Antibodies used for Western blot assay were as follows: anti-AR rabbit polyclonal antibody (N-20, Cat#sc-816, Santa Cruz Biotechnology, Santa Cruz, CA); anti-PMEPA1 mouse monoclonal antibody (2A12, Cat#H00056937-M01, ABNOVA, Taiwan); anti-NEDD4 rabbit polyclonal antibody (Cat#07–049, Millipore, Billerica, MA); anti-PTEN mouse monoclonal antibody (Cat#MS-1601-S0, Lab Vision, Fremont, CA), anti-PSA rabbit polyclonal antibody (Cat#A05662, Dako, Denmark); anti-GAPDH polyclonal antibody (FL335, Cat#sc-25778, Santa Cruz Biotechnology, Santa Cruz, CA); and horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse TrueBlot antibodies (Cat#18–8816, Cat#18–8817-33, eBioscience, San Diego, CA). Antibodies for immunohistochemistry (IHC) assay were: anti-PMEPA1 antibody mentioned above, anti-AR mouse monoclonal antibody (M3562, DAKO, Denmark), anti-PSA rabbit polyclonal antibody (Cat#A0562, DAKO, Denmark), biotinylated horse anti-mouse antibody (Cat# BA-2000, Vector, Burlingame, CA), biotinylated goat anti-rabbit antibody (Cat# BA-1000, Vector, Burlingame, CA) and VIP peroxidase (HRP) substrate kit (Cat# SK-4600, Vector, Burlingame, CA).
Li H., Mohamed A.A., Sharad S., Umeda E., Song Y., Young D., Petrovics G., McLeod D.G., Sesterhenn I.A., Sreenath T., Dobi A, & Srivastava S. (2015). Silencing of PMEPA1 accelerates the growth of prostate cancer cells through AR, NEDD4 and PTEN. Oncotarget, 6(17), 15137-15149.
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