Positively charged slides

Manufactured by Avantor

Sourced in United States

Positively charged slides are laboratory equipment designed to facilitate the attachment and immobilization of negatively charged biological samples, such as DNA, RNA, or proteins, onto the slide surface. The positive charge on the slide surface electrostatically attracts and binds the negatively charged samples, enabling various downstream applications in research and diagnostics.

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Lab products found in correlation

Eosin y, by Avantor (7 mentions) Hematoxylin qs, by Vector Laboratories (7 mentions) Bx40 microscope, by Olympus (7 mentions) Dp25 imaging system, by Olympus (6 mentions) Cytoseal xyl mounting media, by Thermo Fisher Scientific (4 mentions) Vectamount mounting media, by Vector Laboratories (3 mentions) Fluorometric activity assay, by Merck Group (2 mentions) Total bilirubin elisa, by Cusabio (2 mentions) Catalyst one serum chemistry analyzer, by IDEXX (2 mentions) Tryptase, by Thermo Fisher Scientific (1 mentions) Fluorescence microscope, by Zeiss (1 mentions) Antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Cytospin 4, by Thermo Fisher Scientific (1 mentions) Cd45.1 a20, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Positively charged glass slides Positively charged microscope slides

10 protocols using positively charged slides

Histological and Biochemical Analysis of Liver in AOM-Treated Mice

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Paraffin-embedded livers from vehicle and AOM-treated mice were sectioned into 3 µm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) for one minute followed by staining for one minute with eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through 2 xylene washes. Coverslips were mounted onto the slides using Vectamount mounting media (Vector Laboratories). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA).
Serum ALT and bilirubin were assessed using commercially available kits. Alanine aminotransferase measurement was performed using a fluorometric activity assay (Sigma-Aldrich, St. Louis, MO). Total bilirubin was assayed using a total bilirubin ELISA (CusaBio, Wuha, China). All assays and subsequent analyses were performed according to the manufacturer’s instructions.

McMillin M., Frampton G., Tobin R., Dusio G., Smith J., Shin H., Newell-Rogers M.K., Grant S, & DeMorrow S. (2015). TGR5 signaling reduces neuroinflammation during hepatic encephalopathy. Journal of neurochemistry, 135(3), 565-576.

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Histological Analysis of Liver Tissue

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Paraffin-embedded livers were cut into 4 μm sections and mounted onto positively charged slides (VWR, Radnor, PA, USA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA, USA) for 1 min followed by staining for 1 min with eosin Y (Amresco, Solon, OH, USA) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (ThermoFisher). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA, USA). Serum alanine aminotransferase (ALT) concentrations were measured using a commercially available kit from Sigma-Aldrich with all assays and subsequent analyses performed according to the instructions provided by the manufacturer.

McMillin M., Frampton G., Grant S., Khan S., Diocares J., Petrescu A., Wyatt A., Kain J., Jefferson B, & DeMorrow S. (2017). Bile Acid-Mediated Sphingosine-1-Phosphate Receptor 2 Signaling Promotes Neuroinflammation during Hepatic Encephalopathy in Mice. Frontiers in Cellular Neuroscience, 11, 191.

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Histological Analysis of Paraffin-Embedded Liver Sections

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Paraffin-embedded livers were cut into 4-μm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) followed by staining with eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (ThermoFisher). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA). All images are taken as × 200 magnification.
Liver function was assessed by measuring serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels using a Catalyst One serum chemistry analyzer from IDEXX Laboratories, Inc. (Westbrook, MA).

McMillin M., Grant S., Frampton G., Petrescu A.D., Williams E., Jefferson B., Thomas A., Brahmaroutu A, & DeMorrow S. (2019). Elevated circulating TGFβ1 during acute liver failure activates TGFβR2 on cortical neurons and exacerbates neuroinflammation and hepatic encephalopathy in mice. Journal of Neuroinflammation, 16, 69.

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Micronuclei Assay Protocol for Cell Evaluation

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For the micronuclei assay, cells were dissociated into single cells using tryptase (Gibco, 10 min). The cells were harvested and resuspended in PBS-EDTA (1 mM EDTA). The cell count was adjusted to 1 million cells/ml, and cells were harvested (∼50–100 µl/ slide) by centrifugation on positively charged slides (VWR) using CytoSpin 4 (Thermo Scientific). The centrifugation was done at 500 rpm for 5 min. The slides were air dried and mounted with Antifade Mountant with DAPI (Invitrogen). The slides were allowed to dry before scoring for micronuclei. The slides were read on Zeiss fluorescence microscopes at 100X magnification. The nuclei were assessed for the presence of micronuclei, and micronuclei bridges. All results were performed in triplicates, and ≥120 cells were scored for each experiment. We used Student’s t test with two-tailed distribution for P value calculation.

Paniza T., Deshpande M., Wang N., O’Neil R., Zuccaro M.V., Smith M.E., Madireddy A., James D., Ecker J., Rosenwaks Z., Egli D, & Gerhardt J. (2020). Pluripotent stem cells with low differentiation potential contain incompletely reprogrammed DNA replication. The Journal of Cell Biology, 219(9), e201909163.

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Histological Assessment of Liver Damage

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Liver damage was assessed by H&E staining according to previously published protocols.⁷ (link) Paraffin-embedded livers were cut into 4-μm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories) for 1 minute followed by staining for 1 minute with eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides then were dipped into 100% ethanol and subsequently through 2 xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (Thermo Fisher Scientific, Waltham, MA). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Center Valley, PA).
Liver function was assessed by measuring plasma alanine aminotransferase and aspartate aminotransferase using the IDEXX Catalyst One machine from IDEXX Laboratories, Inc.

McMillin M., Grant S., Frampton G., Petrescu A.D., Kain J., Williams E., Haines R., Canady L, & DeMorrow S. (2018). FXR-Mediated Cortical Cholesterol Accumulation Contributes to the Pathogenesis of Type A Hepatic Encephalopathy. Cellular and Molecular Gastroenterology and Hepatology, 6(1), 47-63.

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Histological Scoring of Organ Tissues

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The tissues fixed in 10% neutral buffered formalin were submitted to the Diagnostic Services Unit (DSU) at the University of Calgary, Faculty of Veterinary Medicine for obtaining paraffin embedded tissue blocks and hematoxylin-eosin (H&E) stained and unstained tissue sections on positively-charged slides (VWR International, Radnor, PA, USA). The stained tissue sections were examined under the microscope (Olympus BX51, Center Valley, PA, USA) and scored following the methods previously described with some alteration [24 (link)]. There were several criteria for scoring each organ (Table 1), and the scoring system was performed as follows: normal (0), mild (1), moderate (2), and severe (3) for each criterion, and then the total score was calculated for each organ per group.

Isham I.M., Hassan M.S., Abd-Elsalam R.M., Ranaweera H.A., Mahmoud M.E., Najimudeen S.M., Ghaffar A., Cork S.C., Gupta A, & Abdul-Careem M.F. (2023). Impact of Maternal Antibodies on Infectious Bronchitis Virus (IBV) Infection in Primary and Secondary Lymphoid Organs of Chickens. Vaccines, 11(7), 1216.

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Histological Liver Analysis and Serum Biomarkers

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Paraffin-embedded livers were cut into 3-μm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) for 1 min, followed by staining for 1 min with eosin Y (Amresco, Solon, OH), and rinsed in 95 % ethanol. The slides were then dipped into 100 % ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using Vectamount mounting media (Vector Laboratories). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA).
Serum alanine aminotransferasase (ALT) and bilirubin were assessed using commercially available kits. Alanine aminotransferase measurement was performed using a fluorimetric activity assay. Total bilirubin was assayed using a total bilirubin ELISA. All assays and subsequent analyses were performed according to the instructions provided by the manufacturers.

McMillin M., Grant S., Frampton G., Andry S., Brown A, & DeMorrow S. (2016). Fractalkine suppression during hepatic encephalopathy promotes neuroinflammation in mice. Journal of Neuroinflammation, 13(1), 198.

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Histochemical and Biochemical Analysis of Liver Function

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Paraffin-embedded livers were sectioned into 3 μm sections and mounted onto positively charged slides (VWR, Radnor, PA, USA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA, USA) for 1 min followed by staining for 1 min with eosin Y (Amresco, Solon, OH, USA) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using Vectamount mounting media (Vector Laboratories). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA, USA).
Serum alanine aminotransferase (ALT) and bilirubin were assessed using commercially available kits. ALT measurement was performed using a fluorometric activity assay (Sigma-Aldrich). Total bilirubin was assayed using a total bilirubin ELISA (CusaBio, Wuha, China). All assays and subsequent analyses were performed according to manufacturers’ instructions.

McMillin M., Frampton G., Thompson M., Galindo C., Standeford H., Whittington E., Alpini G, & DeMorrow S. (2014). Neuronal CCL2 is upregulated during hepatic encephalopathy and contributes to microglia activation and neurological decline. Journal of Neuroinflammation, 11, 121.

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Quantification of Liver Necrosis and Function

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Paraffin-embedded livers were cut into 4 μm sections and mounted onto positively-charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) followed by staining with Eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through 2 xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (ThermoFisher Scientific). The slides were viewed and imaged using an Olympus BX40 microscope with a DP25 imaging system (Olympus, Center Valley, PA). Percentage area of necrosis was calculated using 20× field views, drawing areas devoid of hepatocyte nuclei and calculating area using ImageJ software (National Institutes of Health, Bethesda, MD). In each mouse, the necrosis from 5 to 10 fields were quantified and averaged together to determine necrotic area.
Liver function was assessed by measuring serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels using a Catalyst One serum chemistry analyzer from IDEXX Laboratories, Inc. (Westbrook, MA). For control groups, serum was diluted 1:2 in deionized H₂0 and for APAP-treated groups, serum was diluted 1:9 in deionized H₂0 prior to running on the analyzer.

Frampton G., Reddy P., Jefferson B., Ali M., Khan D, & McMillin M. (2020). Inhibition of thrombospondin-1 reduces glutathione activity and worsens acute liver injury during acetaminophen hepatotoxicity in mice. Toxicology and applied pharmacology, 409, 115323.

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Immunohistochemical Analysis of Brain Tissue

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In all cases, histological analysis was performed on fresh-frozen brain tissue embedded in optimum cutting temperature (OCT) medium. The tissue was cryo-sectioned (7-8 mm) and mounted onto positively charged slides (VWR). The primary antibodies used for IF were as follows: CD4 (RM4-5), B220 (RA3-6B2), CD11c (N418), CD45.1 (A20), CD45.2 (104) TCRb (H57-597), PDGFRa (APA5), and PDGFRb (APB5), all of which are from eBioscience; fibronectin (GW20021F, Sigma); smooth muscle actin (1A4, Sigma), and ERTR7 (T-2109, BMA Biomedical).

Pikor N.B., Astarita J.L., Summers-Deluca L., Galicia G., Qu J., Ward L.A., Armstrong S., Dominguez C.X., Malhotra D., Heiden B., Kay R., Castanov V., Touil H., Boon L., O'Connor P., Bar-Or A., Prat A., Ramaglia V., Ludwin S., Turley S.J, & Gommerman J.L. (2015). Integration of Th17- and Lymphotoxin-Derived Signals Initiates Meningeal-Resident Stromal Cell Remodeling to Propagate Neuroinflammation. Immunity, 43(6).

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