Ftir 8400s instrument

All reactions in this study were conducted under an argon atmosphere (G2 grade (purity > 99.9995%, JAPAN FINE PRODUCTS (JFP), Kawasaki, Kanagawa, Japan) and stirred using Magnetic stirrer (PTFE stirrer, football type, As one, Osaka, Japan). All substrates were purchased from Tokyo Chemical Industry Co., Ltd., (Kawaguchi, Saitama, Japan) and used as received. The Janus precursor [ViSi(OSiMe₂H)O]₄ and potassium all-cis-tetravinylcyclotetrasiloxanolate were stored under anhydrous and argon atmospheres. All solvents were distilled and stored on anhydrous molecular sieves (Wako Pure Chemical Industries, Ltd., Osaka, Japan). Catalyst B(C₆F₅)₃ was stored under an argon atmosphere. LC-5000 recycle-type preparative liquid chromatography was performed using a combination of a JAIGEL 1HR + 2HR (20 mm × 600 mm) GPC column (Japan Analytical Industry Co., Ltd., Tokyo, Japan) (eluent: CHCl₃,). Fourier-transform NMR spectra were obtained on a JEOL JNM-ECS 600 NMR spectrometer (JEOL Ltd., Akishima, Tokyo, Japan), ¹H at 600 MHz, ¹³C at 150.91 MHz, and ²⁹Si at 119.24 MHz). MALDI-TOF mass spectrometry was performed on a Shimadzu MALDI-TOF AXIMA^® instrument (Shimadzu Corporation, Kyoto, Japan). IR spectra were measured with a Shimadzu FTIR-8400S instrument (Shimadzu Corporation, Kyoto, Japan).

The chemical composition of clary sage and black pepper essential oil was characterised by Gas Chromatography-Mass Spectroscopy (GC-MS, Varian, Santa Clara, CA, USA). The essential oils were diluted 25 times with ethyl acetate and analysed using a Hewlett Packard mass detector and a HP-5MS column (length 30 m, inner diameter 0.25 mm, film thickness 0.25 μm). The injector, GC-MS interphase, ion source and selective mass detector temperature were maintained at 250, 280, 250 and 150 °C. The oven temperature for both oils was programmed as follows: 60 °C (1 min), 60–185 °C (1.5 °C/min), 185 °C (1 min), 185–275 °C (9 °C/min), 275 °C (2 min). Split injection was performed with helium as carrier gas, with a flow rate of 1.1602 mL/min. The split ratio of the column was fixed at 40:1. The pressure of the column was set at 9.4 psi. The components were identified by comparing the mass spectra obtained with mass spectra of standards obtained with the same column.
Fourier Transform Infrared Spectroscopy (FTIR) analysis was carried out using Attenuated Total Reflection Infrared (ATR-IR) technique (FTIR-8400S instrument, Shimadzu, Columbia, DC, USA). The range of scan was 600–2500 cm⁻¹ with 64 scans and resolution of 4 cm⁻¹.

Infrared spectra of rivastigmine tartrate alone and when combined with excipients (i.e., lipid, surfactant, physical mixture, and formulation) were determined with FTIR-8400S instrument (Shimadzu Corporation) using previously reported KBr disk/pellet dispersion method (Cavalli et al., 1995 ). The samples for the testing process were made into compressed pellets by first grinding them with KBr powder (Seyed et al., 2017 ). The FTIR spectra were scanned across a range of 4,000–400 cm⁻¹ (resolution 4 cm⁻¹ /50 scans). The peaks were examined for any significant differences in the spectrum obtained for the plain drug, which included RT, GMS, Polysorbate 80, RT, and GMS, and a physical mixing of RT and GMS.