Anti mouse cd25

Manufactured by BioXCell

Anti-mouse CD25 is a monoclonal antibody that binds to the CD25 antigen on the surface of mouse cells. CD25 is the alpha subunit of the interleukin-2 receptor and is expressed on activated T cells, regulatory T cells, and other immune cells. The primary function of this product is to detect and quantify CD25-expressing cells in mouse samples.

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Lab products found in correlation

Anti mouse ifn γ, by BioXCell (2 mentions) Anti mouse cd11c, by BioXCell (2 mentions) Anti mouse cd8, by BioXCell (2 mentions) Armenian hamster igg, by BioXCell (2 mentions) Rat igg1, by BioXCell (2 mentions) Pc 61, by BioXCell (1 mentions) Clone xmg1, by BioXCell (1 mentions) Anti mouse cd4, by BioXCell (1 mentions) Clone pc 61, by BioXCell (1 mentions) Clone gk1, by BioXCell (1 mentions) Rat igg2a, by BioXCell (1 mentions) Cell counting kit 8 (cck8), by Merck Group (1 mentions) Temsirolimus, by Merck Group (1 mentions)

Spelling variants of this product

Anti-mouse CD25 antibody (PC-61.5.3) (2 citations) Anti-mouse CD25 mAb (2 citations) Anti-mouse CD25 (PC-61.5.3) (2 citations)

4 protocols using anti mouse cd25

Treg Cell Ablation and Depletion Protocol

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For Treg cell ablation studies, DT (Sigma-Aldrich) was injected
intraperitoneally (i.p.) at 50 µg per kg of body weight at the indicated
times. For neutralization and depletion studies, anti-mouse IFN-γ (1 mg,
clone XMG1.2, Bio X Cell) or anti-mouse CD11c (500 µg, clone N418, Bio X
Cell) or anti-mouse CD8 (250 µg, clone 53–6.72, Bio X Cell) or
anti-mouse CD4 (150 µg, clone GK1.5, Bio X Cell) or anti-mouse CD25 (500
µg, clone PC-61.5.3, Bio X Cell) were injected i.p. at the indicated
times. Isotype control antibodies used were Rat IgG1 or Armenian hamster IgG or
Rat IgG2a from Bio X Cell, respectively.

Jang J.E., Hajdu C.H., Liot C., Miller G., Dustin M.L, & Bar-Sagi D. (2017). Crosstalk between Regulatory T Cells and Tumor-Associated Dendritic Cells Negates Anti-tumor Immunity in Pancreatic Cancer. Cell Reports, 20(3), 558-571.

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Treg Cell Ablation and Depletion Protocols

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For Treg cell ablation studies, DT (Sigma-Aldrich) was injected intraperitoneally (i.p.) at 50 μg per kg of body weight at the indicated times. For neutralization and depletion studies, anti-mouse IFN-γ (1 mg, clone XMG1.2, Bio X Cell), anti-mouse CD11c (500 μg, clone N418, Bio X Cell), anti-mouse CD8 (250 μg, clone 53-6.72, Bio X Cell), anti-mouse CD4 (150 μg, clone GK1.5, Bio X Cell), or anti-mouse CD25 (500 μg, clone PC-61.5.3, Bio X Cell) was injected i.p. at the indicated times. Isotype control antibodies used were rat IgG1, Armenian hamster IgG, or rat IgG2a from Bio X Cell, respectively.

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Modulating PGE2 and Treg in Obesity

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PGE₂ was dissolved in 100% DMSO and diluted in 0.9% sodium chloride to reach the concentration of 5 μg/mL. We fed 8-week old COX-2–KO mice an HFD for 8 weeks and then randomly grouped the mice into Ad and IF groups. Two weeks after IF, mice were administered 50 μg/kg PGE₂ via i.p. injection every other day for 2 weeks. For the neutralization of CD25 to block Treg function, 8-week-old C57BL/6 mice were fed an HFD for 8 weeks and then administered neutralizing antibody anti-mouse CD25 (IL-2Ra) or IgG1 isotype control anti-trinitrophenol (Bio X Cell) via i.p. injection every other day for 2 weeks starting 2 days before PGE₂ treatment. The mice were then euthanized, and tissue samples from epididymal and inguinal fat were collected for flow cytometry and RT-PCR analyses.

Wang C., Zhang X., Luo L., Luo Y., Yang X., Ding X., Wang L., Le H., Feldman L.E., Men X., Yan C., Huang W., Feng Y., Liu F., Yang X.O, & Liu M. (2022). Adipocyte-derived PGE2 is required for intermittent fasting–induced Treg proliferation and improvement of insulin sensitivity. JCI Insight, 7(5), e153755.

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Measuring Cell Proliferation Assays

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Suspension cell (EL4, Jurkat, NM001) proliferation was monitored by the colorimetric water-soluble tetrazolium salt (CCK8) assay using a Cell Counting Kit-8 (Sigma Aldrich) according to the manufacturer’s instructions. Adherent cell proliferation was measured by MTT assay. EL4 (1000–2000 cells/well), B16F10 (500–1000 cells/well), Jurkat or NM001 B cell lymphoma (20,000–40,000 cells/well) cells were seeded in triplicate into 96-well plates and treated with rapamycin alone (0.625–80 nM) or rapamycin (100–200 pg/ml) ± DD (250 ng/ml) and blocking anti-mouse CD25 (10 μg/ml) (BioXCell) and anti-mouse CD122 (10 μg/ml) (BioLegend) antibodies or temsirolimus (200–500 pg/ml) (Sigma Aldrich). After specified incubation times, 5 mg/ml MTT or CCK8 solution was added and optical density was measured at 570 nm for MTT or 450 nm for CCK8 in a microplate reader. Absolute numbers of live cells were counted in triplicate on a hemocytometer using Trypan blue.

Liu Y., Pandeswara S., Dao V., Padrón Á., Drerup J.M., Lao S., Liu A., Hurez V, & Curiel T.J. (2016). Biphasic rapamycin effects in lymphoma and carcinoma treatment. Cancer research, 77(2), 520-531.

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