Plasmid encoding human IL-12 (pUMVC3-hIL12) was transformed into Escherichia coli bacterial strain DH5α, incubated in Luria-Bertani (LB) media and then extracted and purified from the culture pellets using the Qiagen Endofree Mega Plasmid Kit according to the manufacturer’s instructions. The purity and the concentration of the plasmid were assessed using a UV–visible spectrometer (Shimadzu, Japan) at 260 and 280 nm.
Uv visible spectrometer
Manufactured by Shimadzu
Sourced in Japan
The UV–visible spectrometer is an analytical instrument used to measure the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum. It is designed to quantify the concentration of specific compounds in a sample by analyzing the light absorption characteristics of the sample.
Automatically generated - may contain errors
Lab products found in correlation
Endofree plasmid mega kit, by Qiagen (1 mentions)
Nanoparticle size analyzer, by Malvern Panalytical (1 mentions)
Nicolet is20 spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Geminisem 300, by Zeiss (1 mentions)
Jem f200, by JEOL (1 mentions)
Ultima 4, by Rigaku (1 mentions)
Uv 3600, by Shimadzu (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
Uv 1800, by Shimadzu (1 mentions)
S 4800, by Hitachi (1 mentions)
11 protocols using uv visible spectrometer
1
Plasmid Encoding Human IL-12 Purification
2
Antibody Immobilization on Polymer Surfaces
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PMMA, PC and COC were cut into 2 cm × 2 cm pieces and a 7 mm-diameter circular well was formed by attaching an adhesive-backed plastic stencil on the surface. Plastic pieces were exposed to UVO for 20, 40, 60 or 80 min followed by removal of the stencil. 80 μL of 1 μg mL–1 antibody solution was dispensed onto each piece and incubated for 30 min, rinsed using PBS and DI water, and dried under a stream of purified N2. This process resulted in the adsorption of enzyme-labeled antibodies on the surface (Fig. 1a ). Pieces were then rinsed using PBS and DI water to remove unabsorbed proteins (Fig. 1b ) and dried using N2. 80 μL of TMB/H2O2 was dispensed onto each sample and incubated for 1 min (Fig. 1c ) followed by absorbance measurements at 650 nm using a UV-visible spectrometer (Shimadzu, Kyoto, Japan).
3
Quantifying Total Phenolic Content
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Total phenolic content (TPC) was determined by Folin-Ciocalteu method according to the method of Haq et al. (2012) (link). The absorbance was measured at 700 nm using Shimadzu UV–Visible spectrometer and the calibration curve (Supplementary Fig. 1 ) was plotted using Gallic acid as the standard (y = 0.0078x + 0.073; R = 0.98). TPC was expressed as Gallic Acid Equivalents (GAE)/ g weight.
4
Chitosan Transparency Evaluation via UV-Vis
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Ultraviolet–visible
(UV–vis) spectroscopy was used
to investigate the transparency of original and modified chitosan
solutions. The original chitosan (1%) was dissolved in 2% acetic acid
solution and further diluted to 0.1% with water, and the modified
chitosan was also diluted to 0.1% with water. UV–vis spectra
(190–800 nm) were recorded with a UV–visible spectrometer
(Shimadzu, Japan) using quartz cuvettes.
(UV–vis) spectroscopy was used
to investigate the transparency of original and modified chitosan
solutions. The original chitosan (1%) was dissolved in 2% acetic acid
solution and further diluted to 0.1% with water, and the modified
chitosan was also diluted to 0.1% with water. UV–vis spectra
(190–800 nm) were recorded with a UV–visible spectrometer
(Shimadzu, Japan) using quartz cuvettes.
5
Multi-Technique Characterization of Nanomaterials
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The morphology of samples was observed by scanning electron microscope (ZEISS Gemini SEM 300, ZEISS, Jena, Germany) under the acceleration voltage of 5.0 kV. Transmission electron microscopy (TEM) investigations were performed by JEOL JEM-F200 (Tokyo, Japan). The phase and crystal structure of samples were analyzed by X-ray diffractometer (XRD, Rigaku Ultima IV, Los Angeles, CA, USA). Fourier transform infrared (FTIR) transmission spectra of samples were obtained on a Thermo Scientific Nicolet iS20 spectrophotometer (Waltham, MA, USA). The chemical compositions were measured via X-ray photoelectron spectroscopy (XPS, Thermo Scientific K-Alpha, Waltham, MA, USA). The hydrodynamic diameter, zeta potential, and polydisperse index of the material were analyzed by a nanoparticle size analyzer (Malvern Panalytical, Malvern, UK). The absorption spectrum of the material was examined on a UV–visible spectrometer (Shimadzu, Kyoto, Japan).
6
Bacterial Removal by Prepared Membranes
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The capability of bacteria removal by the prepared membranes was evaluated using E. coli water suspension. Optical density at 600 nm (OD600) of the bacteria suspension was about 0.12. The suspension was filtered in the dead end cell as in the case of measuring water flux mentioned above. The filtrate was collected and the turbidity of the cell suspension was examined using UV-visible spectrometer (Shimadzu, Tokyo, Japan) and expressed as OD. The capability of the prepared membranes to remove E. coli from water was calculated using the following formula:
7
Plasma Protein and Albumin Quantification
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The total protein and albumin of the plasma sample of untreated rats were measured by using the total protein and albumin assay kits (Delta Darman Part, Tehran, Iran). Briefly, the total protein determination kit relied on biuret colorimetry. Existing proteins in an alkaline medium combine with copper ions to form azure complexes, and the intensity of the color is proportional to the amount of protein in the sample. The albumin kit is based on forming blue-green complexes of this protein with bromocresol green substance in acidic environments, the color intensity of which is proportional to the sample's albumin concentration. The UV-Visible spectrometer (Shimadzu, Japan) was used to determine the absorption of each sample, and the concentrations were calculated based on the absorption of standard solutions.
8
Ferroelastic Domain Patterns and UV-Vis Spectroscopy
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The optical images of ferroelastic domain patterns were captured by Olympus BX51 microscope, and a polarizer was placed in front of CCD for polarized optical study. Solid-state diffuse reflectance UV-vis spectra were measured on UV-3600 Shimadzu UV-visible spectrometer equipped with an integrating sphere and BaSO4 as a reference sample.
9
Measuring Total Flavonoid Content
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Total flavonoid content (TFC) was determined by Aluminium chloride method as described by Chang et al. (2002) . The absorbance was measured at 405 nm using Shimadzu Uv–visible spectrometer and the calibration curve (Supplementary Fig. 2 ) was plotted using Quercetin as standard (y = 0.0056x + 0.1939; R = 0.98). TFC was expressed as Quercetin Equivalents (QE) /g weight.
10
Spectroscopic Analysis of Dye-DNA Interaction
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The UV-visible absorption spectra of dye and dye in the presence of CT-DNA were recorded in the absence and presence of HRP-A and H2O2. For UV-visible measurements absorption spectra were recorded using a UV-1800 Shimadzu UV/Visible spectrometer operating from 200 to 800 nm in 1.0 cm quartz cells. The absorbance titrations were performed at fixed dye concentration before and after decolorization while varying CT-DNA (20, 40, 60, 80, 100, 120, 140 and 160 μM final concentration).
The reaction mixtures were incubated for 90 min at 37 °C. Afterwards, the UV-Vis absorption spectra were measured against the appropriate buffer as a blank.
The reaction mixtures were incubated for 90 min at 37 °C. Afterwards, the UV-Vis absorption spectra were measured against the appropriate buffer as a blank.
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