Thy1 yfp h
Thy1-YFP-H is a transgenic mouse line expressing yellow fluorescent protein (YFP) under the control of the Thy1 promoter. Thy1 is a cell surface glycoprotein expressed in a subset of neurons, allowing for the visualization of neuronal morphology and connectivity in this mouse model.
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11 protocols using thy1 yfp h
Transgenic Mouse Models for Neuroscience
Sciatic Nerve Injury Model in Thy1-YFP Mice
The surgical procedure was carried out following sterile precautions. Mice were anesthetized with ketamine-xylazine (90–10 mg/kg) and the right sciatic nerve at the mid thigh level was exposed. Treatments were performed by direct injections at 45 mm from the tip of the third digit in 2 μl of sterile PBS, using a fine glass micropipette connected to a Hamilton syringe. Immediately after the injection and at the same position, the nerve was crushed in two different directions 30 s each time with fine forceps. The crush site was labeled with lamp black powder. The wound was closed with 5–0 mononylon Ethilon sutures (Ethicon) and disinfected. The sciatic and tibial nerves and the plantar skin were harvested at 24 h, 3, 10, and 28 days post lesion (dpl).
All nerve injections were performed in 2 μl PBS and at 10 μg/ml concentration of the following products: rCD300f-IgG2a (rat extracellular domain of CD300f fused to mouse IgG2a protein) or purified mouse myeloma IgG2a (Invitrogen, Cat. N° 026200).
Generation of Transgenic Mouse Models
Transgenic Mouse Model Experiments
Transgenic mouse models for neural circuit analysis
Visualizing Neuronal Morphology in TDP-43 Mice
Thy1-YFP-H Mouse Model Study
Conditional Knockout of Kif5b in Layer V Neurons
Fluorescent Mouse Lines for Neuroscience
Mouse and Zebrafish Experiments Protocol
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