Urine concentrations of human lipocalin-2/NGAL, TIM-1/KIM-1/HAVCR and Cystatin C were measured using the immunoenzymatic ELISA method (Quantikine High Sensitivity Human by R&D Systems, Minneapolis, MN, USA) according to manufacturer’s protocol. Minimum detectable concentrations were determined by the manufacturer as 0.012 ng/mL, 0.009 ng/mL, and 0.102 ng/mL, respectively. Intraassay (3.6% for lipocalin-2/NGAL; 4.3% for TIM-1/KIM-1/HAVCR; 6.6% for Cystatin C) and interassay (7.9% for lipocalin-2/NGAL; 6.3% for TIM-1/KIM-1/HAVCR; 7.0% for Cystatin C) respectively. Precisions performances of the assays were determined on 20 replicates from the quality control data of the laboratory. The absorbance were read on the automated plate reader ChroMate 4300 (Awareness Technology, Inc., Palm City, FL, USA) at the wavelength λ = 450 nm. The reference curve was prepared according to the manufacturer’s recommendations.
Quantikine high sensitivity human
Manufactured by R&D Systems
Sourced in United States
The Quantikine High Sensitivity Human assay is a quantitative sandwich enzyme immunoassay designed to measure low concentrations of target analytes in cell culture supernates, serum, and plasma. The assay provides a precise and sensitive method for the quantification of the specified analyte.
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Lab products found in correlation
4 protocols using quantikine high sensitivity human
1
Urine Biomarkers for Kidney Injury
2
Urine Biomarkers for Kidney Injury Assessment
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Urine concentrations of human lipocalin-2/NGAL, TIM-1/KIM-1/HAVCR, and Cystatin C were measured using the immunoenzymatic ELISA method (Quantikine High Sensitivity Human by R&D Systems, Minneapolis, Minn, United States) according to the manufacturer’s protocol. Minimum and maximum detectable concentrations were determined by the manufacturer as 0.012–40 ng/ml, 0.009–10 ng/ml, and 0.102–200 ng/ml, respectively.
Intraassay (3.6% for lipocalin-2/NGAL; 4.3% for TIM-1/KIM-1/HAVCR; 6.6% for Cystatin C) and interassay (7.9% for lipocalin-2/NGAL; 6.3% for TIM-1/KIM-1/HAVCR; 7.0% for Cystatin C) respectively. The absorbances were read on the automated plate reader ChroMate 4,300 (Awareness Technology, Inc, United States) at the wavelength λ = 450 nm. The reference curve was prepared according to the manufacturer’s recommendations.
Intraassay (3.6% for lipocalin-2/NGAL; 4.3% for TIM-1/KIM-1/HAVCR; 6.6% for Cystatin C) and interassay (7.9% for lipocalin-2/NGAL; 6.3% for TIM-1/KIM-1/HAVCR; 7.0% for Cystatin C) respectively. The absorbances were read on the automated plate reader ChroMate 4,300 (Awareness Technology, Inc, United States) at the wavelength λ = 450 nm. The reference curve was prepared according to the manufacturer’s recommendations.
3
Quantification of Serum IL12 by ELISA
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Blood samples were immediately placed on ice, clarified by centrifugation at 3.000g for 5 min at 4°C, and kept frozen at −80°C until assayed. Serum level of IL12 was measured by immunoenzymatic ELISA method (Quantikine High Sensitivity Human by R&D Systems, Minneapolis, MN, USA) according to manufacturer protocol. Minimum detectable concentrations were determined by the manufacturer as 1.0 pg/ml. Intra-assay was 1.1% and interassay 7.1%. Precisions performances of the assays were determined on 20 replicates from the quality control data of the laboratory. Absorbance was read at 450 nm on an automated plate reader ChroMate 4300 (Awareness Technology, Inc., USA). The reference curve was prepared according to the manufacturer's recommendations.
4
Quantification of HTRA Proteins and IL-12
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The HTRA proteins and anti-HTRA IgGs were detected using enzyme linked immunosorbent assay (ELISA) and confirmed by western blotting. ELISA with high-binding plates coated with appropriate recombinant HTRA protein (a capture antigen) and rabbit polyclonal antibodies to the human HTRA1-3 proteins were used. To visualize binding of the human or rabbit anti-HTRA antibodies to a recombinant HTRA protein, SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel) electrophoresis of the protein was performed according to Laemmli et al. [28 (link)]. Subsequently, western blotting was performed as described by Harlow et al. [29 ], using the anti-human IgG or anti-rabbit IgG antibodies.
Serum Il-12 levels were measured by immunoenzymatic ELISA method (Quantikine High Sensitivity Human by R&D Systems, Minneapolis, MN, USA) according to manufacturer protocol. Minimum detectable concentrations were determined by the manufacturer as 1.0 pg/ml. Intra-assay and inter-assay coefficient of variation (CV) was 1.2% and 7.1%, respectively. Precision performances of the assays were determined on 20 replicates from the quality control data of the laboratory. Absorbance was read at 450 nm on an automated plate reader (ChroMate 4300, Awareness Technology, Inc., Palm City, FL, USA). The reference curve was prepared according to the manufacturer’s recommendations.
Serum Il-12 levels were measured by immunoenzymatic ELISA method (Quantikine High Sensitivity Human by R&D Systems, Minneapolis, MN, USA) according to manufacturer protocol. Minimum detectable concentrations were determined by the manufacturer as 1.0 pg/ml. Intra-assay and inter-assay coefficient of variation (CV) was 1.2% and 7.1%, respectively. Precision performances of the assays were determined on 20 replicates from the quality control data of the laboratory. Absorbance was read at 450 nm on an automated plate reader (ChroMate 4300, Awareness Technology, Inc., Palm City, FL, USA). The reference curve was prepared according to the manufacturer’s recommendations.
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