recognize SIIGFEKL with lower affinity (44 (link)). Splenocytes from Lpar6^−/− and Nur77^GFP OT-I mice (45 (link)) mice were gifted from Dr. Iain McKillop (Atrium Health, Carolinas Medical Center University) and Dr. Ross Kedl, respectively. All procedures with animals were approved by the University of Colorado Institutional Animal Care and Use Committee.