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Human duoset elisa development kit

Manufactured by R&D Systems
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The Human Duoset ELISA development kit is a set of reagents designed for the development of sandwich enzyme-linked immunosorbent assay (ELISA) to quantify human protein targets in biological samples. The kit includes a matched antibody pair and other necessary components to establish an ELISA protocol.

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Lab products found in correlation

Spectramax m2e, by Molecular Devices (1 mentions) Fractalkine, by Eve Technologies (1 mentions) Tnf α, by Eve Technologies (1 mentions) Mip 1α, by Eve Technologies (1 mentions) Rantes, by Eve Technologies (1 mentions) G csf, by Eve Technologies (1 mentions) Gm csf, by Eve Technologies (1 mentions) Mcp 1, by Eve Technologies (1 mentions) Mip 1β, by Eve Technologies (1 mentions) Vegf a, by Eve Technologies (1 mentions) Il 12 p70 high sensitivity elisa kit, by Thermo Fisher Scientific (1 mentions)

9 protocols using human duoset elisa development kit

1

Cytokine Profiling of PBMC Cultures

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The production of IL-17, IFN-γ, IL-10, IL-8, TNF-α, IL-6, IL-1β and MCP-1 from PBMC culture supernatants was detected by using the human Duoset ELISA development kit (R&D Systems, Minneapolis, Minnesota, USA) based on the manufacturer’s protocols.
Xu D., Hou S., Zhang J., Jiang Y., Kijlstra A, & Yang P. (2015). Copy number variations and gene polymorphisms of Complement components in ocular Behcet’s disease and Vogt-Koyanagi-Harada syndrome. Scientific Reports, 5, 12989.
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2

Cytokine Profiling of Stimulated PBMCs

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The supernatants of stimulated PBMCs were collected to test the concentration of cytokines (IFN-γ, IL-10, and IL-17) by the human Duoset ELISA development kit (R&D Systems, Minneapolis, USA).
Jiang Y., Wang H., Yu H., Li L., Xu D., Hou S., Kijlstra A, & Yang P. (2016). Two Genetic Variations in the IRF8 region are associated with Behçet’s disease in Han Chinese. Scientific Reports, 6, 19651.
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3

Cytokine Secretion Quantification in PBMCs

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The concentration of IL-1β, TNF-α, IL-6, IL-10, IL-17 and IFN-γ in the culture supernatants of LPS or anti-CD3 combined with anti-CD28 antibodies treated PBMCs was measured using the human Duoset ELISA development kit (R&D Systems, Minneapolis, Minnesota, USA). Recombinant proteins were used to generate the standard curve. Test results were evaluated using an ELISA reader (SpectraMax M2e, Molecular Devices, USA) at 450 nm.
Qin J., Li L., Zhang D., Yu H., Tan H., Zhang J., Deng B., Kijlstra A, & Yang P. (2016). Analysis of receptor tyrosine kinase genetics identifies two novel risk loci in GAS6 and PROS1 in Behçet’s disease. Scientific Reports, 6, 26662.
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4

Quantification of Cytokine Levels in PBMC Supernatants

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The concentration of IFN-γ, IL-6, IL-8, IL-1β, IL-10 and TNF-α in the supernatants of PBMCs were analyzed through using the human Duoset ELISA development kit (R&D Systems, Minneapolis, Minnesota, USA).
Yang Y., Tan H., Deng B., Yu H., Su G., Hu J., Cao Q., Yuan G., Kijlstra A, & Yang P. (2017). Genetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population. Scientific Reports, 7, 5348.
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5

Cytokine Quantification in PBMC Cultures

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The concentration of IL-1beta, IL-6, IL-8, IL-10, TNF-α and MCP-1 in PBMC culture supernatants was detected by the human Duoset ELISA development kit (R&D Systems, Minneapolis, Minnesota, USA) based upon the manufacturer’s protocols.
Zheng M., Zhang L., Yu H., Hu J., Cao Q., Huang G., Huang Y., Yuan G., Kijlstra A, & Yang P. (2016). Genetic polymorphisms of cell adhesion molecules in Behcet’s disease in a Chinese Han population. Scientific Reports, 6, 24974.
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6

ELISA Quantification of Cytokines

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Supernatants of stimulated PBMCs and transfected RPE cells were stored at −80 °C until cytokine measurement. The concentration of IL-6, IL-8 and MCP-1 in the supernatants was measured with a human Duoset ELISA development kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s protocols.
Hou S., Ye Z., Liao D., Bai L., Liu Y., Zhang J., Kijlstra A, & Yang P. (2016). miR-23a, miR-146a and miR-301a confer predisposition to Vogt-Koyanagi-Harada syndrome but not to Behcet’s disease. Scientific Reports, 6, 20057.
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7

ELISA Measurement of Inflammatory Cytokines

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The concentrations of TNF-α, IL-6, and IL-1β in cell supernatants were measured by ELISA (Human DuoSet ELISA Development Kit; R&D Systems, Minneapolis, MN).
Liang L., Zhou Q, & Feng L. (2021). Decreased microRNA-155 in Behcet’s disease leads to defective control of autophagy thereby stimulating excessive proinflammatory cytokine production. Arthritis Research & Therapy, 23, 135.
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8

Multiplex Cytokine Profiling of Cell Supernatants

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Cell supernatants were collected and frozen down at -80 °C until assayed using a Human 42 Multiplex Discovery Assay with Luminex xMAP technology (EGF, Eotaxin-1, FGF-2, Flt-3L, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-18, IP-10, MCP-1, MCP-3, MDC, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, sCD40L, TGFα, TNFα, TNFβ, VEGF-A; Eve Technologies, AB, Canada). The level of VEGF, IL-6, and PGE2 in cell culture supernatants were also evaluated using ELISA (Human DuoSet ELISA Development Kits (VEGF, IL-6, PGE2), R&D Systems) with absorbance measured at 450 nm/570 nm.
Allen A., Vaninov N., Li M., Nguyen S., Igo P., Tilles A.W., O’Rourke B., Miller B.L., Parekkadan B, & Barcia R.N. (2020). Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells. Scientific Reports, 10, 10142.
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9

Cytokine Profiling by ELISA

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The protein levels of IL-1b, IL-6, TNF-a, IL-17, and IFN-c in culture supernatants were measured by human Duoset ELISA development kits (R&D Systems, Minneapolis, MN, USA). The IL-12p70 high sensitivity ELISA kit (eBioscience) was used to detect the concentration of IL-12p70.
Yi S., Chang R., Hu J., Qiu Y., Wang Q., Cao Q., Yuan G., Su G., Zhou C., Wang Y., Kijlstra A, & Yang P. (2018). Disabled-2 (DAB2) Overexpression Inhibits Monocyte-Derived Dendritic Cells' Function in Vogt-Koyanagi-Harada Disease. Investigative ophthalmology & visual science, 59(11).
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