To reveal the proportional difference in the two subsets of γδ T cells from peripheral blood mononuclear cells (PBMCs) between the healthy population and HCC patients, the raw immune-phenotyping data of these two populations provided by the Shuangzhi Purui Medical Laboratory Co., Ltd. (Wuhan, China) were obtained and analyzed. The healthy population consisted of 170 disease-free adults (age range, 26–72; gender includes both female and male). The HCC population consisted of 42 patients (age range, 30–71; diagnosis, HCC I to IV including both primary and recurrence; gender includes both female and male). The antibodies used included anti-human CD3, anti-human TCR Vδ1, and anti-human TCR Vδ2 antibodies (BioLegend, San Diego, CA, USA). All examinations were conducted using BD FACSCanto cytometry (BD Biosciences, San Jose, CA, USA).
Anti human tcr vδ2
Manufactured by BioLegend
Sourced in United States
The Anti-human TCR Vδ2 is a laboratory reagent used to detect and analyze the presence of the Vδ2 chain of the T cell receptor (TCR) on human T cells. It is a specific antibody that binds to the Vδ2 region of the TCR.
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Lab products found in correlation
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Anti human cd45, by BioLegend (1 mentions)
Anti human ccr2, by BioLegend (1 mentions)
Anti human granzyme b, by BioLegend (1 mentions)
Anti human perforin, by BioLegend (1 mentions)
Lsrii cytometer, by BD (1 mentions)
2 protocols using anti human tcr vδ2
1
Profiling γδ T Cell Subsets in HCC
2
Profiling Urine Cell Immune Signatures
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Cryopreserved patient post-BCG urine cells were thawed and resuspended in cR-10 media at 10×106 cells/mL then passed through 100 µm strainer. Urine cell suspensions were either directly stained with flow cytometry antibody (for T cells and activation markers) or incubated with PMA/Ionomycin/Golgi Blocker (BioLegend, Cell Activation Cocktail) for 4 hours, followed by cell surface staining for T cells and then intracellular staining for cytokines. Flow staining antibodies/dye used were as follows: Fc blocker/Human TruStain FcX (BioLegend), fixable viability dye eFluor455UV (Thermo Fisher Scientific/eBioscience), anti-human CD45 (BioLegend, clone HI30), anti-human CD3, anti-human CD4, anti-human CD8, anti-human γδ TCR, anti-human TCR Vγ9 (BioLegend, clone B3), anti-human TCR Vδ2 (BioLegend, clone B6), anti-human CCR2 (BioLegend, clone KO36C2), anti-human CD44 (BioLegend, clone IM7), anti-human CD107a, anti-human CD56 (BioLegend, clone 5.1H11), anti-human IFNγ, anti-human TNFα, anti-human Granzyme B (BioLegend, clone GB11), anti-human Perforin (BioLegend, clone dG9). Fixed and stained urine cell samples were then passed through a 35 µm filter cap on a flow tube to remove any remaining debris before they were analyzed on an LSR II cytometer (BD).
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