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5 protocols using fecl2 6h2o

1

Synthesis and Characterization of Folate-Decorated Nanoparticles

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GO, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N, N′-Dicyclohexylcarbodiimide (DCC), Chloroauric acid (HAuCl4), and Folic acid (FA), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and fetal bovine serum (FBS) were purchased from Sigma Aldrich, USA. Polyethylene glycol (PEG6000) was purchased from ROTH, Germany. 4- Dimethylaminopyridine (DMAP), Dimethyl sulfoxide (DMSO), sodium citrate, FeCl3·6H2O and FeCl2·6H2O received from Merck, Germany. Dulbecco’s Modified Eagle Medium (DMEM), Penicillin/Streptomycin (Pen/Strep), Trypsin–EDTA enzyme were provided by Gibco, USA.
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2

Sensitive Determination of Furfurals in Baby Food

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LC-grade acetonitrile and methanol, 2-propanol, ethyl acetate, ethanol, acetone, FeCl2·6H2O, 1,4-benzene dicarboxylic acid (H2BDC), FeCl3, potassium hexacyanoferrate, p-phenylenediamine (PDA), ammonium persulfate (APS), ammonium hydroxide (28% w/v), sodium hydroxide, NaCl, zinc acetate dihydrate (Zn(OAc)2 2H2O), acetic acid (HOAc), and N,N-dimethylformamide (DMF) were obtained from Merck (Darmstadt, Germany). Deionized water (DI) was prepared by utilizing a Milli-Q system from Millipore (MA, USA). Baby food and dry milk powder samples were purchased from Tehran supermarkets/drug stores (Tehran, Iran) and refrigerated at 4 °C before analysis.
F and HMF (analytical-grade) were obtained from Sigma-Aldrich (Germany). Stock solutions of F and HMF (1000 μg mL−1) were prepared in methanol. Working mixed solutions of furfurals were prepared daily by diluting appropriate volumes of the stock solutions in DI water. Carrez solutions I and II were used for sample preparation. Carrez solution I was fabricated by dissolving 10.6 g K3[Fe(CN)6] in 100 mL DI water. To prepare Carrez solution II, in brief, 21.9 g Zn(OAc)2 along with 3 mL HOAc were mixed in a 100 mL volumetric flask, and then its volume was adjusted with DI water.
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3

Quantitative Analysis of F and HMF in Milk/Baby Food

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F and HMF (analytical-grade), acetonitrile and methanol (HPLC-grade), ethyl acetate, ethanol (EtOH), isopropanol, acetone, FeCl3·6H2O, FeCl2·6H2O, terephthalic acid (H2BDC), K3[Fe(CN)6], aniline, ammonium persulfate, pyrrole, NaOH, NaCl, (Zn(OAc)2), N,N-dimethylformamide (DMF), acetic acid, and NH4OH solution (25% w/v) were purchased from Merck (Darmstadt, Germany) or Sigma-Aldrich (Germany). A Milli-Q system (Millipore, MA, USA) was employed to prepare deionized water (DI). The real samples (dried milk/baby food) were obtained from the local drug stores (Tehran, Iran) and maintained in a refrigerator (4 °C) before analysis.
Stock solutions of F and HMF (1000 mg L−1) were prepared in HPLC-grade methanol. The mixed working solutions were prepared by dilution of the proper volumes of the stock solution in DI water. To prepare the Carrez solution, 10.6 g K3[Fe(CN)6] was dissolved in 100 mL DI water. Carrez solution II was obtained by mixing 21.9 g Zn(OAc)2 and 3 mL HOAc in a 100 mL volumetric flask and adjusting the volume by DI water.
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4

Synthesis of Magnetic Silica Nanoparticles

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All chemicals including NiCl2·6H2O (Merck, Darmstadt, Germany, puriss.p.a. >98%), FeCl3·6H2O (Merck, reagent grade > 98%), FeCl2·6H2O (Merck, puriss.p.a. > 99%), tetraethylorthosilicate Si(OEt)4 (TEOS) (Merck, reagent grade 98%), trimethoxy(3-(oxiran-2-ylmethoxy)propyl)silane (Sigma-Aldrich, St. Louis, MO, USA, MW = 248.35 g/mol, 99%), PEI (Sigma-Aldrich, 5000 average molecular weight, 99%), and toluene (Merck, anhydrous 99.8%) were used without further purification.
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5

Synthesis and Characterization of Iron-based Dyes

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Analytical-grade FeCl2.6H2O and FeCl3.6H2O were used as received from Merck. Methylene blue (Mw = 319.85 g mol−1, molecular formula C16H18ClN3S, and λmax = 664 nm) was supplied by Alvan Co., Iran (Tehran, Iran). A Smart-2-Pure, TKACo ultrapure water instrument was used for preparing deionized water. Crystal violet dye (dye content ≥ 90%, CI = 42,555, MW = 407.99 g mol−1 as well as λmax = 590 nm, molecular formula C25H30ClN3) was procured from Merck Co. (Darmstadt, Germany).
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