0.05 m cacl2

Manufactured by AppliChem

Sourced in Germany

0.05 M CaCl2 is a laboratory reagent. It is an aqueous solution with a concentration of 0.05 moles of calcium chloride per liter.

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Lab products found in correlation

Proteose peptone, by BD (4 mentions) 0.25 m na2hpo4 7h2o, by Carl Roth (4 mentions) 0.25 m kh2po4, by Carl Roth (4 mentions) Yeast extract, by BD (4 mentions) 0.4 m mgso4 7h2o, by AppliChem (3 mentions) 2 m glucose, by Merck Group (3 mentions) Fe nh4 2 so4 2 6h2o, by AppliChem (2 mentions) Tissue culture bottles, by Sarstedt (1 mentions)

Close spelling variants of this product

CaCl2

4 protocols using 0.05 m cacl2

Cultivation of Acanthamoeba castellanii Trophozoites

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Acanthamoeba castellanii trophozoites
were cultured at room temperature in peptone yeast
glucose (PYG) 712 medium (20 g proteose peptone (BD, Sparks, USA),
1 g of yeast extract (BD, Sparks, USA), 950 mL of distilled water,
10 mL of 0.4 M MgSO₄·7H₂O (AppliChem, Darmstadt,
Germany), 8 mL of 0.05 M CaCl₂ (AppliChem, Darmstadt, Germany),
34 mL of 0.1 M sodium citrate·2H₂O (Merck, Darmstadt,
Germany), 10 mL of 0.005 M Fe(NH₄)2(SO₄)₂·6H₂O (AppliChem, Darmstadt, Germany), 10
mL of 0.25 M Na₂HPO₄·7H₂O (Roth,
Karlsruhe, Germany), 10 mL of 0.25 M KH₂PO₄ (Roth,
Karlsruhe, Germany), and 50 mL of 2 M glucose (Sigma–Aldrich
Chemie GmbH, Steinheim, Germany)). The PYG medium was exchanged at
least once a week to avoid cyst formation. Acanthamoebae were detached from the culture flask by slight knocking, collected
with a pipet and centrifuged. The generated pellet was resuspended
in PYG medium and the cell number was counted using a Neubauer counting
chamber.

Gutekunst S.B., Siemsen K., Huth S., Möhring A., Hesseler B., Timmermann M., Paulowicz I., Mishra Y.K., Siebert L., Adelung R, & Selhuber-Unkel C. (2019). 3D Hydrogels Containing Interconnected Microchannels of Subcellular Size for Capturing Human Pathogenic Acanthamoeba Castellanii. ACS Biomaterials Science & Engineering, 5(4), 1784-1792.

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Cultivation of Acanthamoeba spp. in PYG Medium

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A. castellanii (ATTC^® 30234, derived from ATCC^® 30011) and A. comandoni (Pb30/40, group 1, genotype T7 [7 ]) were cultured at room temperature in tissue culture bottles (75 ml, Sarstedt AG & Co., Nümbrecht, Germany). A Peptone Yeast Glucose (PYG) Medium 712 consisting of 20 g proteose peptone (BD, Sparks, USA), 1 g yeast extract (BD, Sparks, USA), 950 ml distilled water, 8 ml 0.05 M CaCl₂ (AppliChem GmbH, Darmstadt, Germany), 10 ml 0.4 M MgSO₄ × 7H₂O (AppliChem GmbH, Darmstadt, Germany), 10 ml 0.25 M Na₂HPO₄ × 7H₂O (Carl Roth GmbH & Co. KG, Karlsruhe, Germany), 10 ml 0.25 M KH₂PO₄ (Carl Roth GmbH & Co. KG, Karlsruhe, Germany), 34 ml 0.1 M Na citrate × 2H₂O (Merck KGaA, Darmstadt, Germany), 10 ml 0.005 M Fe(NH₄)₂(SO₄)₂ × 6H₂O (AppliChem GmbH, Darmstadt, Germany), and 50 ml 2 M glucose (Sigma-Aldrich Chemie GmbH, Munich, Germany)) was used. The medium was renewed once a week by shaking the bottles, removing the old medium with swimming acanthamoebae, and adding fresh medium to the remaining cells.

Huth S., Reverey J.F., Leippe M, & Selhuber-Unkel C. (2017). Adhesion forces and mechanics in mannose-mediated acanthamoeba interactions. PLoS ONE, 12(5), e0176207.

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Culturing A. castellanii Trophozoites in PYG Medium

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Trophozoites of A. castellanii (ATTC 30234) were cultured at room temperature in Peptone Yeast Glucose (PYG) 712 medium (20.0 g proteose peptone (BD, Sparks, USA), 1.00 g yeast extract (BD, Sparks, USA), 950 mL distilled water, 10.0 mL 0.4 M MgSO₄·7H₂O (AppliChem, Darmstadt, Germany), 8.00 mL 0.05 M CaCl₂ (AppliChem, Darmstadt, Germany), 34.0 mL 0.1 M sodium citrate dihydrate (Merck, Darmstadt, Germany), 10.0 mL 0.005 M (NH₄)₂Fe(SO₄)₂·6H₂O (AppliChem, Darmstadt, Germany), 10.0 mL 0.25 M Na₂HPO₄·7H₂O (Roth, Karlsruhe, Germany), 10.0 mL 0.25 M KH₂PO₄ (Roth, Karlsruhe, Germany), 50.0 mL 2 M glucose (Sigma–Aldrich Chemie GmbH, Steinheim, Germany)). In this axenic culture, the PYG 712 medium was regularly exchanged in the cell culture flasks in order to avoid an encystment of A. castellanii trophozoites.

Gutekunst S.B., Grabosch C., Kovalev A., Gorb S.N, & Selhuber-Unkel C. (2014). Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii. Beilstein Journal of Nanotechnology, 5, 1393-1398.

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Culturing Acanthamoeba castellanii Trophozoites

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Example 1

Acanthamoeba were cultured according to Gutekunst et al. (Beilstein J Nanotechnol. 2014, 5, 1393-1398). In brief, trophozoites of Acanthamoeba castellanii (A. castellanii, ATTC 30234) were cultured at room temperature in Peptone Yeast Glucose (PYG) 712 medium (20.0 g proteose peptone (BD, Sparks, USA), 1.00 g yeast extract (BD, Sparks, USA), 950 mL dist. H₂O, 10.0 mL 0.40 M MgSO₄.7H₂O (AppliChem, Darmstadt, Germany), 8.00 mL 0.05 M CaCl₂(AppliChem, Darmstadt, Germany), 34.0 mL 0.10 M sodium citrate.2H₂O (Merck, Darmstadt, Germany), 10.0 mL 5.00 mM Fe(NH₄)₂(SO₄)2.6H₂O (AppliChem, Darmstadt, Germany), 10.0 mL 0.25 M Na₂HPO₄.7H₂O (Roth, Karlsruhe, Germany), 10.0 mL 0.25 M KH₂PO₄(Roth, Karlsruhe, Germany), 50.0 mL 2.00 M glucose (Sigma-Aldrich Chemie GmbH, Steinheim, Germany)). In this axenic culture, the PYG 712 medium was regularly exchanged in the cell culture flasks in order to avoid encystment of A. castellanii trophozoites.

US10688469B2. Microporous hydrogels (2020-06-23). Christian-Albrechts-Universität zu Kiel [DE]. Inventors: Christine Selhuber-Unkel [DE], Iris Hölken [DE], Sören Björn Gutekunst [DE], Rainer Adelung [DE].

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