Mirna specific primers

RNAiso (Takara) extracted total RNA from the kidney tissue and HK-2 cells. After that, we collected 10 ng RNA for further analysis. Mature miRNAs were quantified using miRNA-specific primers (GeneCopoeia) real-time PCR assay kit. U6 was selected as internal standards for gene expression, respectively. Relative gene expression was calculated using the 2^-ΔΔCT formula.

For mRNA, total RNA was extracted and 500 ng of RNA was used for cDNA synthesis using the Takara reverse transcription kit (Takara, Dalian, China) according to the manufacturer’s instructions. PCR was conducted using SYBR Premix Ex Taq (Takara, Dalian, China) according to the manufacturer’s instructions on an Mx3000P system (Agilent Stratagene, Santa Clara, CA, USA). The primers were chemically synthesized by Tsingke, Wuhan, China and are listed in Table S1. The All-in-One™ miRNA qRT-PCR Detection Kit (GeneCopoeia, Guangzhou, China) was used for both cDNA synthesis and quantitative detection using miRNA specific primers (GeneCopoeia, Guangzhou, China). GAPDH and U6 were used as internal controls to determine the relative expression of target mRNA and miRNA. All reactions were performed in triplicate.

The total RNA from the kidney tissue, HK-2, hucMSCs, or exosomes was extracted with RNAiso (Takara). mRNA was quantified using PrimeScript reverse transcription reagent and real-time PCR (RT-PCR) kit with SYBR Green (Takara). Mature miRNAs were quantified with the real-time PCR detection kit using miRNA-specific primers (GeneCopoeia). All the primer sequences were listed in Table S1.