Log In Sign up
The largest database of trusted experimental protocols

Shigella flexneri

Manufactured by American Type Culture Collection
Visit Supplier
Sourced in United States, China

Shigella flexneri is a bacterium that can be used for laboratory research purposes. It is a Gram-negative, non-spore-forming, rod-shaped pathogen that belongs to the Enterobacteriaceae family. Shigella flexneri is a key laboratory tool for studying bacterial infections and developing related diagnostic and treatment methods.

Automatically generated - may contain errors

Lab products found in correlation

Escherichia coli, by American Type Culture Collection (10 mentions) Staphylococcus aureus, by American Type Culture Collection (10 mentions) Enterococcus faecalis, by American Type Culture Collection (6 mentions) Salmonella enterica, by American Type Culture Collection (4 mentions) Bacillus cereus, by American Type Culture Collection (4 mentions) Pseudomonas aeruginosa, by American Type Culture Collection (3 mentions) Listeria monocytogenes, by American Type Culture Collection (3 mentions) Enterobacter aerogenes, by American Type Culture Collection (3 mentions) Salmonella typhimurium, by American Type Culture Collection (3 mentions) Proteus mirabilis, by American Type Culture Collection (2 mentions) Bacillus subtilis, by American Type Culture Collection (2 mentions) Staphylococcus epidermidis, by American Type Culture Collection (2 mentions) Klebsiella pneumoniae, by American Type Culture Collection (2 mentions) Shigella sonnei, by American Type Culture Collection (2 mentions) Salmonella typhi, by American Type Culture Collection (1 mentions) Micrococcus luteus, by American Type Culture Collection (1 mentions) E coli, by American Type Culture Collection (1 mentions) Bacillus megaterium, by American Type Culture Collection (1 mentions) Klebsiella oxytoca, by American Type Culture Collection (1 mentions) Staphylococcus saprophyticus, by American Type Culture Collection (1 mentions)

13 protocols using shigella flexneri

1

Antimicrobial Susceptibility Testing Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ampicillin (APC) and vancomycin (VAN) were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). Mueller-Hinton broth (MHB) medium was ordered from Hope Biotechnology Co., Ltd (Qingdao, China). Pepsin, papain, and trypsin were all sourced from Aladdin (Shanghai, China). Escherichia coli CMCC44102, Escherichia coli CMCC 44817, Escherichia coli ATCC 8739, Escherichia coli CMCC 44102, Escherichia coli ATCC 8739, Salmonella enterica CMCC 50335, Shigella flexneri CMCC 51571, Shigella sonnei CMCC 51592, Shigalla dysenteria CMCC 51252, Shigella flexneri CMCC 51572, Bacillus cereus CMCC 63301, Bacillus pumilus CMCC 63202, Staphylococcus aureus ATCC 43300, Staphylococcus aureus ATCC 29213, Staphylococcus aureus CMCC 26003, Staphylococcus aureus ATCC 12600, Staphylococcus aureus ATCC 6538, MRSA N315, and MRSA ATCC 43300 were provided by University of Science and Technology of China. Caco-2 cells were obtained from BeNa Biotechnology Co., Ltd (Beijing, China).
Chen X., Wu X, & Wang S. (2022). An optimized antimicrobial peptide analog acts as an antibiotic adjuvant to reverse methicillin-resistant Staphylococcus aureus. NPJ Science of Food, 6, 57.
+ Open protocol
+ Expand
2

Evaluating Antimicrobial Activities

Check if the same lab product or an alternative is used in the 5 most similar protocols
Standard isolates of different bacteria strains: American Type Culture Collection (ATCC)
including Staphylococcus aureus (ATCC 25923), Escherichia
coli (ATCC 25922), Salmonella typhi (ATCC 13062),
Shigella flexneri (ATCC 12022), Pseudomonas aeruginosa(ATCC 27853), and Proteus mirabilis (ATCC 29906) were obtained from the
Ethiopian Public Health Institute (Addis Ababa, Ethiopia). The bacteria were purposely
selected for evaluating the spectrum of activity and considering the folkloric repute of
the experimental plants.
Sisay M., Bussa N., Gashaw T, & Mengistu G. (2019). Investigating In Vitro Antibacterial Activities of Medicinal Plants Having Folkloric Repute in Ethiopian Traditional Medicine. Journal of Evidence-based Integrative Medicine, 24, 2515690X19886276.
+ Open protocol
+ Expand
3

Bacterial DNA Extraction and Specificity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Extracted DNA from the strains Escherichia coli K-12 MG1655 (700926), Bacillus cereus str. Frankland and Frankland (10876), Vibrio parahaemolyticus (17802D-5), and Clostridioides difficile (9689D-5) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). The additional bacteria and their sources used in specificity testing included Bacillus megaterium, Bacillus subtilis, Bacillus thuringiensis, Citrobacter freudii, Enterobacter aerogenes, E. coli (ATCC 25922), Klebsiella oxytoca, Listeria monocytogenes, Micrococcus luteus, Salmonella enterica, Serratia macrescens, Shigella flexneri, Staphylococcus capitis, and Staphylococcus saprophyticus, as listed in Table 1. B. cereus and E. coli bacterial cultures were also obtained from the Carolina Biological Supply Company (Burlington, NC, USA). E. coli and B. cereus were inoculated in Luria–Bertani (LB) medium and grown at 37 °C. A hemocytometer and a compound light microscope were used to obtain cell counts. Human HL-60 DNA was also tested in the specificity studies and was obtained from ATCC.
Bender A.C., Faulkner J.A., Tulimieri K., Boise T.H, & Elkins K.M. (2020). High Resolution Melt Assays to Detect and Identify Vibrio parahaemolyticus, Bacillus cereus, Escherichia coli, and Clostridioides difficile Bacteria. Microorganisms, 8(4), 561.
+ Open protocol
+ Expand
4

Antibacterial Activity of MPs Leaf Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibacterial activity of leaf extracts of the selected MPs was evaluated against selected standard bacterial strains of the American Type Culture Collection (ATCC). The bacterial strains selected were representative of both classes of Gram-positive and Gram-negative bacteria. The tested gram-positive bacterial strains were Staphylococcus aureus (ATCC 25923), Streptococcus agalactiae (ATCC 12386), Staphylococcus epidermidis (ATCC 12228), Enterococcus faecalis (ATCC 29212) and those of the gram-negative bacterial strains included Proteus mirabilis (ATCC 35659), Salmonella typhimurium (ATCC 13311), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922) and Shigella flexneri (ATCC 12022). The microorganisms were maintained at the Traditional and Modern Medicine Research Directorate (TMMRD) of Armauer Hansen Research Institute (AHRI) microbiology laboratory on Triptosoya + 20% glycerol broth at -78°C. Finally, the selected bacterial strains were cultivated using Mueller-Hinton broth [26 ].
Alemu M., Lulekal E., Asfaw Z., Warkineh B., Debella A., Abebe A., Degu S, & Debebe E. (2024). Antibacterial activity and phytochemical screening of traditional medicinal plants most preferred for treating infectious diseases in Habru District, North Wollo Zone, Amhara Region, Ethiopia. PLOS ONE, 19(3), e0300060.
+ Open protocol
+ Expand
5

Host Range of Phages against XDR P. aeruginosa

Check if the same lab product or an alternative is used in the 5 most similar protocols
Forty XDR P. aeruginosa clinical isolates (all resistant to GEN, AMK, CAZ, FEP, IPM, MEM, CIP and TZP) obtained from burn patients (kindly provided from Infectious and Tropical Diseases Research Center, Tabriz, Iran) were used to determine host range of isolated phages. Sensitivity of bacterial strains was assessed by spot test. In summary 2 - 3 colony of isolated strains dissolved in LB broth (5 mL) and incubated overnight at 37 °C with shaking (120 RPM), then 100 µl of bacterial culture was added to 5 mL of soft LB agar (45°C, 0.7% agar) and overlaid onto plates containing 1.5% LB agar after mixing. After solidification (10 minutes), 10 µl of isolated phages were spotted on the top layer. The plates were incubated overnight at 37°C until the formation of lysis zones. Lytic activity of extracted phages was also tested on Acinetobacter baumannii (ATCC 19606) (ATCC, Manassas, VA, USA), Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC2392), Enterococcus faecalis (ATCC29212), Shigella flexneri (ATCC12022).
Marashi S.M., Nikkhahi F., Hamedi D, & Shahbazi G. (2022). Isolation, Characterization and in vitro Evaluation of Specific Bacteriophages Targeting Extensive Drug Resistance Strains of Pseudomonas aeruginosa Isolated from Septic Burn Wounds. Infection & Chemotherapy, 54(1), 153-164.
+ Open protocol
+ Expand
6

Antibacterial Potential of A. tribuloides

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibacterial activity of the A. tribuloides root extract and greenly synthesized AgNPs was investigated on four bacteria strains, namely Bacillus cereus (ATCC 11778), Staphylococcus aureus (ATCC 25923), Shigella flexneri (ATCC 12022), and Escherichia coli (ATCC 25922). The bacteria were obtained from Iranian Research Organization for Science and Technology (IROST).
Sharifi-Rad M., Pohl P., Epifano F, & Álvarez-Suarez J.M. (2020). Green Synthesis of Silver Nanoparticles Using Astragalus tribuloides Delile. Root Extract: Characterization, Antioxidant, Antibacterial, and Anti-Inflammatory Activities. Nanomaterials, 10(12), 2383.
+ Open protocol
+ Expand
7

Microbial Strain Collection and Culture Conditions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reference microbial strains were obtained from the American Type Culture Collection (ATCC), including Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6635, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27833 and Shigella flexneri ATCC 12022. The Listeria monocytogenes PCM 2191 and Bacillus cereus PCM 1948 strains were taken from the Polish Collection of Microorganisms (PCM). One bacterial strain Salmonella Enteritidis ZMF 279 was derived from the collection of the Department of Pharmaceutical Microbiology and Diagnostic Microbiology, Medical University of Lodz. Two fungal strains were also used: Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404. All tested microorganisms were stored at –80 °C in 15% glycerol stocks. Before the investigation, the bacterial strains were transferred to Mueller-Hinton agar medium (Oxoid, Thermo Fisher Scientific, Waltham, MA, USA) and cultured overnight at 37 °C. Fungal strains were transferred on Sabouraud agar medium (Oxoid, Thermo Fisher Scientific, Waltham, MA, USA) and cultured for two days at 30 °C.
Sikorska-Zimny K., Lisiecki P., Gonciarz W., Szemraj M., Ambroziak M., Suska O., Turkot O., Stanowska M., Rutkowski K.P., Chmiela M, & Mielicki W. (2021). Influence of Agronomic Practice on Total Phenols, Carotenoids, Chlorophylls Content, and Biological Activities in Dry Herbs Water Macerates. Molecules, 26(4), 1047.
+ Open protocol
+ Expand
8

Antibacterial and Antifungal Efficacy of TPEO

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The microbial strains used to determine the TPEO antibacterial and antifungal activity were reference strains: Escherichia coli ATCC 25922, Shigella flexneri ATCC 12022, Streptococcus pyogenes ATCC 19615, Salmonella typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 10231 and Candida parapsilosis ATCC 22019 (Microbiologics, Molsheim, France). According to the EFSA One Health 2020 Zoonoses Report [36 (link)], these strains generated the majority of foodborne outbreaks across the EU member states.
Jianu C., Rusu L.C., Muntean I., Cocan I., Lukinich-Gruia A.T., Goleț I., Horhat D., Mioc M., Mioc A., Șoica C., Bujancă G., Ilie A.C, & Muntean D. (2022). In Vitro and In Silico Evaluation of the Antimicrobial and Antioxidant Potential of Thymus pulegioides Essential Oil. Antioxidants, 11(12), 2472.
+ Open protocol
+ Expand
9

Antibacterial Screening of Metal Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
All metal salts, ligands, metal complexes, AgNPs and AgNPs-complexes were screened in vitro for their antibacterial activities against four Gram-positive (Staphylococcus aureus (ATCC 22923), Methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecalis (ATCC 19433) and Bacillus cereus (ATCC 14579)) and four Gram-negative (Escherichia coli (ATCC 25922), Enterobacter aerogenes (ATCC 13048), Salmonella typhimurium (ATCC 14028) and Shigella flexneri (ATCC 29903)) bacterial strains. All strains used were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and stored as glycerol stocks in the Laboratory of Mycology and Phytopathology, at Universidad de los Andes, Bogotá D.C.
Bello-Vieda N.J., Pastrana H.F., Garavito M.F., Ávila A.G., Celis A.M., Muñoz-Castro A., Restrepo S, & Hurtado J.J. (2018). Antibacterial Activities of Azole Complexes Combined with Silver Nanoparticles. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(2), 361.
+ Open protocol
+ Expand
10

Antibacterial Activity of T. polium Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibacterial activity of the different extracts of the T. polium material plant was investigated using Gram-positive (Bacillus cereus (ATCC 8035) and Staphylococcus aureus (ATCC 25923)) bacteria strains and Gram-negative (Shigella flexneri (ATCC 12022) and Escherichia coli (ATCC 25922)) bacteria strains. The bacteria strains were obtained from the Iranian Research Organization for Science and Technology (IROST).
Sharifi-Rad M., Pohl P., Epifano F., Zengin G., Jaradat N, & Messaoudi M. (2022). Teucrium polium (L.): Phytochemical Screening and Biological Activities at Different Phenological Stages. Molecules, 27(5), 1561.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!