G1128

Manufactured by Merck Group

The G1128 is a laboratory equipment product manufactured by the Merck Group. It is designed for general use in scientific research and analysis. The core function of the G1128 is to provide a reliable and precise measurement of various parameters. Further details about its intended use or specific applications are not available.

Automatically generated - may contain errors

Lab products found in correlation

H4784, by Merck Group (1 mentions) Cas 30525 89 4, by Santa Cruz Biotechnology (1 mentions) Cryostar nx70, by Thermo Fisher Scientific (1 mentions) S0389, by Merck Group (1 mentions) Axio imager 2, by Zeiss (1 mentions) Prolong gold, by Thermo Fisher Scientific (1 mentions) L9393, by Merck Group (1 mentions) Catalase, by Merck Group (1 mentions) C2506, by Merck Group (1 mentions) Histomount, by Thermo Fisher Scientific (1 mentions)

3 protocols using g1128

Cardiac Tissue Preparation for Immunostaining

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animals were anaesthetized in 0.1% MS222 (Sigma, A5040). Their hearts were excised, washed in 70% PBS with heparin (100 units ml⁻¹; Sigma, H4784) and fixed with 4% paraformaldehyde for 2 h at room temperature (RT) or overnight at 4 °C (Santa Cruz, CAS 30525-89-4) for immunostaining and rinsed three times before being exposed to 10, 20 and 30% sucrose (Sigma, S0389) solutions at 4 °C. The tissues were then equilibrated to O.C.T. compound (Tissue-Tek, 4583) by immersing them in a 1:1 mixture of 30% sucrose and O.C.T., followed by 100% O.C.T. and finally frozen. Sections (10–14 µm) were prepared on a cryostat at −14 °C (Thermo, Cryostar NX70) in the frontal plane and stored at −80 °C.
To obtain fresh frozen tissue sections, hearts were mounted in 7% tragacanth (Sigma, G1128), dipped in pre-cooled 2-metyhlbutane (Sigma, 277258) and frozen in liquid nitrogen.

Eroglu E., Yen C.Y., Tsoi Y.L., Witman N., Elewa A., Joven Araus A., Wang H., Szattler T., Umeano C.H., Sohlmér J., Goedel A., Simon A, & Chien K.R. (2022). Epicardium-derived cells organize through tight junctions to replenish cardiac muscle in salamanders. Nature Cell Biology, 24(5), 645-658.

+ Open protocol

+ Expand

Muscle Fiber Analysis Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

TA samples were mounted in tragacanth (Sigma-Aldrich # G1128) on plastic blocks and frozen in liquid isopentane cooled in liquid nitrogen and stored at -80°C. Samples were cut into 10μm cross-sections using a cryostat at -20°C then mounted on lysine coated slides (Superfrost), as described in.⁶⁹ (link)^,⁷³ (link) Cross-sections were brought to room temperature, rehydrated with PBS (pH 7.2), then blocked with goat serum (10% in PBS). They were then incubated with primary polyclonal anti-laminin rabbit IgG antibody (Sigma-Aldrich # L9393, 1:500) for 1 h at room temperature. Sections were then washed three times in PBS before being incubated for 1 h at room temperature with an Alexa Fluor® 594 goat anti-rabbit IgG antibody (A-11037, 1:500). Sections were then washed three times in PBS and slides were cover slipped using Prolong™ Gold (P36930, Invitrogen) as a mounting medium. Slides were imaged with a Zeiss fluorescence microscope (Zeiss Axio Imager 2). Median distribution of minimum Feret diameters of at least 300 fibers per muscle sample were analyzed using ImageJ (NIH, Bethesda, MD).⁷⁴ (link) The degree of myofiber atrophy was calculated as percent difference in mean minimum Feret diameters, relative to muscles of sham Atg7^f/f mice. The means, fibers distributions, and average number of fibers analyzed per muscle per mouse are shown in Figure S6.

Leduc-Gaudet J.P., Miguez K., Cefis M., Faitg J., Moamer A., Chaffer T.J., Reynaud O., Broering F.E., Shams A., Mayaki D., Huck L., Sandri M., Gouspillou G, & Hussain S.N. (2023). Autophagy ablation in skeletal muscles worsens sepsis-induced muscle wasting, impairs whole-body metabolism, and decreases survival. iScience, 26(8), 107475.

+ Open protocol

+ Expand

Quantitative Muscle Fiber Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Freshly isolated Gastrocnemius muscles were placed in 8% tragacanth (G1128, Sigma) and frozen in liquid nitrogen-cooled isopentane before cutting 10 μm cryo-cross-sections.
Succinate dehydrogenase (SDH) staining: sections were exposed to 50 mM sodium succinate (S2378, Sigma) in 0.1 M phosphate buffer in the presence of 0.5 mg/ml nitroblue tetrazolium (N5514, Sigma) for 30 min at 37 °C. Then sections were washed with ddH₂O, dehydrated with ethanol and mounted with histomount (008030, Thermo Scientific).
Cytochrome oxidase (COX) staining: slides were exposed to 0.5 mg/ml 3,3′-Diaminobenzidine tetrahydrochloride hydrate (DAB, Sigma D5637), 0.2 mg/ml cytochrome c (C2506, Sigma) and 0.125 mg/ml catalase (C40, Sigma) in PBS for 1 h at 37 °C. Then slides were washed with ddH₂O, dehydrated with ethanol and mounted with histomount (008030, Thermo Scientific).
Fiber typing was carried out as described in the Supplemental Experimental Procedures. Whole sections were pictured using a slide scanner (Axio Scan.Z1, Zeiss). For minFeret measurements and fiber typing counting, square pictures from total sections were cropped out (mean of two pictures in the oxidative part of the muscle, one in the glycolytic part of the muscle). For minFeret determination, a Fiji script was used as described in the Supplemental Experimental Procedures.

Schmid S., Heim-Kupr B., Pérez-Schindler J., Mansingh S., Beer M., Mittal N., Ehrenfeuchter N, & Handschin C. (2022). PGC-1β modulates catabolism and fiber atrophy in the fasting-response of specific skeletal muscle beds. Molecular Metabolism, 66, 101643.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!